In vitro growth of Oncidium baueri (Orchidaceae) at different macronutrients and sucrose concentrations

• Autor(a) principal: Sorace, Mauren
• Data de Publicação: 2008
• Outros Autores: Faria, Ricardo Tadeu de, Damasceno Júnior, Clério Valentin, Gomes, Gisely Paula Gomes, Barbosa, Cristiane Muniz, Vieira, Fabíola Giovanna Nesello, Silva, Geraldo Lopes da, Takahashi, Lúcia Sadayo Assari, Schnitzer, Jenniffer Aparecida
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Semina. Ciências Agrárias (Online)
• Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2691
• topic: Carbohydrate; Orchids; Tissue culture.; Carboidrato; Orquídeas; Cultura de tecido.

The  propagation  in  vitro  is  an  important method  for  orchids  reproduction,  because  the seeds  don’t  have endosperm and present low germination. The sugar is a important component in medium culture, serving as a  source of carbon and energy. The objective of  this  study was  to evaluate  the growth  in vitro of Oncidium baueri  (Orchidaceae)  in different macronutrients and  sucrose concentrations. The treatments consisted: two formulations of the macronutrientes of the MS medium, in complete MS and MS modifed medium with half of  the regular concentration of macronutrients, and different sucrose concentrations (30 gL-1, 40 gL-1e 60 gL-1), at pH 5,8. A randomized design with eight replications was used as statistical model and a fask with  twenty plants composed each plot. Data were submitted  to analysis of variance, complemented by  the Tukey  test at 5% of signifcance. The following variables were assessed six months after the beguining of the experiment: plant height, root length; number of roots, number of buds, dry mass and total fresh mass. The treatment that showed the best results was 40 gL-1 sucrose  in a modifed MS medium with half of  regular concentration of macronutrients,  for vegetative development and rooting of Oncidium baueri orchid.