Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen

Detalhes bibliográficos
Autor(a) principal: Silva, Fernando Evaristo da
Data de Publicação: 2020
Outros Autores: Carvalho, Jaqueline Candido, Dell'Aqua, Camila de Paula Freitas, Papa, Frederico Ozanam, Henry, Marc Roger Jean Marie, Oba, Eunice, Ferreira, João Carlos Pinheiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38274
Resumo: The use of cooled semen in artificial insemination operations results in higher pregnancy rates than the use of frozen semen. This result seems to be related to the more severe damage triggered by the freezing process than that observed during refrigeration. Due to its ability to bind to sperm-binding proteins and calcium ions, sodium caseinate has been studied as a substance capable of preventing early sperm capacitation, a significant cause of the decreased pregnancy rate resulting from the use of frozen semen. The first objective of this study was to evaluate whether a commercial egg yolk diluent developed for frozen bovine semen could be used for buffalo semen cryopreservation; the second objective was to investigate the effect of this diluent in combination with sodium caseinate during the procedures of buffalo sperm cryopreservation using flow cytometry and computer-assisted sperm analysis. In the first part of the study, comparing the results of spermatic kinetics and plasma and acrosomal membrane integrity, it was observed that the freezing process resulted in more cell damage than the cooling process. In the second part of the study, no effects of the addition of sodium caseinate to the egg yolk diluent were observed. From the results of the present study, it was possible to conclude that the egg yolk-based diluent was suitable for buffalo semen cryopreservation and that the addition of sodium caseinate did not decrease the harmful effects related to seminal cryopreservation.
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spelling Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semenEfeito da adição de caseinato de sódio sobre a viabilidade do sêmen bubalino criopreservadoBSPBuffaloCaseinCryopreservationSperm.BSPBúfaloCaseínaCriopreservaçãoEspermatozoide.The use of cooled semen in artificial insemination operations results in higher pregnancy rates than the use of frozen semen. This result seems to be related to the more severe damage triggered by the freezing process than that observed during refrigeration. Due to its ability to bind to sperm-binding proteins and calcium ions, sodium caseinate has been studied as a substance capable of preventing early sperm capacitation, a significant cause of the decreased pregnancy rate resulting from the use of frozen semen. The first objective of this study was to evaluate whether a commercial egg yolk diluent developed for frozen bovine semen could be used for buffalo semen cryopreservation; the second objective was to investigate the effect of this diluent in combination with sodium caseinate during the procedures of buffalo sperm cryopreservation using flow cytometry and computer-assisted sperm analysis. In the first part of the study, comparing the results of spermatic kinetics and plasma and acrosomal membrane integrity, it was observed that the freezing process resulted in more cell damage than the cooling process. In the second part of the study, no effects of the addition of sodium caseinate to the egg yolk diluent were observed. From the results of the present study, it was possible to conclude that the egg yolk-based diluent was suitable for buffalo semen cryopreservation and that the addition of sodium caseinate did not decrease the harmful effects related to seminal cryopreservation.O uso de sêmen resfriado em operações de inseminação artificial resulta em taxas de prenhez mais altas do que o uso de sêmen congelado. Esse resultado parece estar relacionado aos danos mais severos desencadeados pelo processo de congelação, quando comparado com o de refrigeração. Devido à sua capacidade de se ligar às proteínas ligadoras de espermatozoides e íons cálcio, o caseinato de sódio foi estudado quanto a sua capacidade de prevenir a capacitação espermática precoce, uma causa significativa de diminuição da taxa de prenhez com o uso de sêmen congelado. O primeiro objetivo deste estudo foi avaliar se um diluidor comercial a base de gema de ovo, destinado à congelação de sêmen bovino, poderia ser usado para a criopreservação de sêmen bubalino; o segundo objetivo foi investigar o efeito da adição do caseinato de sódio ao diluidor estudado durante os procedimentos de criopreservação de espermatozoides bubalinos. Foram empregadas a citometria de fluxo e a análise computadorizada do movimento espermático como métodos de avaliação seminal. Na primeira fase do estudo, comparando-se os resultados da cinética espermática e da integridade das membranas plasmática e acrossomal, observou-se que o processo de congelação promoveu mais danos celulares que o processo de resfriamento. Na segunda fase do estudo, não foram observados efeitos benéficos da adição de caseinato de sódio ao diluente empregado. A partir dos resultados do presente estudo, foi possível concluir que o diluente à base de gema de ovo foi adequado para a criopreservação do sêmen de bubalino e que a adição do caseinato de sódio não diminuiu os efeitos deletérios desencadeados pelo processo de criopreservação seminal.UEL2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3827410.5433/1679-0359.2020v41n5supl1p2209Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2209-2218Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2209-22181679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38274/27845Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSilva, Fernando Evaristo daCarvalho, Jaqueline CandidoDell'Aqua, Camila de Paula FreitasPapa, Frederico OzanamHenry, Marc Roger Jean MarieOba, EuniceFerreira, João Carlos Pinheiro2022-10-07T13:42:59Zoai:ojs.pkp.sfu.ca:article/38274Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T13:42:59Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
Efeito da adição de caseinato de sódio sobre a viabilidade do sêmen bubalino criopreservado
title Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
spellingShingle Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
Silva, Fernando Evaristo da
BSP
Buffalo
Casein
Cryopreservation
Sperm.
BSP
Búfalo
Caseína
Criopreservação
Espermatozoide.
title_short Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
title_full Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
title_fullStr Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
title_full_unstemmed Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
title_sort Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen
author Silva, Fernando Evaristo da
author_facet Silva, Fernando Evaristo da
Carvalho, Jaqueline Candido
Dell'Aqua, Camila de Paula Freitas
Papa, Frederico Ozanam
Henry, Marc Roger Jean Marie
Oba, Eunice
Ferreira, João Carlos Pinheiro
author_role author
author2 Carvalho, Jaqueline Candido
Dell'Aqua, Camila de Paula Freitas
Papa, Frederico Ozanam
Henry, Marc Roger Jean Marie
Oba, Eunice
Ferreira, João Carlos Pinheiro
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Fernando Evaristo da
Carvalho, Jaqueline Candido
Dell'Aqua, Camila de Paula Freitas
Papa, Frederico Ozanam
Henry, Marc Roger Jean Marie
Oba, Eunice
Ferreira, João Carlos Pinheiro
dc.subject.por.fl_str_mv BSP
Buffalo
Casein
Cryopreservation
Sperm.
BSP
Búfalo
Caseína
Criopreservação
Espermatozoide.
topic BSP
Buffalo
Casein
Cryopreservation
Sperm.
BSP
Búfalo
Caseína
Criopreservação
Espermatozoide.
description The use of cooled semen in artificial insemination operations results in higher pregnancy rates than the use of frozen semen. This result seems to be related to the more severe damage triggered by the freezing process than that observed during refrigeration. Due to its ability to bind to sperm-binding proteins and calcium ions, sodium caseinate has been studied as a substance capable of preventing early sperm capacitation, a significant cause of the decreased pregnancy rate resulting from the use of frozen semen. The first objective of this study was to evaluate whether a commercial egg yolk diluent developed for frozen bovine semen could be used for buffalo semen cryopreservation; the second objective was to investigate the effect of this diluent in combination with sodium caseinate during the procedures of buffalo sperm cryopreservation using flow cytometry and computer-assisted sperm analysis. In the first part of the study, comparing the results of spermatic kinetics and plasma and acrosomal membrane integrity, it was observed that the freezing process resulted in more cell damage than the cooling process. In the second part of the study, no effects of the addition of sodium caseinate to the egg yolk diluent were observed. From the results of the present study, it was possible to conclude that the egg yolk-based diluent was suitable for buffalo semen cryopreservation and that the addition of sodium caseinate did not decrease the harmful effects related to seminal cryopreservation.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38274
10.5433/1679-0359.2020v41n5supl1p2209
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38274
identifier_str_mv 10.5433/1679-0359.2020v41n5supl1p2209
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38274/27845
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2209-2218
Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2209-2218
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306082268479488

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