Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEFS |
Texto Completo: | http://tede2.uefs.br:8080/handle/tede/927 |
Resumo: | The use of herbal medicines has become a valuable resource in its treatment of oral disorders such as fungal and bacterial infections. One of the most prevalent infectious and inflammatory diseases in the mouth is periodontitis, which if left untreated, can lead to tooth loss. This study evaluated the immunomodulatory and antimicrobial potential of Lippia alnifolia Mart & Schauer (LA) and Vatairea macrocarpa (Benth.) Ducke (VM) leaf extract. Immunological evaluation was performed by immunoassay after culturing peripheral blood cells of individuals with and without periodontitis with the plant extract and the periodontopathogen Porphyromonas gingivalis (PG) extract. Antimicrobial activity was tested with minimal inhibitory concentration and disk diffusion assay. LA extract inhibited the production of IL-13, IL-6 and IFN-γ, both in relation to the basal concentration produced by cells and the concentration produced by cells co-cultured with PG extract. Co-cultivation of MV with PG promoted a reduction in IL-1β, IL-13, IL-6, IFN-γ, IL-10 and IL-17 levels. The LA extract showed bacteriostatic activity for Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella choleraesuis, fungistatic for Candida albicans and fungicide for Candida glabrata. VM showed bactericidal activity for Micrococcus luteus species and bacteriostatic other bacteria, as well as fungistatic activity for Candida albicans and fungicide for Candida glabrata. LA and VM extracts are promising in regulating cytokine production in PG antigens and in controlling oral microorganisms. |
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Trindade, Soraya Castro6732081357299415950544http://lattes.cnpq.br/7255547805484293Barreto Neto, Laerte Oliveira2019-11-13T22:09:49Z2019-09-03BARRETO NETO, Laerte Oliveira. Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite. 2019. 135 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2019.http://tede2.uefs.br:8080/handle/tede/927The use of herbal medicines has become a valuable resource in its treatment of oral disorders such as fungal and bacterial infections. One of the most prevalent infectious and inflammatory diseases in the mouth is periodontitis, which if left untreated, can lead to tooth loss. This study evaluated the immunomodulatory and antimicrobial potential of Lippia alnifolia Mart & Schauer (LA) and Vatairea macrocarpa (Benth.) Ducke (VM) leaf extract. Immunological evaluation was performed by immunoassay after culturing peripheral blood cells of individuals with and without periodontitis with the plant extract and the periodontopathogen Porphyromonas gingivalis (PG) extract. Antimicrobial activity was tested with minimal inhibitory concentration and disk diffusion assay. LA extract inhibited the production of IL-13, IL-6 and IFN-γ, both in relation to the basal concentration produced by cells and the concentration produced by cells co-cultured with PG extract. Co-cultivation of MV with PG promoted a reduction in IL-1β, IL-13, IL-6, IFN-γ, IL-10 and IL-17 levels. The LA extract showed bacteriostatic activity for Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella choleraesuis, fungistatic for Candida albicans and fungicide for Candida glabrata. VM showed bactericidal activity for Micrococcus luteus species and bacteriostatic other bacteria, as well as fungistatic activity for Candida albicans and fungicide for Candida glabrata. LA and VM extracts are promising in regulating cytokine production in PG antigens and in controlling oral microorganisms.O uso de fitoterápicos tem se tornado um recurso valioso no seu tratamento de afecções bucais, como infecções fúngicas e bacterianas. Uma das doenças infecciosas e inflamatórias mais prevalentes na boca é a periodontite, que se não tratada, pode levar à perda dentária. Este estudo avaliou o potencial imunomodulador e antimicrobiano do extrato das folhas de Lippia alnifolia Mart. & Schauer (LA) e Vatairea macrocarpa (Benth.)Ducke (VM). A avaliação imunológica foi realizada por imunoensaio, após cultivo das células do sangue periférico de indivíduos com e sem periodontite com o extrato da planta e o extrato do periodontopatógeno Porphyromonas gingivalis (PG). A atividade antimicrobiana foi testada com concentração inibitória mínima e ensaio de difusão em disco. O extrato de LA inibiu a produção de IL-13, IL-6 e IFN-γ, tanto em relação com a concentração basal produzida pelas células, quanto com a concentração produzida pelas células co-cultivadas com o extrato de PG. O co-cultivo de VM com PG promoveu uma redução nos níveis de IL-1β, IL-13, IL-6, IFN- γ, IL-10 e IL-17. O extrato de LA apresentou atividade bacteriostática para Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus e Salmonella choleraesuis, fungistática para Candida albicans e fungicida para Candida glabrata. VM apresentou atividade bactericida para a espécie Micrococcus luteus e bacteriostática demais bactérias, bem como atividade fungistática para Candida albicans e fungicida para Candida glabrata. Os extratos de LA e VM são promissores na regulação da produção de citocinas em a antígenos de PG e no controle de microrganismos bucais.Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2019-11-13T22:09:49Z No. of bitstreams: 1 Tese final Laerte.pdf: 4730378 bytes, checksum: a2c34124e294d4bd3f8c259f82b37cc3 (MD5)Made available in DSpace on 2019-11-13T22:09:49Z (GMT). No. of bitstreams: 1 Tese final Laerte.pdf: 4730378 bytes, checksum: a2c34124e294d4bd3f8c259f82b37cc3 (MD5) Previous issue date: 2019-09-03application/pdfporUniversidade Estadual de Feira de SantanaDoutorado Acadêmico em BiotecnologiaUEFSBrasilDEPARTAMENTO DE CIÊNCIAS BIOLÓGICASCitocinasDoenças PeriodontaisPlantas MedicinaisAnti-InfecciososCytokinesPeriodontal DiseasesPlants MedicinalAnti-infective agentsCIENCIAS BIOLOGICAS::BIOLOGIA GERALCIENCIAS BIOLOGICASEfeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontiteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-68152692297897915436006006006005026123383450589282-1634559385931244697-3439178843068202161info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEFSinstname:Universidade Estadual de Feira de Santana (UEFS)instacron:UEFSORIGINALTese final Laerte.pdfTese final Laerte.pdfapplication/pdf4730378http://tede2.uefs.br:8080/bitstream/tede/927/2/Tese+final+Laerte.pdfa2c34124e294d4bd3f8c259f82b37cc3MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://tede2.uefs.br:8080/bitstream/tede/927/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/9272019-11-13 19:09:49.469oai:tede2.uefs.br:8080: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.uefs.br:8080/PUBhttp://tede2.uefs.br:8080/oai/requestbcuefs@uefs.br|| bcref@uefs.br||bcuefs@uefs.bropendoar:2019-11-13T22:09:49Biblioteca Digital de Teses e Dissertações da UEFS - Universidade Estadual de Feira de Santana (UEFS)false |
dc.title.por.fl_str_mv |
Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite |
title |
Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite |
spellingShingle |
Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite Barreto Neto, Laerte Oliveira Citocinas Doenças Periodontais Plantas Medicinais Anti-Infecciosos Cytokines Periodontal Diseases Plants Medicinal Anti-infective agents CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
title_short |
Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite |
title_full |
Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite |
title_fullStr |
Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite |
title_full_unstemmed |
Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite |
title_sort |
Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite |
author |
Barreto Neto, Laerte Oliveira |
author_facet |
Barreto Neto, Laerte Oliveira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Trindade, Soraya Castro |
dc.contributor.advisor1ID.fl_str_mv |
67320813572 |
dc.contributor.authorID.fl_str_mv |
99415950544 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7255547805484293 |
dc.contributor.author.fl_str_mv |
Barreto Neto, Laerte Oliveira |
contributor_str_mv |
Trindade, Soraya Castro |
dc.subject.por.fl_str_mv |
Citocinas Doenças Periodontais Plantas Medicinais Anti-Infecciosos |
topic |
Citocinas Doenças Periodontais Plantas Medicinais Anti-Infecciosos Cytokines Periodontal Diseases Plants Medicinal Anti-infective agents CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Cytokines Periodontal Diseases Plants Medicinal Anti-infective agents |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL CIENCIAS BIOLOGICAS |
description |
The use of herbal medicines has become a valuable resource in its treatment of oral disorders such as fungal and bacterial infections. One of the most prevalent infectious and inflammatory diseases in the mouth is periodontitis, which if left untreated, can lead to tooth loss. This study evaluated the immunomodulatory and antimicrobial potential of Lippia alnifolia Mart & Schauer (LA) and Vatairea macrocarpa (Benth.) Ducke (VM) leaf extract. Immunological evaluation was performed by immunoassay after culturing peripheral blood cells of individuals with and without periodontitis with the plant extract and the periodontopathogen Porphyromonas gingivalis (PG) extract. Antimicrobial activity was tested with minimal inhibitory concentration and disk diffusion assay. LA extract inhibited the production of IL-13, IL-6 and IFN-γ, both in relation to the basal concentration produced by cells and the concentration produced by cells co-cultured with PG extract. Co-cultivation of MV with PG promoted a reduction in IL-1β, IL-13, IL-6, IFN-γ, IL-10 and IL-17 levels. The LA extract showed bacteriostatic activity for Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella choleraesuis, fungistatic for Candida albicans and fungicide for Candida glabrata. VM showed bactericidal activity for Micrococcus luteus species and bacteriostatic other bacteria, as well as fungistatic activity for Candida albicans and fungicide for Candida glabrata. LA and VM extracts are promising in regulating cytokine production in PG antigens and in controlling oral microorganisms. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-11-13T22:09:49Z |
dc.date.issued.fl_str_mv |
2019-09-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARRETO NETO, Laerte Oliveira. Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite. 2019. 135 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2019. |
dc.identifier.uri.fl_str_mv |
http://tede2.uefs.br:8080/handle/tede/927 |
identifier_str_mv |
BARRETO NETO, Laerte Oliveira. Efeito imunomodulador e antimicrobiano dos extratos de Lippia alnifolia e Vatairea macrocarpa na periodontite. 2019. 135 f. Tese (Doutorado Acadêmico em Biotecnologia)- Universidade Estadual de Feira de Santana, Feira de Santana, 2019. |
url |
http://tede2.uefs.br:8080/handle/tede/927 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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600 600 600 600 |
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5026123383450589282 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
dc.publisher.program.fl_str_mv |
Doutorado Acadêmico em Biotecnologia |
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UEFS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS |
publisher.none.fl_str_mv |
Universidade Estadual de Feira de Santana |
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