Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation

Detalhes bibliográficos
Autor(a) principal: Michelena,Georgina L.
Data de Publicação: 2003
Outros Autores: Martínez,Aidín, Bell,Antonio, Carrera,Emilia, Valencia,Roxana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300017
Resumo: Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate.
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spelling Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentationLeuconostoc mesenteroidesdextransucrase enzymescale-upoxygen transferfed batch fermentationFed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate.Instituto de Tecnologia do Paraná - Tecpar2003-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300017Brazilian Archives of Biology and Technology v.46 n.3 2003reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132003000300017info:eu-repo/semantics/openAccessMichelena,Georgina L.Martínez,AidínBell,AntonioCarrera,EmiliaValencia,Roxanaeng2003-11-18T00:00:00Zoai:scielo:S1516-89132003000300017Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2003-11-18T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
title Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
spellingShingle Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
Michelena,Georgina L.
Leuconostoc mesenteroides
dextransucrase enzyme
scale-up
oxygen transfer
fed batch fermentation
title_short Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
title_full Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
title_fullStr Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
title_full_unstemmed Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
title_sort Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation
author Michelena,Georgina L.
author_facet Michelena,Georgina L.
Martínez,Aidín
Bell,Antonio
Carrera,Emilia
Valencia,Roxana
author_role author
author2 Martínez,Aidín
Bell,Antonio
Carrera,Emilia
Valencia,Roxana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Michelena,Georgina L.
Martínez,Aidín
Bell,Antonio
Carrera,Emilia
Valencia,Roxana
dc.subject.por.fl_str_mv Leuconostoc mesenteroides
dextransucrase enzyme
scale-up
oxygen transfer
fed batch fermentation
topic Leuconostoc mesenteroides
dextransucrase enzyme
scale-up
oxygen transfer
fed batch fermentation
description Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate.
publishDate 2003
dc.date.none.fl_str_mv 2003-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132003000300017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.46 n.3 2003
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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