Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Daniele Silva
Data de Publicação: 2012
Outros Autores: Melo, Daniela Benevides, Guimarães, Alaíse Gil, Velozo, Eudes Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496
Resumo: Microorganisms can be transmitted by food causing diseases in humans. The antibiotics commonly used in treatment of these diseases have shown little or no effect, and in view of the resistance that many microorganisms have acquired. This study evaluated the essential oil leaves of Rosmarinus officinalis L. as a modulator of resistance bacterial drug. The essential oil was obtained by hydrodistillation in a Clevenger apparatus for 3 hours. We tested four strains of E.coli resistant ampicillin (AMP) and tetracycline (TET) and four strains of Salmonella spp. Resistant to nitrofurantoin (NIT). The strains in 0.5 MacFarland scale suspension were inoculated on Mueller Hinton agar, then soaked antibiotic disks with 10 and 20?L oil pure rosemary were placed on the plates. After 24h/37 º C were measured halos around the discs. All strains tested showed susceptibility to the combined action of essential oil with antibiotics tested. The results indicate that the use of promising rosemary essential oil in combination with antibiotics to combat pathogenic bacteria.
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spelling Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistanceAvaliação do óleo essencial de alecrim (Rosmarinus officinalis L.) como modulador da resistência bacterianaEssential oilRosemaryBacterial resistance.5.07.01.00-2Óleo essencialAlecrimResistência bacteriana.5.07.01.00-2Microorganisms can be transmitted by food causing diseases in humans. The antibiotics commonly used in treatment of these diseases have shown little or no effect, and in view of the resistance that many microorganisms have acquired. This study evaluated the essential oil leaves of Rosmarinus officinalis L. as a modulator of resistance bacterial drug. The essential oil was obtained by hydrodistillation in a Clevenger apparatus for 3 hours. We tested four strains of E.coli resistant ampicillin (AMP) and tetracycline (TET) and four strains of Salmonella spp. Resistant to nitrofurantoin (NIT). The strains in 0.5 MacFarland scale suspension were inoculated on Mueller Hinton agar, then soaked antibiotic disks with 10 and 20?L oil pure rosemary were placed on the plates. After 24h/37 º C were measured halos around the discs. All strains tested showed susceptibility to the combined action of essential oil with antibiotics tested. The results indicate that the use of promising rosemary essential oil in combination with antibiotics to combat pathogenic bacteria.Diversos microrganismos podem ser veiculados por alimentos causando doenças nos seres humanos. Os antibióticos comumente utilizados no tratamento dessas doenças têm apresentado baixo ou nenhum efeito, tendo em vista à resistência que muitos microrganismos têm adquirido. Diante deste quadro, o objetivo desta pesquisa foi avaliar o óleo essencial das folhas de Rosmarinus officinalis L. como modulador da resistência bacteriana a drogas. O óleo essencial foi obtido através de hidrodestilação em aparelho de Clevenger por 3h. Foram testadas 4 cepas de E.coli resistentes a Amplicilina (AMP) e a Tetraciclina (TET) e 4 cepas de Salmonella spp. resistentes a Nitrofurantoína (NIT). As cepas em suspensão escala MacFarland 0,5 foram inoculadas em agar Mueller Hinton, em seguida os discos dos antibióticos embebidos com 10 e 20?L do óleo de alecrim puro foram dispostos sobre as placas. Após 24h/37ºC foram medidos os halos ao redor dos discos. Todas as cepas avaliadas apresentaram susceptibilidade à ação combinada do óleo essencial com os antibióticos testados. Os resultados obtidos indicam ser promissora a utilização de óleo essencial de alecrim em associação com antibióticos no combate a bactérias patogênicas.UEL2012-05-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/749610.5433/1679-0359.2012v33n2p687Semina: Ciências Agrárias; Vol. 33 No. 2 (2012); 687-696Semina: Ciências Agrárias; v. 33 n. 2 (2012); 687-6961679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496/10452Ribeiro, Daniele SilvaMelo, Daniela BenevidesGuimarães, Alaíse GilVelozo, Eudes Silvainfo:eu-repo/semantics/openAccess2015-11-19T18:37:37Zoai:ojs.pkp.sfu.ca:article/7496Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:37:37Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
Avaliação do óleo essencial de alecrim (Rosmarinus officinalis L.) como modulador da resistência bacteriana
title Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
spellingShingle Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
Ribeiro, Daniele Silva
Essential oil
Rosemary
Bacterial resistance.
5.07.01.00-2
Óleo essencial
Alecrim
Resistência bacteriana.
5.07.01.00-2
title_short Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
title_full Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
title_fullStr Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
title_full_unstemmed Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
title_sort Evaluation of the rosemary essential oil (Rosmarinus officinalis L.) as modulator of bacterial resistance
author Ribeiro, Daniele Silva
author_facet Ribeiro, Daniele Silva
Melo, Daniela Benevides
Guimarães, Alaíse Gil
Velozo, Eudes Silva
author_role author
author2 Melo, Daniela Benevides
Guimarães, Alaíse Gil
Velozo, Eudes Silva
author2_role author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Daniele Silva
Melo, Daniela Benevides
Guimarães, Alaíse Gil
Velozo, Eudes Silva
dc.subject.por.fl_str_mv Essential oil
Rosemary
Bacterial resistance.
5.07.01.00-2
Óleo essencial
Alecrim
Resistência bacteriana.
5.07.01.00-2
topic Essential oil
Rosemary
Bacterial resistance.
5.07.01.00-2
Óleo essencial
Alecrim
Resistência bacteriana.
5.07.01.00-2
description Microorganisms can be transmitted by food causing diseases in humans. The antibiotics commonly used in treatment of these diseases have shown little or no effect, and in view of the resistance that many microorganisms have acquired. This study evaluated the essential oil leaves of Rosmarinus officinalis L. as a modulator of resistance bacterial drug. The essential oil was obtained by hydrodistillation in a Clevenger apparatus for 3 hours. We tested four strains of E.coli resistant ampicillin (AMP) and tetracycline (TET) and four strains of Salmonella spp. Resistant to nitrofurantoin (NIT). The strains in 0.5 MacFarland scale suspension were inoculated on Mueller Hinton agar, then soaked antibiotic disks with 10 and 20?L oil pure rosemary were placed on the plates. After 24h/37 º C were measured halos around the discs. All strains tested showed susceptibility to the combined action of essential oil with antibiotics tested. The results indicate that the use of promising rosemary essential oil in combination with antibiotics to combat pathogenic bacteria.
publishDate 2012
dc.date.none.fl_str_mv 2012-05-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496
10.5433/1679-0359.2012v33n2p687
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496
identifier_str_mv 10.5433/1679-0359.2012v33n2p687
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/7496/10452
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 33 No. 2 (2012); 687-696
Semina: Ciências Agrárias; v. 33 n. 2 (2012); 687-696
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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