Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae)
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2187 |
Resumo: | One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets. |
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Semina. Ciências Agrárias (Online) |
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Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae)Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismosTissue cultureCulture mediumOrchidFungiBacteria.Cultura de tecidosMeio de culturaOrquídeaFungosBactérias.One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets.Um dos maiores problemas na produção de mudas in vitro, é a contaminação por fungos e bactérias do meio de cultura durante as etapas de micropropagação, mesmo havendo todos os cuidados de assepsia. O presente trabalho teve por objetivo avaliar o efeito de fungicidas e germicida no crescimento vegetativo e enraizamento in vitro da orquídea Oncidium varicosum Lindl. As flores foram polinizadas artificialmente e as sementes foram germinadas no meio MS modificado com a metade da concentração dos macronutrientes. As plântulas obtidas foram subcultivadas no mesmo meio de germinação, adicionando-se os fungicidas azoxystrobin, benomyl, chlorothalonil nas concentrações: 0,1 g.L-1; 0,2 g.L-1 ; 0,4 g.L-1; 0,6 g .L-1 e 0,8 g.L-1 O germicida hipoclorito de sódio com 10% de cloro ativo foi adicionado ao meio nutritivo nas concentrações: 0,5 mL. L-1; 0,75 mL .L-1 ; 1,00 mL .L-1 ; 1,25 mL. L-1 e 1,50 mL. L-1 . O delineamento experimental foi o inteiramente casualizado, com dez repetições por tratamento e cinco plântulas por frasco. O tratamento contendo hipoclorito de sódio 1,5 mL . L-1 mostrou-se o menos tóxico, pois não afetou o desenvolvimento vegetativo e o enraizamento in vitro de plântulas de Oncidium varicosum.UEL2003-05-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/218710.5433/1679-0359.2003v24n2p273Semina: Ciências Agrárias; Vol. 24 No. 2 (2003); 273-276Semina: Ciências Agrárias; v. 24 n. 2 (2003); 273-2761679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2187/1877Oda, Melissa LombardiFaria, Ricardo Tadeu deFonseca, Inês Cristina BatistaSilva, Geraldo Lopesinfo:eu-repo/semantics/openAccess2009-05-26T19:03:27Zoai:ojs.pkp.sfu.ca:article/2187Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-05-26T19:03:27Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) Avaliação da fitotoxicidade de fungicidas e germicida na propagação in vitro de Oncidium varicosum Lindl. (Orchidaceae) para o controle de microorganismos |
title |
Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) |
spellingShingle |
Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) Oda, Melissa Lombardi Tissue culture Culture medium Orchid Fungi Bacteria. Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. |
title_short |
Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) |
title_full |
Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) |
title_fullStr |
Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) |
title_full_unstemmed |
Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) |
title_sort |
Fungicide and germicide on contamination escaping in the in vitro propagation of Oncidium varicosum Lindl. (Orchidaceae) |
author |
Oda, Melissa Lombardi |
author_facet |
Oda, Melissa Lombardi Faria, Ricardo Tadeu de Fonseca, Inês Cristina Batista Silva, Geraldo Lopes |
author_role |
author |
author2 |
Faria, Ricardo Tadeu de Fonseca, Inês Cristina Batista Silva, Geraldo Lopes |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oda, Melissa Lombardi Faria, Ricardo Tadeu de Fonseca, Inês Cristina Batista Silva, Geraldo Lopes |
dc.subject.por.fl_str_mv |
Tissue culture Culture medium Orchid Fungi Bacteria. Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. |
topic |
Tissue culture Culture medium Orchid Fungi Bacteria. Cultura de tecidos Meio de cultura Orquídea Fungos Bactérias. |
description |
One of the greatest problem of in vitro production of seedling is the contamination of the culture medium during the micropropagation stages. This study was carried out to investigate the effect of fungicides and germicide on the plant growth and rooting, degree of phytotoxicity and control of contamination during in vitro cultivation of O. varicosum orchid. The flowers were pollinated artificially and the capsules seeds were sowed in MS culture medium modified with half of the macronutrients. The seedlings were transferred to the same culture medium uded for germination, added with the fungicides azoxystrobin, benomyl, chlorothalonil ( 0 g.L-1 , 0.1 g.L-1 ; 0.2 g.L-1 ; 0.4 g.L-1 ; 0.6 g.L-1 and 0.8 g.L-1) or sodium hypochloride with 10% active chloride ( 0 mL. L-1 , 0,5 mL.L-1 ; 0,75 mL. L-1 ; 1,00 mL. L-1 ; 1,25 mL. L-1 ; 1,50 mL. L-1). A complete randomized block design was used with ten replications per treatment and five plantlets per flask. The treatment containing 1.5 mL L-1 sodium hypochloride was the best in preventing contamination by microorganisms and did not cause any apparent harm development and in vitro rooting of Oncidium varicosum plantlets. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-05-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2187 10.5433/1679-0359.2003v24n2p273 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2187 |
identifier_str_mv |
10.5433/1679-0359.2003v24n2p273 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2187/1877 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 24 No. 2 (2003); 273-276 Semina: Ciências Agrárias; v. 24 n. 2 (2003); 273-276 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306057143549952 |