Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability

Detalhes bibliográficos
Autor(a) principal: Magalhães Jr, Marcos Jorge
Data de Publicação: 2016
Outros Autores: Martins, Leonardo Franco, Senra, Renato Lima, Santos, Thaís Ferreira do, Okano, Denise Silva, Pereira, Paulo Roberto Gomes, Faria-Campos, Alessandra, Campos, Sérgio Vale Aguiar, Guimarães, José Domingos, Baracat-Pereira, Maria Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.theriogenology.2016.02.030
http://www.locus.ufv.br/handle/123456789/18954
Resumo: The Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm. Previously, the relationship of BSP1 with freezability events has not been completely understood. The objective of this work was to determine the differential abundance of the forms of the BSP1 protein in cryopreserved seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability using proteomics. A wide cohort of adult bulls with high genetic value from an artificial insemination center was used as donors of high quality, fresh semen. Nine bulls presenting different patterns of semen freezability were selected. Two-dimensional gel electrophoresis showed differential abundance in a group of seven protein spots in the frozen/thawed seminal plasma from the bulls, ranging from 15 to 17 kDa, with pI values from 4.6 to 5.8. Four of these spots were confirmed to be BSP1 using mass spectrometry, proteomics, biochemical, and computational analysis (Tukey's test at P < 0.05). The protein spot weighing 15.52 ± 0.53 kDa with a pI value of 5.78 ± 0.12 is highlighted by its high abundance in bulls with low semen freezability and its absence in bulls presenting high semen freezability. This is the first report showing that more than two forms of BSP1 are found in the seminal plasma of Nelore adult bulls and not all animals have a similar abundance of each BSP1 form. Different BSP1 forms may be involved in different events of fertilization and the cryopreservation process.
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spelling Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezabilityNelore bullsBSP1Semen cryopreservationFreezabilityProteomics Computational toolsThe Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm. Previously, the relationship of BSP1 with freezability events has not been completely understood. The objective of this work was to determine the differential abundance of the forms of the BSP1 protein in cryopreserved seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability using proteomics. A wide cohort of adult bulls with high genetic value from an artificial insemination center was used as donors of high quality, fresh semen. Nine bulls presenting different patterns of semen freezability were selected. Two-dimensional gel electrophoresis showed differential abundance in a group of seven protein spots in the frozen/thawed seminal plasma from the bulls, ranging from 15 to 17 kDa, with pI values from 4.6 to 5.8. Four of these spots were confirmed to be BSP1 using mass spectrometry, proteomics, biochemical, and computational analysis (Tukey's test at P < 0.05). The protein spot weighing 15.52 ± 0.53 kDa with a pI value of 5.78 ± 0.12 is highlighted by its high abundance in bulls with low semen freezability and its absence in bulls presenting high semen freezability. This is the first report showing that more than two forms of BSP1 are found in the seminal plasma of Nelore adult bulls and not all animals have a similar abundance of each BSP1 form. Different BSP1 forms may be involved in different events of fertilization and the cryopreservation process.Theriogenology2018-04-20T14:49:06Z2018-04-20T14:49:06Z2016-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf0093-691Xhttps://doi.org/10.1016/j.theriogenology.2016.02.030http://www.locus.ufv.br/handle/123456789/18954engVolume 86, Issue 3, Pages 766–777, August 2016Elsevier Inc.info:eu-repo/semantics/openAccessMagalhães Jr, Marcos JorgeMartins, Leonardo FrancoSenra, Renato LimaSantos, Thaís Ferreira doOkano, Denise SilvaPereira, Paulo Roberto GomesFaria-Campos, AlessandraCampos, Sérgio Vale AguiarGuimarães, José DomingosBaracat-Pereira, Maria Cristinareponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:12:16Zoai:locus.ufv.br:123456789/18954Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:12:16LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
title Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
spellingShingle Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
Magalhães Jr, Marcos Jorge
Nelore bulls
BSP1
Semen cryopreservation
Freezability
Proteomics Computational tools
title_short Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
title_full Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
title_fullStr Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
title_full_unstemmed Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
title_sort Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
author Magalhães Jr, Marcos Jorge
author_facet Magalhães Jr, Marcos Jorge
Martins, Leonardo Franco
Senra, Renato Lima
Santos, Thaís Ferreira do
Okano, Denise Silva
Pereira, Paulo Roberto Gomes
Faria-Campos, Alessandra
Campos, Sérgio Vale Aguiar
Guimarães, José Domingos
Baracat-Pereira, Maria Cristina
author_role author
author2 Martins, Leonardo Franco
Senra, Renato Lima
Santos, Thaís Ferreira do
Okano, Denise Silva
Pereira, Paulo Roberto Gomes
Faria-Campos, Alessandra
Campos, Sérgio Vale Aguiar
Guimarães, José Domingos
Baracat-Pereira, Maria Cristina
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Magalhães Jr, Marcos Jorge
Martins, Leonardo Franco
Senra, Renato Lima
Santos, Thaís Ferreira do
Okano, Denise Silva
Pereira, Paulo Roberto Gomes
Faria-Campos, Alessandra
Campos, Sérgio Vale Aguiar
Guimarães, José Domingos
Baracat-Pereira, Maria Cristina
dc.subject.por.fl_str_mv Nelore bulls
BSP1
Semen cryopreservation
Freezability
Proteomics Computational tools
topic Nelore bulls
BSP1
Semen cryopreservation
Freezability
Proteomics Computational tools
description The Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm. Previously, the relationship of BSP1 with freezability events has not been completely understood. The objective of this work was to determine the differential abundance of the forms of the BSP1 protein in cryopreserved seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability using proteomics. A wide cohort of adult bulls with high genetic value from an artificial insemination center was used as donors of high quality, fresh semen. Nine bulls presenting different patterns of semen freezability were selected. Two-dimensional gel electrophoresis showed differential abundance in a group of seven protein spots in the frozen/thawed seminal plasma from the bulls, ranging from 15 to 17 kDa, with pI values from 4.6 to 5.8. Four of these spots were confirmed to be BSP1 using mass spectrometry, proteomics, biochemical, and computational analysis (Tukey's test at P < 0.05). The protein spot weighing 15.52 ± 0.53 kDa with a pI value of 5.78 ± 0.12 is highlighted by its high abundance in bulls with low semen freezability and its absence in bulls presenting high semen freezability. This is the first report showing that more than two forms of BSP1 are found in the seminal plasma of Nelore adult bulls and not all animals have a similar abundance of each BSP1 form. Different BSP1 forms may be involved in different events of fertilization and the cryopreservation process.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-27
2018-04-20T14:49:06Z
2018-04-20T14:49:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 0093-691X
https://doi.org/10.1016/j.theriogenology.2016.02.030
http://www.locus.ufv.br/handle/123456789/18954
identifier_str_mv 0093-691X
url https://doi.org/10.1016/j.theriogenology.2016.02.030
http://www.locus.ufv.br/handle/123456789/18954
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Volume 86, Issue 3, Pages 766–777, August 2016
dc.rights.driver.fl_str_mv Elsevier Inc.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Elsevier Inc.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Theriogenology
publisher.none.fl_str_mv Theriogenology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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