Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36689 |
Resumo: | The early blight (caused by the fungus Alternaria solani) results in significant damage to the tomato crop, directly affecting productivity. An alternative to the frequent use of pesticides is the use of essential oils, which can act in defense against phytopathogens. The objective of this work was to evaluate the toxic activity in vitro of the bergamot orange (Citrus aurantium ssp. bergamia) essential oil against A. solani, the control of the early blight, and the activity of defense enzymes in tomatoes treated with this oil and inoculated with A. solani. Mycelial discs of A. solani were added to dishes with V8 culture media to which essential oil at concentrations of 0, 500, 1000, 1500, 2000, and 2500 µL L-1, in addition to a standard fungicide treatment (azoxystrobin + difenoconazole, 200 + 125 g L-1, respectively) was added. The Petri dishes were incubated at 25 °C in the dark. Mycelial growth was evaluated daily for 19 days, when all treatments reached maximum growth. Sporulation analysis was performed thereafter. Tomato plants were treated with bergamot essential oil, 30 days after transplanting, in the concentrations and fungicide mentioned, in the second pair of leaves. After 72 hours, the pathogen was inoculated using a spray bottle, on treated leaves (second pair of leaves) and untreated leaves (third pair of leaves). The area under the disease progress curve (AUDPC) was calculated based on five severity assessments. The activity of peroxidase (POX), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) was evaluated in leaves treated with the concentration of 2500 µL L-1 of essential oil. The concentration of 2500 ?L L-1 reduced mycelial growth and sporulation of the pathogen by 68.15% and 29.48%, respectively. In treatments with application of essential oil, lower AUDPC was observed for a concentration of 2500 ?L L-1, which was statistically similar to that observed for fungicide application, both in treated and untreated leaves. A greater activity of PPO, POX, and PAL was found locally and systemically, both in the second and third leaves, at concentration of 2500 ?L L-1. The essential oil of bergamot can be an alternative for the control of early blight in tomato. |
id |
UEL-11_c9e76d1acc2ea30030b78f728f5f707a |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/36689 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orangeAtividade antifúngica contra Alternaria solani e controle da pinta preta do tomateiro por óleo essencial de bergamotaCitrus aurantiumAlternative controlResistance induction.Citrus aurantiumControle alternativoIndução de resistência.The early blight (caused by the fungus Alternaria solani) results in significant damage to the tomato crop, directly affecting productivity. An alternative to the frequent use of pesticides is the use of essential oils, which can act in defense against phytopathogens. The objective of this work was to evaluate the toxic activity in vitro of the bergamot orange (Citrus aurantium ssp. bergamia) essential oil against A. solani, the control of the early blight, and the activity of defense enzymes in tomatoes treated with this oil and inoculated with A. solani. Mycelial discs of A. solani were added to dishes with V8 culture media to which essential oil at concentrations of 0, 500, 1000, 1500, 2000, and 2500 µL L-1, in addition to a standard fungicide treatment (azoxystrobin + difenoconazole, 200 + 125 g L-1, respectively) was added. The Petri dishes were incubated at 25 °C in the dark. Mycelial growth was evaluated daily for 19 days, when all treatments reached maximum growth. Sporulation analysis was performed thereafter. Tomato plants were treated with bergamot essential oil, 30 days after transplanting, in the concentrations and fungicide mentioned, in the second pair of leaves. After 72 hours, the pathogen was inoculated using a spray bottle, on treated leaves (second pair of leaves) and untreated leaves (third pair of leaves). The area under the disease progress curve (AUDPC) was calculated based on five severity assessments. The activity of peroxidase (POX), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) was evaluated in leaves treated with the concentration of 2500 µL L-1 of essential oil. The concentration of 2500 ?L L-1 reduced mycelial growth and sporulation of the pathogen by 68.15% and 29.48%, respectively. In treatments with application of essential oil, lower AUDPC was observed for a concentration of 2500 ?L L-1, which was statistically similar to that observed for fungicide application, both in treated and untreated leaves. A greater activity of PPO, POX, and PAL was found locally and systemically, both in the second and third leaves, at concentration of 2500 ?L L-1. The essential oil of bergamot can be an alternative for the control of early blight in tomato.A pinta preta (causada pelo fungo Alternaria solani) causa prejuízos significativos na cultura do tomateiro, afetando diretamente a produtividade. Uma alternativa ao uso frequente de agrotóxico é a utilização de óleos essenciais, que podem atuar na defesa contra fitopatógenos. O objetivo deste trabalho foi avaliar a atividade tóxica in vitro do óleo essencial de bergamota (Citrus aurantium subsp. bergamia) contra A. solani, o controle da pinta preta e a atividade de enzimas de defesa em tomateiros tratados com esse óleo e inoculados com A. solani. Discos miceliais de A. solani foram adicionados em placas com meio de cultura V8 adicionado de óleo essencial nas concentrações de 0, 500, 1000, 1500, 2000 e 2500 µL L-1, além de um tratamento padrão com fungicida (azoxistrobina + difenoconazol, 200 + 125 g L-1 respectivamente). As placas de Petri foram incubadas a 25 °C no escuro. O crescimento micelial foi avaliado diariamente, por 19 dias, quando todos os tratamentos atingiram o máximo crescimento, sendo então feita a análise da esporulação. Aos 30 dias após o transplantio, o segundo par de folhas de plantas de tomate foram tratadas com óleo essencial de bergamota e de fungicida nas concentrações de óleo citados. Após 72 horas o patógeno foi inoculado com borrifador, nas folhas tratadas (segundo par de folhas) e nas não tratadas (terceiro par de folhas). A área abaixo da curva de progresso da doença (AACPD) foi calculada com base em cinco avaliações de severidade. A atividade de peroxidase (POX), polifenoloxidase (PPO) e fenilalaninaamônia-liase (PAL) foi avaliada em folhas tratadas com a concentração de 2500 µL L-1 de óleo essencial. A concentração de 2500 ?L L-1 reduziu o crescimento micelial e a esporulação do patógeno, em 68,15% e 29,48%, respectivamente. Nos tratamentos com aplicação de óleo essencial, a menor AACPD foi observada para a concentração de 2500 ?L L-1, sendo semelhante estatisticamente ao observado para a aplicação de fungicida, tanto nas folhas tratadas quanto nas não tratadas. Foi constatada uma maior atividade de PPO, POX e PAL de maneira local e sistêmica, tanto no 2° quanto no 3° par de folhas, na concentração de 2500 ?L L-1. O óleo essencial de bergamota pode ser uma alternativa no controle da pinta preta do tomateiro.UEL2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3668910.5433/1679-0359.2020v41n5supl1p1861Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 1861-1874Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 1861-18741679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36689/27765Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessHendges, CamilaStangarlin, José RenatoNozaki, Márcia de HolandaLorenzetti, EloisaKuhn, Odair José2022-10-07T12:35:12Zoai:ojs.pkp.sfu.ca:article/36689Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T12:35:12Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange Atividade antifúngica contra Alternaria solani e controle da pinta preta do tomateiro por óleo essencial de bergamota |
title |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange |
spellingShingle |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange Hendges, Camila Citrus aurantium Alternative control Resistance induction. Citrus aurantium Controle alternativo Indução de resistência. |
title_short |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange |
title_full |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange |
title_fullStr |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange |
title_full_unstemmed |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange |
title_sort |
Antifungal activity against Alternaria solani and control of early blight in tomato by essential oil of bergamot orange |
author |
Hendges, Camila |
author_facet |
Hendges, Camila Stangarlin, José Renato Nozaki, Márcia de Holanda Lorenzetti, Eloisa Kuhn, Odair José |
author_role |
author |
author2 |
Stangarlin, José Renato Nozaki, Márcia de Holanda Lorenzetti, Eloisa Kuhn, Odair José |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hendges, Camila Stangarlin, José Renato Nozaki, Márcia de Holanda Lorenzetti, Eloisa Kuhn, Odair José |
dc.subject.por.fl_str_mv |
Citrus aurantium Alternative control Resistance induction. Citrus aurantium Controle alternativo Indução de resistência. |
topic |
Citrus aurantium Alternative control Resistance induction. Citrus aurantium Controle alternativo Indução de resistência. |
description |
The early blight (caused by the fungus Alternaria solani) results in significant damage to the tomato crop, directly affecting productivity. An alternative to the frequent use of pesticides is the use of essential oils, which can act in defense against phytopathogens. The objective of this work was to evaluate the toxic activity in vitro of the bergamot orange (Citrus aurantium ssp. bergamia) essential oil against A. solani, the control of the early blight, and the activity of defense enzymes in tomatoes treated with this oil and inoculated with A. solani. Mycelial discs of A. solani were added to dishes with V8 culture media to which essential oil at concentrations of 0, 500, 1000, 1500, 2000, and 2500 µL L-1, in addition to a standard fungicide treatment (azoxystrobin + difenoconazole, 200 + 125 g L-1, respectively) was added. The Petri dishes were incubated at 25 °C in the dark. Mycelial growth was evaluated daily for 19 days, when all treatments reached maximum growth. Sporulation analysis was performed thereafter. Tomato plants were treated with bergamot essential oil, 30 days after transplanting, in the concentrations and fungicide mentioned, in the second pair of leaves. After 72 hours, the pathogen was inoculated using a spray bottle, on treated leaves (second pair of leaves) and untreated leaves (third pair of leaves). The area under the disease progress curve (AUDPC) was calculated based on five severity assessments. The activity of peroxidase (POX), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) was evaluated in leaves treated with the concentration of 2500 µL L-1 of essential oil. The concentration of 2500 ?L L-1 reduced mycelial growth and sporulation of the pathogen by 68.15% and 29.48%, respectively. In treatments with application of essential oil, lower AUDPC was observed for a concentration of 2500 ?L L-1, which was statistically similar to that observed for fungicide application, both in treated and untreated leaves. A greater activity of PPO, POX, and PAL was found locally and systemically, both in the second and third leaves, at concentration of 2500 ?L L-1. The essential oil of bergamot can be an alternative for the control of early blight in tomato. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36689 10.5433/1679-0359.2020v41n5supl1p1861 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36689 |
identifier_str_mv |
10.5433/1679-0359.2020v41n5supl1p1861 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/36689/27765 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 1861-1874 Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 1861-1874 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306081603682304 |