Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.theriogenology.2016.02.030 http://www.locus.ufv.br/handle/123456789/18954 |
Resumo: | The Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm. Previously, the relationship of BSP1 with freezability events has not been completely understood. The objective of this work was to determine the differential abundance of the forms of the BSP1 protein in cryopreserved seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability using proteomics. A wide cohort of adult bulls with high genetic value from an artificial insemination center was used as donors of high quality, fresh semen. Nine bulls presenting different patterns of semen freezability were selected. Two-dimensional gel electrophoresis showed differential abundance in a group of seven protein spots in the frozen/thawed seminal plasma from the bulls, ranging from 15 to 17 kDa, with pI values from 4.6 to 5.8. Four of these spots were confirmed to be BSP1 using mass spectrometry, proteomics, biochemical, and computational analysis (Tukey's test at P < 0.05). The protein spot weighing 15.52 ± 0.53 kDa with a pI value of 5.78 ± 0.12 is highlighted by its high abundance in bulls with low semen freezability and its absence in bulls presenting high semen freezability. This is the first report showing that more than two forms of BSP1 are found in the seminal plasma of Nelore adult bulls and not all animals have a similar abundance of each BSP1 form. Different BSP1 forms may be involved in different events of fertilization and the cryopreservation process. |
id |
UFV_bf94ce2be0b23dcde323be8652197a28 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/18954 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezabilityNelore bullsBSP1Semen cryopreservationFreezabilityProteomics Computational toolsThe Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm. Previously, the relationship of BSP1 with freezability events has not been completely understood. The objective of this work was to determine the differential abundance of the forms of the BSP1 protein in cryopreserved seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability using proteomics. A wide cohort of adult bulls with high genetic value from an artificial insemination center was used as donors of high quality, fresh semen. Nine bulls presenting different patterns of semen freezability were selected. Two-dimensional gel electrophoresis showed differential abundance in a group of seven protein spots in the frozen/thawed seminal plasma from the bulls, ranging from 15 to 17 kDa, with pI values from 4.6 to 5.8. Four of these spots were confirmed to be BSP1 using mass spectrometry, proteomics, biochemical, and computational analysis (Tukey's test at P < 0.05). The protein spot weighing 15.52 ± 0.53 kDa with a pI value of 5.78 ± 0.12 is highlighted by its high abundance in bulls with low semen freezability and its absence in bulls presenting high semen freezability. This is the first report showing that more than two forms of BSP1 are found in the seminal plasma of Nelore adult bulls and not all animals have a similar abundance of each BSP1 form. Different BSP1 forms may be involved in different events of fertilization and the cryopreservation process.Theriogenology2018-04-20T14:49:06Z2018-04-20T14:49:06Z2016-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf0093-691Xhttps://doi.org/10.1016/j.theriogenology.2016.02.030http://www.locus.ufv.br/handle/123456789/18954engVolume 86, Issue 3, Pages 766–777, August 2016Elsevier Inc.info:eu-repo/semantics/openAccessMagalhães Jr, Marcos JorgeMartins, Leonardo FrancoSenra, Renato LimaSantos, Thaís Ferreira doOkano, Denise SilvaPereira, Paulo Roberto GomesFaria-Campos, AlessandraCampos, Sérgio Vale AguiarGuimarães, José DomingosBaracat-Pereira, Maria Cristinareponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:12:16Zoai:locus.ufv.br:123456789/18954Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:12:16LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability |
title |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability |
spellingShingle |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability Magalhães Jr, Marcos Jorge Nelore bulls BSP1 Semen cryopreservation Freezability Proteomics Computational tools |
title_short |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability |
title_full |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability |
title_fullStr |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability |
title_full_unstemmed |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability |
title_sort |
Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability |
author |
Magalhães Jr, Marcos Jorge |
author_facet |
Magalhães Jr, Marcos Jorge Martins, Leonardo Franco Senra, Renato Lima Santos, Thaís Ferreira do Okano, Denise Silva Pereira, Paulo Roberto Gomes Faria-Campos, Alessandra Campos, Sérgio Vale Aguiar Guimarães, José Domingos Baracat-Pereira, Maria Cristina |
author_role |
author |
author2 |
Martins, Leonardo Franco Senra, Renato Lima Santos, Thaís Ferreira do Okano, Denise Silva Pereira, Paulo Roberto Gomes Faria-Campos, Alessandra Campos, Sérgio Vale Aguiar Guimarães, José Domingos Baracat-Pereira, Maria Cristina |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Magalhães Jr, Marcos Jorge Martins, Leonardo Franco Senra, Renato Lima Santos, Thaís Ferreira do Okano, Denise Silva Pereira, Paulo Roberto Gomes Faria-Campos, Alessandra Campos, Sérgio Vale Aguiar Guimarães, José Domingos Baracat-Pereira, Maria Cristina |
dc.subject.por.fl_str_mv |
Nelore bulls BSP1 Semen cryopreservation Freezability Proteomics Computational tools |
topic |
Nelore bulls BSP1 Semen cryopreservation Freezability Proteomics Computational tools |
description |
The Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm. Previously, the relationship of BSP1 with freezability events has not been completely understood. The objective of this work was to determine the differential abundance of the forms of the BSP1 protein in cryopreserved seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability using proteomics. A wide cohort of adult bulls with high genetic value from an artificial insemination center was used as donors of high quality, fresh semen. Nine bulls presenting different patterns of semen freezability were selected. Two-dimensional gel electrophoresis showed differential abundance in a group of seven protein spots in the frozen/thawed seminal plasma from the bulls, ranging from 15 to 17 kDa, with pI values from 4.6 to 5.8. Four of these spots were confirmed to be BSP1 using mass spectrometry, proteomics, biochemical, and computational analysis (Tukey's test at P < 0.05). The protein spot weighing 15.52 ± 0.53 kDa with a pI value of 5.78 ± 0.12 is highlighted by its high abundance in bulls with low semen freezability and its absence in bulls presenting high semen freezability. This is the first report showing that more than two forms of BSP1 are found in the seminal plasma of Nelore adult bulls and not all animals have a similar abundance of each BSP1 form. Different BSP1 forms may be involved in different events of fertilization and the cryopreservation process. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-27 2018-04-20T14:49:06Z 2018-04-20T14:49:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
0093-691X https://doi.org/10.1016/j.theriogenology.2016.02.030 http://www.locus.ufv.br/handle/123456789/18954 |
identifier_str_mv |
0093-691X |
url |
https://doi.org/10.1016/j.theriogenology.2016.02.030 http://www.locus.ufv.br/handle/123456789/18954 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Volume 86, Issue 3, Pages 766–777, August 2016 |
dc.rights.driver.fl_str_mv |
Elsevier Inc. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Elsevier Inc. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Theriogenology |
publisher.none.fl_str_mv |
Theriogenology |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1817559908156964864 |