NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNICENTRO |
Texto Completo: | http://tede.unicentro.br:8080/jspui/handle/jspui/1836 |
Resumo: | Hesperidin is one of the main flavonoids present in citrus fruits. This compound has several beneficial properties, including antitumor properties. However, hesperidin is rapidly hydrolyzed and one of the ways to protect this compound is its nanoencapsulation. In this study, gliadin-based nanoparticles containing hesperidin were obtained by the desolvation technique and a 32 factorial design was used to optimize the formulation. Independent variables were defined as CaCl2 concentration (0.5; 1 or 2%) and stabilizing agent (Pluronic F68, Tween 80 or sodium caseinate). The dependent variables were mean diameter, polydispersion index, zeta potential and encapsulation efficiency. To determine the hesperidin content in the medium, a method for the determination of Hesperidin using UV-visible molecular absorption spectroscopy was developed and validated. The method was linear in the range of 0.27 to 100 mg/L with LD = 0.01 mg/L and LQ = 0.01 mg/L, exact with recoveries ranging from 98.8 ± 4.8 to 104 .2 ± 4.8 and accurate with 3.4% RSD. The method proved to be efficient for the determination of the free compound in suspension of nanoparticles. The optimized formulation was coated with chitosan to increase the physical stability of the nanoparticles. The final nanoparticles had a mean diameter of 321 nm and a polydispersion index of 0.217 and an oval shape. After coating, the Zeta potential was +21 mV and the encapsulation efficiency was 73%. The in vitro release profile showed that about 98% of the drug was released from the nanoparticles after 48 h. Furthermore, the nanoparticles reduced the cytotoxicity of hesperidin in healthy cells (Vero cells) and increased the cytotoxicity in tumor cells (HeLa, PC-3 and Caco-2 cells). The results showed that chitosan-coated gliadin nanoparticles are potential carriers for the delivery of hesperidin in possible treatments. |
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Quináia, Sueli Pérciohttp://lattes.cnpq.br/3256907601004018079.082.249-03http://lattes.cnpq.br/2166215507702435KELTE FILHO, IRINEO2022-02-22T16:39:32Z2021-11-26KELTE FILHO, IRINEO. NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA. 2021. 77 f. Tese (Programa de Pós-Graduação em Química - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava - PR.http://tede.unicentro.br:8080/jspui/handle/jspui/1836Hesperidin is one of the main flavonoids present in citrus fruits. This compound has several beneficial properties, including antitumor properties. However, hesperidin is rapidly hydrolyzed and one of the ways to protect this compound is its nanoencapsulation. In this study, gliadin-based nanoparticles containing hesperidin were obtained by the desolvation technique and a 32 factorial design was used to optimize the formulation. Independent variables were defined as CaCl2 concentration (0.5; 1 or 2%) and stabilizing agent (Pluronic F68, Tween 80 or sodium caseinate). The dependent variables were mean diameter, polydispersion index, zeta potential and encapsulation efficiency. To determine the hesperidin content in the medium, a method for the determination of Hesperidin using UV-visible molecular absorption spectroscopy was developed and validated. The method was linear in the range of 0.27 to 100 mg/L with LD = 0.01 mg/L and LQ = 0.01 mg/L, exact with recoveries ranging from 98.8 ± 4.8 to 104 .2 ± 4.8 and accurate with 3.4% RSD. The method proved to be efficient for the determination of the free compound in suspension of nanoparticles. The optimized formulation was coated with chitosan to increase the physical stability of the nanoparticles. The final nanoparticles had a mean diameter of 321 nm and a polydispersion index of 0.217 and an oval shape. After coating, the Zeta potential was +21 mV and the encapsulation efficiency was 73%. The in vitro release profile showed that about 98% of the drug was released from the nanoparticles after 48 h. Furthermore, the nanoparticles reduced the cytotoxicity of hesperidin in healthy cells (Vero cells) and increased the cytotoxicity in tumor cells (HeLa, PC-3 and Caco-2 cells). The results showed that chitosan-coated gliadin nanoparticles are potential carriers for the delivery of hesperidin in possible treatments.A Hesperidina é um dos principais flavonoides presentes em frutas cítricas. Esse composto apresenta diversas propriedades benéficas, dentre elas propriedades antitumorais. No entanto, a hesperidina é rapidamente hidrolisada e uma das formas de proteger esse composto é a sua nanoencapsulação. Neste estudo, nanopartículas à base de gliadina contendo hesperidina foram obtidas pela técnica de dessolvatação e um delineamento fatorial 32 foi empregado para otimizar a formulação. As variáveis independentes foram definidas como concentração de CaCl2 (0,5; 1 ou 2%) e agente estabilizador (Pluronic F68, Tween 80 ou caseínato de sódio). As variáveis dependentes foram diâmetro médio, índice de polidispersão, potencial zeta e eficiência de encapsulação. Para determinar o teor de hesperidina no meio foi desenvolvido e validado um método para a determinação de Hesperidina empregando espectroscopia de absorção molecular por UV-visível. O método mostrou-se linear na faixa de 0,27 a 100 mg/L com LD = 0,01 mg/L e LQ = 0,01 mg/L, exato com recuperações variando de 98,8 ± 4,8 até 104,2 ± 4,8 e preciso com RSD de 3,4 %. O método se mostrou eficiente para a determinação do composto livre em suspensão de nanopartículas. A formulação otimizada foi revestida com quitosana para aumentar a estabilidade física das nanopartículas. As nanopartículas finais apresentaram diâmetro médio de 321 nm e índice de polidispersão de 0,217 e forma ovalada. Após o revestimento, o potencial Zeta foi de +21 mV e a eficiência de encapsulação foi de 73%. O perfil de liberação in vitro mostrou que cerca de 98% do fármaco foi liberado das nanopartículas após 48 h. Além disso, as nanopartículas reduziram a citotoxicidade da hesperidina em células saudáveis (células Vero) e aumentaram a citotoxicidade em células tumorais (células HeLa, PC-3 e Caco-2). Os resultados mostraram que as nanopartículas de gliadina revestidas com quitosana são potenciais carreadoras para a entrega de hesperidina em possíveis tratamentos.Submitted by Fabiano Jucá (fjuca@unicentro.br) on 2022-02-22T16:39:32Z No. of bitstreams: 1 TESE - Irineo Kelte Filho.pdf: 1929007 bytes, checksum: fa63510555632fc155dcbc214358e760 (MD5)Made available in DSpace on 2022-02-22T16:39:32Z (GMT). No. of bitstreams: 1 TESE - Irineo Kelte Filho.pdf: 1929007 bytes, checksum: fa63510555632fc155dcbc214358e760 (MD5) Previous issue date: 2021-11-26Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede.unicentro.br:8080/jspui/retrieve/7954/TESE%20-%20Irineo%20Kelte%20Filho.pdf.jpgporUniversidade Estadual do Centro-OestePrograma de Pós-Graduação em Química (Doutorado)UNICENTROBrasilUnicentro::Departamento de Ciências Exatas e de TecnologiaNanopartículas proteicasHesperidinaCitotoxicidadeAntitumoraisValidação de métodoProtein nanoparticlesHesperidinCytotoxicityAntitumorsMethod validationCIENCIAS EXATAS E DA TERRA::QUIMICANANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis3800526532796635565600600600600-433806549027752903315717003253031171952075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNICENTROinstname:Universidade Estadual do Centro-Oeste (UNICENTRO)instacron:UNICENTROTHUMBNAILTESE - Irineo Kelte Filho.pdf.jpgTESE - Irineo Kelte Filho.pdf.jpgimage/jpeg2769http://localhost:8080/tede/bitstream/jspui/1836/4/TESE+-+Irineo+Kelte+Filho.pdf.jpga3bcf039ca82dd0f7d98f7d7a164f914MD54TEXTTESE - Irineo Kelte Filho.pdf.txtTESE - Irineo Kelte Filho.pdf.txttext/plain128142http://localhost:8080/tede/bitstream/jspui/1836/3/TESE+-+Irineo+Kelte+Filho.pdf.txtf401de5d71b42009cfb83ae5464574dcMD53ORIGINALTESE - Irineo Kelte Filho.pdfTESE - Irineo Kelte Filho.pdfapplication/pdf1929007http://localhost:8080/tede/bitstream/jspui/1836/2/TESE+-+Irineo+Kelte+Filho.pdffa63510555632fc155dcbc214358e760MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82003http://localhost:8080/tede/bitstream/jspui/1836/1/license.txt6544a715df32d52b08aa3def94c4dddeMD51jspui/18362022-02-24 14:12:36.999oai:localhost:jspui/1836CkxJQ0VOw4dBIERFIERJU1RSSUJVScOHw4NPIE7Dg08tRVhDTFVTSVZBCgpDb20gYSBhcHJlc2VudGHDp8OjbyBkZXN0YSBsaWNlbsOnYSwgdm9jw6ogKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIMOgIFVuaXZlcnNpZGFkZSAKRXN0YWR1YWwgZG8gQ2VudHJvLU9lc3RlIChVTklDRU5UUk8pIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyLCAgdHJhZHV6aXIgKGNvbmZvcm1lIGRlZmluaWRvIGFiYWl4byksIGUvb3UgCmRpc3RyaWJ1aXIgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIChpbmNsdWluZG8gbyByZXN1bW8pIHBvciB0b2RvIG8gbXVuZG8gbm8gZm9ybWF0byBpbXByZXNzbyBlIGVsZXRyw7RuaWNvIGUgCmVtIHF1YWxxdWVyIG1laW8sIGluY2x1aW5kbyBvcyBmb3JtYXRvcyDDoXVkaW8gb3UgdsOtZGVvLgoKVm9jw6ogY29uY29yZGEgcXVlIGEgVU5JQ0VOVFJPIHBvZGUsIHNlbSBhbHRlcmFyIG8gY29udGXDumRvLCB0cmFuc3BvciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gCnBhcmEgcXVhbHF1ZXIgbWVpbyBvdSBmb3JtYXRvIHBhcmEgZmlucyBkZSBwcmVzZXJ2YcOnw6NvLgoKVm9jw6ogdGFtYsOpbSBjb25jb3JkYSBxdWUgYSBVTklDRU5UUk8gcG9kZSBtYW50ZXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFVOSUNFTlRSTyAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBVTklDRU5UUk8sIFZPQ8OKIApERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFVOSUNFTlRSTyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unicentro.br:8080/jspui/PUBhttp://tede.unicentro.br/tde_oai/oai3.phprepositorio@unicentro.br||fabianoqueiroz@yahoo.com.bropendoar:2022-02-24T17:12:36Biblioteca Digital de Teses e Dissertações do UNICENTRO - Universidade Estadual do Centro-Oeste (UNICENTRO)false |
dc.title.por.fl_str_mv |
NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA |
title |
NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA |
spellingShingle |
NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA KELTE FILHO, IRINEO Nanopartículas proteicas Hesperidina Citotoxicidade Antitumorais Validação de método Protein nanoparticles Hesperidin Cytotoxicity Antitumors Method validation CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA |
title_full |
NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA |
title_fullStr |
NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA |
title_full_unstemmed |
NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA |
title_sort |
NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA |
author |
KELTE FILHO, IRINEO |
author_facet |
KELTE FILHO, IRINEO |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Quináia, Sueli Pércio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3256907601004018 |
dc.contributor.authorID.fl_str_mv |
079.082.249-03 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2166215507702435 |
dc.contributor.author.fl_str_mv |
KELTE FILHO, IRINEO |
contributor_str_mv |
Quináia, Sueli Pércio |
dc.subject.por.fl_str_mv |
Nanopartículas proteicas Hesperidina Citotoxicidade Antitumorais Validação de método |
topic |
Nanopartículas proteicas Hesperidina Citotoxicidade Antitumorais Validação de método Protein nanoparticles Hesperidin Cytotoxicity Antitumors Method validation CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Protein nanoparticles Hesperidin Cytotoxicity Antitumors Method validation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
Hesperidin is one of the main flavonoids present in citrus fruits. This compound has several beneficial properties, including antitumor properties. However, hesperidin is rapidly hydrolyzed and one of the ways to protect this compound is its nanoencapsulation. In this study, gliadin-based nanoparticles containing hesperidin were obtained by the desolvation technique and a 32 factorial design was used to optimize the formulation. Independent variables were defined as CaCl2 concentration (0.5; 1 or 2%) and stabilizing agent (Pluronic F68, Tween 80 or sodium caseinate). The dependent variables were mean diameter, polydispersion index, zeta potential and encapsulation efficiency. To determine the hesperidin content in the medium, a method for the determination of Hesperidin using UV-visible molecular absorption spectroscopy was developed and validated. The method was linear in the range of 0.27 to 100 mg/L with LD = 0.01 mg/L and LQ = 0.01 mg/L, exact with recoveries ranging from 98.8 ± 4.8 to 104 .2 ± 4.8 and accurate with 3.4% RSD. The method proved to be efficient for the determination of the free compound in suspension of nanoparticles. The optimized formulation was coated with chitosan to increase the physical stability of the nanoparticles. The final nanoparticles had a mean diameter of 321 nm and a polydispersion index of 0.217 and an oval shape. After coating, the Zeta potential was +21 mV and the encapsulation efficiency was 73%. The in vitro release profile showed that about 98% of the drug was released from the nanoparticles after 48 h. Furthermore, the nanoparticles reduced the cytotoxicity of hesperidin in healthy cells (Vero cells) and increased the cytotoxicity in tumor cells (HeLa, PC-3 and Caco-2 cells). The results showed that chitosan-coated gliadin nanoparticles are potential carriers for the delivery of hesperidin in possible treatments. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-11-26 |
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2022-02-22T16:39:32Z |
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KELTE FILHO, IRINEO. NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA. 2021. 77 f. Tese (Programa de Pós-Graduação em Química - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava - PR. |
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http://tede.unicentro.br:8080/jspui/handle/jspui/1836 |
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KELTE FILHO, IRINEO. NANOPARTÍCULAS DE GLIADINA CONTENDO HESPERIDINA REVESTIDAS COM QUITOSANA. 2021. 77 f. Tese (Programa de Pós-Graduação em Química - Doutorado) - Universidade Estadual do Centro-Oeste, Guarapuava - PR. |
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Universidade Estadual do Centro-Oeste |
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