Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23923 |
Resumo: | Proteus spp. are opportunistic multidrug resistant enterobacteria associated with diverse clinical diseases in domestic animals. However, Proteus infections in domestic animals are often misdiagnosed or considered contaminants in microbiological cultures rather than a primary agent of disease. Descriptions of Proteus infections in domestic animals are typically restricted to case reports, retrospective studies, or surveillance of other microorganisms. The present study investigated multiple antibiotic resistance indices, minimum inhibitory concentrations (MICs), and ESBL production in 73 strains of Proteus mirabilis (n = 69) and Proteus vulgaris (n = 4) isolated from domestic animals with various clinical manifestations. In dogs, the pathogen was most commonly associated with cystitis (48.21), enteritis (21.42%), otitis (14.29%), and conjunctivitis (3.57%). In bovines, the microorganism was predominant in cases of enteritis (22.22%), abscess (11.11%), otitis (11.11%), omphalitis (11.11%), and peritonitis (11.11%), and in organ fragments (11.11%). In equines (50.0%) and cats (100.0%), diarrhea was the main clinical sign. In vitro standard disk diffusion assay showed that the most effective antimicrobials against the isolates were imipenem (98.63), norfloxacin (95.89), amikacin (95.89), levofloxacin (90.41), ceftriaxone (87.64), and florfenicol (87.67). In contrast, the isolates commonly showed resistance to novobiocin (95.89), azithromycin (57.53), and trimethropim/sulfamethoxazole (39.73). Among the 73 isolates, the efficacy of amoxicillin/clavulanic acid, gentamicin, ceftriaxone, and ciprofloxacin according to MICs was 87.67%, 86.30%, 84.93%, and 82.19%, respectively. The MIC50 values of amoxicillin/clavulanic acid, ceftriaxone, ciprofloxacin, and gentamicin were, respectively, 1.0, 0.004, 0.03, and 1.0 µg/mL. Thirty-three strains (45.21%) showed a antimicrobial multiple resistance index of ? 0.3. Multidrug resistance profiles of isolates were observed most frequently in dogs (n = 25; 75.76%), particularly in those with cystitis (n = 13; 52.0%), followed by bovines (n = 4; 12.12%), equines (n = 2; 6.06%), and cats (n = 2; 6.06%). Two (2.7%) strains, obtained from canine skin and feces, were diagnosed phenotypically as ESBL-producers. Here, we observed the diversity of Proteus infections in domestic animals. The presence of multidrug-resistant isolates and ESBL-producers reinforces the need for appropriate antimicrobial use and in vitro antimicrobial tests to support therapy. |
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Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infectionÍndice de resistência múltipla aos antimicrobianos, concentração inibitória mínima e ESBL fenotípica em linhagens de Proteus mirabilis e Proteus vulgaris isoladas de diferentes afecções em animais domésticosProteus spMultidrug resistantExtended-spectrum beta-lactamaseLivestockCompanion animals.Proteus spMultirresistência aos antimicrobianosBeta-lactamases de espectro estendidoAnimais de produção e de companhiaManifestações clínicas.Proteus spp. are opportunistic multidrug resistant enterobacteria associated with diverse clinical diseases in domestic animals. However, Proteus infections in domestic animals are often misdiagnosed or considered contaminants in microbiological cultures rather than a primary agent of disease. Descriptions of Proteus infections in domestic animals are typically restricted to case reports, retrospective studies, or surveillance of other microorganisms. The present study investigated multiple antibiotic resistance indices, minimum inhibitory concentrations (MICs), and ESBL production in 73 strains of Proteus mirabilis (n = 69) and Proteus vulgaris (n = 4) isolated from domestic animals with various clinical manifestations. In dogs, the pathogen was most commonly associated with cystitis (48.21), enteritis (21.42%), otitis (14.29%), and conjunctivitis (3.57%). In bovines, the microorganism was predominant in cases of enteritis (22.22%), abscess (11.11%), otitis (11.11%), omphalitis (11.11%), and peritonitis (11.11%), and in organ fragments (11.11%). In equines (50.0%) and cats (100.0%), diarrhea was the main clinical sign. In vitro standard disk diffusion assay showed that the most effective antimicrobials against the isolates were imipenem (98.63), norfloxacin (95.89), amikacin (95.89), levofloxacin (90.41), ceftriaxone (87.64), and florfenicol (87.67). In contrast, the isolates commonly showed resistance to novobiocin (95.89), azithromycin (57.53), and trimethropim/sulfamethoxazole (39.73). Among the 73 isolates, the efficacy of amoxicillin/clavulanic acid, gentamicin, ceftriaxone, and ciprofloxacin according to MICs was 87.67%, 86.30%, 84.93%, and 82.19%, respectively. The MIC50 values of amoxicillin/clavulanic acid, ceftriaxone, ciprofloxacin, and gentamicin were, respectively, 1.0, 0.004, 0.03, and 1.0 µg/mL. Thirty-three strains (45.21%) showed a antimicrobial multiple resistance index of ? 0.3. Multidrug resistance profiles of isolates were observed most frequently in dogs (n = 25; 75.76%), particularly in those with cystitis (n = 13; 52.0%), followed by bovines (n = 4; 12.12%), equines (n = 2; 6.06%), and cats (n = 2; 6.06%). Two (2.7%) strains, obtained from canine skin and feces, were diagnosed phenotypically as ESBL-producers. Here, we observed the diversity of Proteus infections in domestic animals. The presence of multidrug-resistant isolates and ESBL-producers reinforces the need for appropriate antimicrobial use and in vitro antimicrobial tests to support therapy.Proteus spp. são enterobactérias oportunistas, multirresistentes aos antimicrobianos, associadas a diversas infecções em animais domésticos. No entanto, as infecções por Proteus em animais de produção e de companhia são negligenciadas ou, por vezes, o patógeno é considerado “contaminante”, ainda que em infecções como agente primário. Os registros de infecções por Proteus sp. em animais domésticos estão restritos aos relatos de casos, estudos retrospectivos ou compondo estudos com outros micro-organismos. O presente estudo investigou o índice de resistência múltipla (IRMA) e a concentração inibitória mínima (CIM) de 73 Proteus mirabilis (n=69) e Proteus vulgaris (n=4) a diferentes antimicrobianos, bem com a produção fenotípica de ESBL, em isolados obtidos de várias manifestações clínicas em animais domésticos. Em cães, o micro-organismo foi identificado predominantemente em casos de cistite (48,21%), enterite (21,42%), otite (14,29%) e conjuntivite (3,57%). Nos bovinos, o agente foi isolado predominantemente de casos enterite (22,22%), abscesso (11,11%), otite (11,11%), onfalite (11,11%), peritonite (11,11%), metrite (11,11%) e em fragmento de órgão (11,11%). Nos equinos (50,0%) e felinos (100,0%) o micro-organismo foi isolado principalmente de enterite. A maior sensibilidade dos isolados no teste “in vitro” de difusão com discos foi observada para imipeném (98,63%), norfloxacino (95,89%), amicacina (95,89%), levofloxacino (90,41%), ceftriaxona (87,64%) e florfenicol (87,67%), enquanto a maior resistência das linhagens foi observada para novobiocina (95,89%), azitromicina (57,53%) e sulfametoxazole-trimetropim (39,73%). Dentre as 73 linhagens, a eficácia da amoxicilina/ácido clavulânico, gentamicina, ceftriaxona e ciprofloxacino utilizando o teste de CIM foi, respectivamente, 87,67%, 86,30%, 84,93% e 82,19%. A CIM50 para amoxicilina/ácido clavulânico, ceftriaxona, ciprofloxaicno e gentamicina foi, respectivamente, 1,0 µg/mL, 0,004 µg/mL, 0,03 µg/mL e 1,0 µg/mL. O índice de resistência múltipla aos antimicrobianos (IRMA ? 0,3) foi observado em 33 (45,21%) linhagens, variando entre 0,1 a 1. Entre os isolados com perfis de multirresistência, a maior ocorrência foi observada em cães (n=25; 75,76%), particularmente em animais com cistite (n=13; 52,0%), seguido pelos bovinos (n=4; 12,12%), equinos (n=2; 6,06%) e felinos (n=2; 6,06%). A presença fenotípica de ESBL foi identificada em dois (2,7%) isolados obtidos de dermatite e fezes de cães. Infere-se a diversidade de infecções causadas por Proteus em animais domésticos, a presença de isolados ESBL-positivos e multirresistentes aos antimicrobianos, reforçando a importância da instituição do tratamento do patógeno com respaldo em testes “in vitro” de sensibilidade microbiana e do uso racional de antimicrobianos no tratamento de infecções em animais domésticos.UEL2017-05-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2392310.5433/1679-0359.2017v38n2p775Semina: Ciências Agrárias; Vol. 38 No. 2 (2017); 775-790Semina: Ciências Agrárias; v. 38 n. 2 (2017); 775-7901679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23923/20874Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessZappa, VanessaBolaños, Carmen Alicia DazaPaula, Carolina Lechinski deCallefe, João Luis RevoltaAlves, Ana CarolinaMorais, Amanda Bonalume Cordeiro deGuerra, Simony TrevizanCabrini, Marina ChavesMelville, Priscilla AnneRibeiro, Márcio Garcia2022-11-16T16:36:57Zoai:ojs.pkp.sfu.ca:article/23923Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-16T16:36:57Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection Índice de resistência múltipla aos antimicrobianos, concentração inibitória mínima e ESBL fenotípica em linhagens de Proteus mirabilis e Proteus vulgaris isoladas de diferentes afecções em animais domésticos |
title |
Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection |
spellingShingle |
Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection Zappa, Vanessa Proteus sp Multidrug resistant Extended-spectrum beta-lactamase Livestock Companion animals. Proteus sp Multirresistência aos antimicrobianos Beta-lactamases de espectro estendido Animais de produção e de companhia Manifestações clínicas. |
title_short |
Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection |
title_full |
Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection |
title_fullStr |
Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection |
title_full_unstemmed |
Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection |
title_sort |
Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection |
author |
Zappa, Vanessa |
author_facet |
Zappa, Vanessa Bolaños, Carmen Alicia Daza Paula, Carolina Lechinski de Callefe, João Luis Revolta Alves, Ana Carolina Morais, Amanda Bonalume Cordeiro de Guerra, Simony Trevizan Cabrini, Marina Chaves Melville, Priscilla Anne Ribeiro, Márcio Garcia |
author_role |
author |
author2 |
Bolaños, Carmen Alicia Daza Paula, Carolina Lechinski de Callefe, João Luis Revolta Alves, Ana Carolina Morais, Amanda Bonalume Cordeiro de Guerra, Simony Trevizan Cabrini, Marina Chaves Melville, Priscilla Anne Ribeiro, Márcio Garcia |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Zappa, Vanessa Bolaños, Carmen Alicia Daza Paula, Carolina Lechinski de Callefe, João Luis Revolta Alves, Ana Carolina Morais, Amanda Bonalume Cordeiro de Guerra, Simony Trevizan Cabrini, Marina Chaves Melville, Priscilla Anne Ribeiro, Márcio Garcia |
dc.subject.por.fl_str_mv |
Proteus sp Multidrug resistant Extended-spectrum beta-lactamase Livestock Companion animals. Proteus sp Multirresistência aos antimicrobianos Beta-lactamases de espectro estendido Animais de produção e de companhia Manifestações clínicas. |
topic |
Proteus sp Multidrug resistant Extended-spectrum beta-lactamase Livestock Companion animals. Proteus sp Multirresistência aos antimicrobianos Beta-lactamases de espectro estendido Animais de produção e de companhia Manifestações clínicas. |
description |
Proteus spp. are opportunistic multidrug resistant enterobacteria associated with diverse clinical diseases in domestic animals. However, Proteus infections in domestic animals are often misdiagnosed or considered contaminants in microbiological cultures rather than a primary agent of disease. Descriptions of Proteus infections in domestic animals are typically restricted to case reports, retrospective studies, or surveillance of other microorganisms. The present study investigated multiple antibiotic resistance indices, minimum inhibitory concentrations (MICs), and ESBL production in 73 strains of Proteus mirabilis (n = 69) and Proteus vulgaris (n = 4) isolated from domestic animals with various clinical manifestations. In dogs, the pathogen was most commonly associated with cystitis (48.21), enteritis (21.42%), otitis (14.29%), and conjunctivitis (3.57%). In bovines, the microorganism was predominant in cases of enteritis (22.22%), abscess (11.11%), otitis (11.11%), omphalitis (11.11%), and peritonitis (11.11%), and in organ fragments (11.11%). In equines (50.0%) and cats (100.0%), diarrhea was the main clinical sign. In vitro standard disk diffusion assay showed that the most effective antimicrobials against the isolates were imipenem (98.63), norfloxacin (95.89), amikacin (95.89), levofloxacin (90.41), ceftriaxone (87.64), and florfenicol (87.67). In contrast, the isolates commonly showed resistance to novobiocin (95.89), azithromycin (57.53), and trimethropim/sulfamethoxazole (39.73). Among the 73 isolates, the efficacy of amoxicillin/clavulanic acid, gentamicin, ceftriaxone, and ciprofloxacin according to MICs was 87.67%, 86.30%, 84.93%, and 82.19%, respectively. The MIC50 values of amoxicillin/clavulanic acid, ceftriaxone, ciprofloxacin, and gentamicin were, respectively, 1.0, 0.004, 0.03, and 1.0 µg/mL. Thirty-three strains (45.21%) showed a antimicrobial multiple resistance index of ? 0.3. Multidrug resistance profiles of isolates were observed most frequently in dogs (n = 25; 75.76%), particularly in those with cystitis (n = 13; 52.0%), followed by bovines (n = 4; 12.12%), equines (n = 2; 6.06%), and cats (n = 2; 6.06%). Two (2.7%) strains, obtained from canine skin and feces, were diagnosed phenotypically as ESBL-producers. Here, we observed the diversity of Proteus infections in domestic animals. The presence of multidrug-resistant isolates and ESBL-producers reinforces the need for appropriate antimicrobial use and in vitro antimicrobial tests to support therapy. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23923 10.5433/1679-0359.2017v38n2p775 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23923 |
identifier_str_mv |
10.5433/1679-0359.2017v38n2p775 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23923/20874 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 38 No. 2 (2017); 775-790 Semina: Ciências Agrárias; v. 38 n. 2 (2017); 775-790 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306075235680256 |