Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)

Detalhes bibliográficos
Autor(a) principal: Brandão,Aline Dias
Data de Publicação: 2009
Outros Autores: Viccini,Lyderson Facio, Recco-Pimentel,Shirlei Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400013
Resumo: Since previous cytogenetic reports of Aloysia have only described the meiotic behavior and chromosomal number of some species, the aim of this work was to provide detailed cytogenetic description of Aloysia virgata that would contribute to the understanding of the taxonomical organization of the Verbenaceae. Aloysia virgata had a karyotype with 2n = 36 metacentric chromosomes, all with similar size. The large amount of heterochromatin seen after Giemsa staining was confirmed by C-banding. Four nucleolar organizer regions (NORs) were detected with an rDNA 45S probe in two homologous pairs and two sites of 5S rDNA located on one chromosomal pair were detected by fluorescence in situ hybridization. The interphase nucleus was classified as semi-reticulate. Meiotic analysis showed a normal chromosomal behavior, with 18 bivalents in some parts of prophase I and in metaphase I. The number of chromosomes, NORs and 5S rDNA segments did not exclude a possible polyploid origin.
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spelling Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)ChromosomeCytogenetic characterizationFISHAloysiaVerbenaceaeSince previous cytogenetic reports of Aloysia have only described the meiotic behavior and chromosomal number of some species, the aim of this work was to provide detailed cytogenetic description of Aloysia virgata that would contribute to the understanding of the taxonomical organization of the Verbenaceae. Aloysia virgata had a karyotype with 2n = 36 metacentric chromosomes, all with similar size. The large amount of heterochromatin seen after Giemsa staining was confirmed by C-banding. Four nucleolar organizer regions (NORs) were detected with an rDNA 45S probe in two homologous pairs and two sites of 5S rDNA located on one chromosomal pair were detected by fluorescence in situ hybridization. The interphase nucleus was classified as semi-reticulate. Meiotic analysis showed a normal chromosomal behavior, with 18 bivalents in some parts of prophase I and in metaphase I. The number of chromosomes, NORs and 5S rDNA segments did not exclude a possible polyploid origin.Instituto de Tecnologia do Paraná - Tecpar2009-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400013Brazilian Archives of Biology and Technology v.52 n.4 2009reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132009000400013info:eu-repo/semantics/openAccessBrandão,Aline DiasViccini,Lyderson FacioRecco-Pimentel,Shirlei Mariaeng2009-09-10T00:00:00Zoai:scielo:S1516-89132009000400013Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-09-10T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
title Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
spellingShingle Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
Brandão,Aline Dias
Chromosome
Cytogenetic characterization
FISH
Aloysia
Verbenaceae
title_short Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
title_full Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
title_fullStr Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
title_full_unstemmed Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
title_sort Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae)
author Brandão,Aline Dias
author_facet Brandão,Aline Dias
Viccini,Lyderson Facio
Recco-Pimentel,Shirlei Maria
author_role author
author2 Viccini,Lyderson Facio
Recco-Pimentel,Shirlei Maria
author2_role author
author
dc.contributor.author.fl_str_mv Brandão,Aline Dias
Viccini,Lyderson Facio
Recco-Pimentel,Shirlei Maria
dc.subject.por.fl_str_mv Chromosome
Cytogenetic characterization
FISH
Aloysia
Verbenaceae
topic Chromosome
Cytogenetic characterization
FISH
Aloysia
Verbenaceae
description Since previous cytogenetic reports of Aloysia have only described the meiotic behavior and chromosomal number of some species, the aim of this work was to provide detailed cytogenetic description of Aloysia virgata that would contribute to the understanding of the taxonomical organization of the Verbenaceae. Aloysia virgata had a karyotype with 2n = 36 metacentric chromosomes, all with similar size. The large amount of heterochromatin seen after Giemsa staining was confirmed by C-banding. Four nucleolar organizer regions (NORs) were detected with an rDNA 45S probe in two homologous pairs and two sites of 5S rDNA located on one chromosomal pair were detected by fluorescence in situ hybridization. The interphase nucleus was classified as semi-reticulate. Meiotic analysis showed a normal chromosomal behavior, with 18 bivalents in some parts of prophase I and in metaphase I. The number of chromosomes, NORs and 5S rDNA segments did not exclude a possible polyploid origin.
publishDate 2009
dc.date.none.fl_str_mv 2009-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132009000400013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.52 n.4 2009
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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