Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection

Detalhes bibliográficos
Autor(a) principal: Escobar, Taiane Acunha
Data de Publicação: 2020
Outros Autores: Döwich, Gabriela, Cantele, Letícia Carvalho, Góss, Geórgia Camargo, Porciúncula, Marcelo Lameiro, Leite, Carla Teixeira, Zuravski, Luísa, Duarte, Claudia Acosta, Lubeck, Irina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36172
Resumo: Leishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.
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spelling Applications of polymerase chain reaction for the detection of equine Leishmania sp. infectionAplicação da reação em cadeia da polimerase na detecção da infecção por Leishmania sp. em equinosLeishmania infantumDiagnostic methodsInfectionHorses.Leishmania infantumMétodos diagnósticosInfecçãoCavalos.Leishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.A leishmaniose é uma doença zoonótica negligenciada, causada por uma diversidade de espécies patogênicas de Leishmania. No Novo Mundo, especialmente no Brasil, a leishmaniose visceral é causada pelo protozoário Leishmania infantum. A infecção acomete várias espécies animais (cães, raposas, roedores, primatas, felinos, equinos) e humanos e o cão é o principal reservatório doméstico. Este estudo teve como objetivo utilizar a reação em cadeia da polimerase (PCR) para detectar a infecção por Leishmania sp. em cavalos que vivem em uma região endêmica para leishmaniose visceral canina (LVC). Amostras de DNA do sangue periférico de equinos foram utilizadas para extração de DNA e PCR. As amplificações foram realizadas utilizando-se marcadores para a região do DNA do cinetoplasto (kDNA), que são capazes de detectar diferentes espécies de Leishmania sp. e a região dos espaçadores internos transcritos (ITS) do DNA ribossomal para detecção da família Trypanosomatidae. Dentre os 192 animais testados, 75 (39%) amostras foram positivas no PCR, 21 amostras foram positivas para PCR kDNA e 63 para os genes ITS, ITS1 e ITS2. O sequenciamento dos amplicons permitiu detectar L. infantum em amostras de sangue periférico de equinos. Este foi o primeiro relato de infecção por L. infantum no sul do Brasil na espécie equina. Os resultados demonstram que, assim como nos países europeus, a infecção foi detectada em equinos após ter sido identificada em seres humanos e cães, o que indica a urgência em acompanhar a investigação da infecção nessa espécie animal.UEL2020-01-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3617210.5433/1679-0359.2020v41n1p199Semina: Ciências Agrárias; Vol. 41 No. 1 (2020); 199-212Semina: Ciências Agrárias; v. 41 n. 1 (2020); 199-2121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36172/26403Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessEscobar, Taiane AcunhaDöwich, GabrielaCantele, Letícia CarvalhoGóss, Geórgia CamargoPorciúncula, Marcelo LameiroLeite, Carla TeixeiraZuravski, LuísaDuarte, Claudia AcostaLubeck, Irina2022-10-10T14:42:19Zoai:ojs.pkp.sfu.ca:article/36172Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T14:42:19Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection
Aplicação da reação em cadeia da polimerase na detecção da infecção por Leishmania sp. em equinos
title Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection
spellingShingle Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection
Escobar, Taiane Acunha
Leishmania infantum
Diagnostic methods
Infection
Horses.
Leishmania infantum
Métodos diagnósticos
Infecção
Cavalos.
title_short Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection
title_full Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection
title_fullStr Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection
title_full_unstemmed Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection
title_sort Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection
author Escobar, Taiane Acunha
author_facet Escobar, Taiane Acunha
Döwich, Gabriela
Cantele, Letícia Carvalho
Góss, Geórgia Camargo
Porciúncula, Marcelo Lameiro
Leite, Carla Teixeira
Zuravski, Luísa
Duarte, Claudia Acosta
Lubeck, Irina
author_role author
author2 Döwich, Gabriela
Cantele, Letícia Carvalho
Góss, Geórgia Camargo
Porciúncula, Marcelo Lameiro
Leite, Carla Teixeira
Zuravski, Luísa
Duarte, Claudia Acosta
Lubeck, Irina
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Escobar, Taiane Acunha
Döwich, Gabriela
Cantele, Letícia Carvalho
Góss, Geórgia Camargo
Porciúncula, Marcelo Lameiro
Leite, Carla Teixeira
Zuravski, Luísa
Duarte, Claudia Acosta
Lubeck, Irina
dc.subject.por.fl_str_mv Leishmania infantum
Diagnostic methods
Infection
Horses.
Leishmania infantum
Métodos diagnósticos
Infecção
Cavalos.
topic Leishmania infantum
Diagnostic methods
Infection
Horses.
Leishmania infantum
Métodos diagnósticos
Infecção
Cavalos.
description Leishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36172
10.5433/1679-0359.2020v41n1p199
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36172
identifier_str_mv 10.5433/1679-0359.2020v41n1p199
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36172/26403
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 1 (2020); 199-212
Semina: Ciências Agrárias; v. 41 n. 1 (2020); 199-212
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
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instname_str Universidade Estadual de Londrina (UEL)
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institution UEL
reponame_str Semina. Ciências Agrárias (Online)
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repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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