Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNICENTRO |
Texto Completo: | http://tede.unicentro.br:8080/jspui/handle/jspui/667 |
Resumo: | Introduction: In recent years diabetes has been gaining major, standing out as a worldwide epidemic. It is a disorder characterized by hyperglycemia and is associated with several complications, dysfunction and failure of various organs. May result from defects in secretion and/or insulin action involving specific pathogenic processes. Due to the large number of cases , the disease causes a huge burden on the public purse. In this sense , special attention has been given to the different levels of disease prevention. To this end, several plant species have been used to treat and or combat the symptoms of diabetes mellitus . The present study utilized methanol extract of Baccharis dracunculifolia popularly known as broom or rosemary - of - field , is widely used in folk medicine . The infusion of the leaves is used for liver problems, stomach disorders and how antiinflammatory. Methods: 24 adult Wistar rats treated for 28 days were used. The animals were divided into 4 groups as follows: CS - Saline Control (treated with a solution of "Tween 80" and distilled water) CP - Control plant (treated with the extract of B. dracunculifolia); DS - Diabetic Saline (induced diabetes , treated with a solution of "Tween 80" and distilled water), SD - diabetic Plant (induced diabetes treated with extract of B. dracunculifolia) . The induction of diabetes was performed by administering 50 mg / kg streptozotocin (STZ). The animals were evaluated for weight gain. At 14◦ day of treatment the animals underwent OGTT, drop of blood flow. At the end of the treatment the animals were euthanized for withdrawal of blood samples and tissues for determination of biochemical parameters, hormonal and preparation of histological slides and quantification of TBARS via malondialdehyde in renal and hepatic tissue. Results and Discussion: There was statistical difference for the DS and DP groups parameters for creatinine (p <0,05) , urea (p < 0.01), and triglycerides (p < 0:01). For creatinine and triglycerides analytes DP was considered statistically equal to the nondiabetic groups (CS and CP). Plasma insulin was statistically significant (p < 0.01), between the diabetic groups, with higher concentrations for the DP . DP on the OGTT , fasting , was statistically comparable with nondiabetic controls (CP and CS) , the following measurements SD was statistically different from all groups , with intermediate values between diabetic nondiabetic groups (CP and CS) and the control group (SD) . The PD group showed a significant reduction in lipid peroxidation when compared to the DS group, this reduction comparable to the non-diabetic groups . The diabetic group treated with extract showed less severe tissue damage when compared to the DS group. Groups of non-diabetic animals (CS and CP) had normal histology. Conclusion: The methanol extract of Baccharis dracunculifolia promoted the decrease of capillary glucose levels of fasting rats induced by STZ diabetes , a decreased value of triglycerides , creatinine and urea STZ -induced animals , the plant extract showed protection from damage to organs (liver , kidneys and pancreas ) induced diabetic animals ; occurred statistical difference in insulin release in diabetic animals where treated animals had higher concentrations. |
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MALFATTI, CARLOS RICARDO MANECKhttp://lattes.cnpq.br/7879558601666787SILVA, WEBER CLAÚDIO FRANCISCO NUNES DAhttp://lattes.cnpq.br/1892865713778963033.279.769-45http://lattes.cnpq.br/2122103878458333PEREIRA, RICARDO APARECIDO2017-06-05T19:11:47Z2014-03-14PEREIRA, RICARDO APARECIDO. Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes. 2014. 56 f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - Mestrado / Associação Ampla com UEPG) - Universidade Estadual do Centro-Oeste, Guarapuava - PR.http://tede.unicentro.br:8080/jspui/handle/jspui/667Introduction: In recent years diabetes has been gaining major, standing out as a worldwide epidemic. It is a disorder characterized by hyperglycemia and is associated with several complications, dysfunction and failure of various organs. May result from defects in secretion and/or insulin action involving specific pathogenic processes. Due to the large number of cases , the disease causes a huge burden on the public purse. In this sense , special attention has been given to the different levels of disease prevention. To this end, several plant species have been used to treat and or combat the symptoms of diabetes mellitus . The present study utilized methanol extract of Baccharis dracunculifolia popularly known as broom or rosemary - of - field , is widely used in folk medicine . The infusion of the leaves is used for liver problems, stomach disorders and how antiinflammatory. Methods: 24 adult Wistar rats treated for 28 days were used. The animals were divided into 4 groups as follows: CS - Saline Control (treated with a solution of "Tween 80" and distilled water) CP - Control plant (treated with the extract of B. dracunculifolia); DS - Diabetic Saline (induced diabetes , treated with a solution of "Tween 80" and distilled water), SD - diabetic Plant (induced diabetes treated with extract of B. dracunculifolia) . The induction of diabetes was performed by administering 50 mg / kg streptozotocin (STZ). The animals were evaluated for weight gain. At 14◦ day of treatment the animals underwent OGTT, drop of blood flow. At the end of the treatment the animals were euthanized for withdrawal of blood samples and tissues for determination of biochemical parameters, hormonal and preparation of histological slides and quantification of TBARS via malondialdehyde in renal and hepatic tissue. Results and Discussion: There was statistical difference for the DS and DP groups parameters for creatinine (p <0,05) , urea (p < 0.01), and triglycerides (p < 0:01). For creatinine and triglycerides analytes DP was considered statistically equal to the nondiabetic groups (CS and CP). Plasma insulin was statistically significant (p < 0.01), between the diabetic groups, with higher concentrations for the DP . DP on the OGTT , fasting , was statistically comparable with nondiabetic controls (CP and CS) , the following measurements SD was statistically different from all groups , with intermediate values between diabetic nondiabetic groups (CP and CS) and the control group (SD) . The PD group showed a significant reduction in lipid peroxidation when compared to the DS group, this reduction comparable to the non-diabetic groups . The diabetic group treated with extract showed less severe tissue damage when compared to the DS group. Groups of non-diabetic animals (CS and CP) had normal histology. Conclusion: The methanol extract of Baccharis dracunculifolia promoted the decrease of capillary glucose levels of fasting rats induced by STZ diabetes , a decreased value of triglycerides , creatinine and urea STZ -induced animals , the plant extract showed protection from damage to organs (liver , kidneys and pancreas ) induced diabetic animals ; occurred statistical difference in insulin release in diabetic animals where treated animals had higher concentrations.Introdução: Nos últimos anos o Diabetes vem ganhando grandes proporções, destacando-se como uma epidemia mundial. É uma patologia caracterizada por hiperglicemia e esta associada a diversas complicações, disfunções e insuficiência de vários órgãos. Pode resultar de defeitos de secreção e/ou ação da insulina envolvendo processos patogênicos específicos. Devido ao grande número de casos, a doença causa um enorme ônus aos cofres públicos. Nesse sentido, atenção especial tem sido dispensada aos diferentes níveis preventivos da doença. Para tanto, várias espécies de plantas vêm sendo utilizadas para tratar e ou combater os sintomas do Diabetes mellitus. O presente trabalho utilizou extrato metanólico de Baccharis dracunculifolia popularmente conhecida como vassoura ou alecrim-do-campo, é amplamente utilizada na medicina caseira. A infusão de suas folhas é empregada para problemas hepáticos, disfunções estomacais e como antiinflamatório.Métodos: Foram utilizados 24 ratos adultos da linhagem Wistar tratados por 28 dias. Os animais foram divididos em 4 grupos da seguinte maneira: CS – Controle Salina (tratados com solução de “Tween 80” e água destilada); CP – Controle Planta (tratados com o extrato de B. dracunculifolia); DS – Diabético Salina (induzidos ao diabetes, tratados com solução de “Tween 80” e água destilada) e; DP – Diabético Planta (induzidos ao diabetes, tratados com o extrato de B. dracunculifolia) . A indução do diabetes foi realizada pela administração de 50 mg/kg de estreptozotocina (STZ). Os animais foram avaliados quanto a evolução ponderal. No 14◦ dia de tratamento os animais passaram por TOTG, gota de sangue caudal. Ao final do tratamento os animais foram eutanasiados para a retirada de amostras de sangue e tecidos para determinação de parâmetros bioquímicos, hormonais e confecção de lâminas histológicas e quantificação de TBARS via malondialdeído em tecido renal e hepático. Resultados e Discussão: Verificou-se diferença estatística para os grupos DS e DP para parâmetros creatinina (p<0.05), uréia (p<0.01) e triglicérides (p<0.01). Para os analitos creatinina e triglicérides DP foi considerado estatisticamente igual aos grupos não diabéticos (CS e CP). A insulina plasmática apresentou significância estatística (p <0.01) entre os grupos diabéticos, com concentrações mais elevadas para o DP. No TOTG DP, ao jejum, foi comparável estatisticamente com os controles não diabéticos (CP e CS), nas quantificações seguintes DP foi estatisticamente diferente de todos os grupos, apresentando valores intermediários entre os grupos não diabéticos (CP e CS) e o grupo controle diabético (DS). O grupo DP apresentou uma redução significativa na peroxidação lipídica quando comparado ao grupo DS, redução essa comparável com os grupos não diabéticos. O grupo diabético tratado com extrato apresentou dano tecidual de menor intensidade quando comparado ao grupo DS. Os grupos de animais não diabéticos (CS e CP) não apresentaram alterações histológicas Conclusão: O extrato metanólico de Baccharis dracunculifolia promoveu queda das níveis de glicose capilar ao jejum de ratos induzidos a diabetes por STZ; Ocorreu queda de valores de triglicerídeos, creatinina e uréia de animais STZ induzidos; O extrato da planta apresentou proteção de dano em órgãos (fígado, rins e pâncreas) de animais diabéticos induzidos; Ocorreu diferença estatística na liberação de insulina entre os animais diabéticos, onde os animais tratados obtiveram concentrações superiores.Submitted by Fabiano Jucá (fjuca@unicentro.br) on 2017-06-05T19:11:47Z No. of bitstreams: 1 RICARDO APARECIDO PEREIRA.pdf: 1595029 bytes, checksum: 2eb8332aa963158acb2d1f384fd9d026 (MD5)Made available in DSpace on 2017-06-05T19:11:47Z (GMT). No. of bitstreams: 1 RICARDO APARECIDO PEREIRA.pdf: 1595029 bytes, checksum: 2eb8332aa963158acb2d1f384fd9d026 (MD5) Previous issue date: 2014-03-14Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede.unicentro.br:8080/jspui/retrieve/2030/RICARDO%20APARECIDO%20PEREIRA.pdf.jpgporUniversidade Estadual do Centro-OestePrograma de Pós-Graduação em Ciências Farmacêuticas (Mestrado / Associação Ampla com UEPG)UNICENTROBrasilUnicentro::Departamento de FarmáciaDiabetesBaccharisGlicemiaPlantas medicinaisDiabetesBaccharisGlucoseHerbal medicineCIENCIAS DA SAUDE::FARMACIAEfeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-7679163762264962259600600600600-693476683800971729069976364134497549962075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes |
title |
Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes |
spellingShingle |
Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes PEREIRA, RICARDO APARECIDO Diabetes Baccharis Glicemia Plantas medicinais Diabetes Baccharis Glucose Herbal medicine CIENCIAS DA SAUDE::FARMACIA |
title_short |
Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes |
title_full |
Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes |
title_fullStr |
Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes |
title_full_unstemmed |
Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes |
title_sort |
Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes |
author |
PEREIRA, RICARDO APARECIDO |
author_facet |
PEREIRA, RICARDO APARECIDO |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
MALFATTI, CARLOS RICARDO MANECK |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7879558601666787 |
dc.contributor.advisor-co1.fl_str_mv |
SILVA, WEBER CLAÚDIO FRANCISCO NUNES DA |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/1892865713778963 |
dc.contributor.authorID.fl_str_mv |
033.279.769-45 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2122103878458333 |
dc.contributor.author.fl_str_mv |
PEREIRA, RICARDO APARECIDO |
contributor_str_mv |
MALFATTI, CARLOS RICARDO MANECK SILVA, WEBER CLAÚDIO FRANCISCO NUNES DA |
dc.subject.por.fl_str_mv |
Diabetes Baccharis Glicemia Plantas medicinais |
topic |
Diabetes Baccharis Glicemia Plantas medicinais Diabetes Baccharis Glucose Herbal medicine CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Diabetes Baccharis Glucose Herbal medicine |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA |
description |
Introduction: In recent years diabetes has been gaining major, standing out as a worldwide epidemic. It is a disorder characterized by hyperglycemia and is associated with several complications, dysfunction and failure of various organs. May result from defects in secretion and/or insulin action involving specific pathogenic processes. Due to the large number of cases , the disease causes a huge burden on the public purse. In this sense , special attention has been given to the different levels of disease prevention. To this end, several plant species have been used to treat and or combat the symptoms of diabetes mellitus . The present study utilized methanol extract of Baccharis dracunculifolia popularly known as broom or rosemary - of - field , is widely used in folk medicine . The infusion of the leaves is used for liver problems, stomach disorders and how antiinflammatory. Methods: 24 adult Wistar rats treated for 28 days were used. The animals were divided into 4 groups as follows: CS - Saline Control (treated with a solution of "Tween 80" and distilled water) CP - Control plant (treated with the extract of B. dracunculifolia); DS - Diabetic Saline (induced diabetes , treated with a solution of "Tween 80" and distilled water), SD - diabetic Plant (induced diabetes treated with extract of B. dracunculifolia) . The induction of diabetes was performed by administering 50 mg / kg streptozotocin (STZ). The animals were evaluated for weight gain. At 14◦ day of treatment the animals underwent OGTT, drop of blood flow. At the end of the treatment the animals were euthanized for withdrawal of blood samples and tissues for determination of biochemical parameters, hormonal and preparation of histological slides and quantification of TBARS via malondialdehyde in renal and hepatic tissue. Results and Discussion: There was statistical difference for the DS and DP groups parameters for creatinine (p <0,05) , urea (p < 0.01), and triglycerides (p < 0:01). For creatinine and triglycerides analytes DP was considered statistically equal to the nondiabetic groups (CS and CP). Plasma insulin was statistically significant (p < 0.01), between the diabetic groups, with higher concentrations for the DP . DP on the OGTT , fasting , was statistically comparable with nondiabetic controls (CP and CS) , the following measurements SD was statistically different from all groups , with intermediate values between diabetic nondiabetic groups (CP and CS) and the control group (SD) . The PD group showed a significant reduction in lipid peroxidation when compared to the DS group, this reduction comparable to the non-diabetic groups . The diabetic group treated with extract showed less severe tissue damage when compared to the DS group. Groups of non-diabetic animals (CS and CP) had normal histology. Conclusion: The methanol extract of Baccharis dracunculifolia promoted the decrease of capillary glucose levels of fasting rats induced by STZ diabetes , a decreased value of triglycerides , creatinine and urea STZ -induced animals , the plant extract showed protection from damage to organs (liver , kidneys and pancreas ) induced diabetic animals ; occurred statistical difference in insulin release in diabetic animals where treated animals had higher concentrations. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-03-14 |
dc.date.accessioned.fl_str_mv |
2017-06-05T19:11:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, RICARDO APARECIDO. Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes. 2014. 56 f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - Mestrado / Associação Ampla com UEPG) - Universidade Estadual do Centro-Oeste, Guarapuava - PR. |
dc.identifier.uri.fl_str_mv |
http://tede.unicentro.br:8080/jspui/handle/jspui/667 |
identifier_str_mv |
PEREIRA, RICARDO APARECIDO. Efeitos do tratamento do extrato metanólico de Baccharis dracunculifolia sobre alterações bioquímicas e histológicas de um modelo animal de diabetes. 2014. 56 f. Dissertação (Programa de Pós-Graduação em Ciências Farmacêuticas - Mestrado / Associação Ampla com UEPG) - Universidade Estadual do Centro-Oeste, Guarapuava - PR. |
url |
http://tede.unicentro.br:8080/jspui/handle/jspui/667 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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600 600 600 600 |
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6997636413449754996 |
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2075167498588264571 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Centro-Oeste |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas (Mestrado / Associação Ampla com UEPG) |
dc.publisher.initials.fl_str_mv |
UNICENTRO |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Unicentro::Departamento de Farmácia |
publisher.none.fl_str_mv |
Universidade Estadual do Centro-Oeste |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UNICENTRO instname:Universidade Estadual do Centro-Oeste (UNICENTRO) instacron:UNICENTRO |
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Universidade Estadual do Centro-Oeste (UNICENTRO) |
instacron_str |
UNICENTRO |
institution |
UNICENTRO |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UNICENTRO |
collection |
Biblioteca Digital de Teses e Dissertações do UNICENTRO |
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Biblioteca Digital de Teses e Dissertações do UNICENTRO - Universidade Estadual do Centro-Oeste (UNICENTRO) |
repository.mail.fl_str_mv |
repositorio@unicentro.br||fabianoqueiroz@yahoo.com.br |
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1801859368323383296 |