Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.

Detalhes bibliográficos
Autor(a) principal: Ramos, Carlos Eduardo Crispim de Oliveira
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/1519
Resumo: Aiming to evaluate the milk contamination in the DPS for mycotoxins and chemical residues of organophosphates and carbamates it was made a study encompassing 95 SPL in three regions of Parana state. There where collected samples of milk, water and food and they were evaluated for chemical residues in all samples and aflatoxin only for food and milk. Mycotoxins in food (aflatoxin B1, B2, G1, G2, zearalenone and ochratoxin ) were detected by the method of thin layer chromatography - TLC and for the determination of aflatoxin M1 was used an immunoassay kit competitive ELISA Ridascreen®. The residues of organophosphates and carbamates was performed by colorimetric method qualitatively. There were evaluated the differences between regions, periods and the sources of mycotoxin contamination. Carbamates and organophosphates were screened for their presence in milk and the sources of food and water. Then it was estimated the contributions of each mycotoxin for milk contamination, as well as their respective contaminated food. Differences were found between periods (p < 0.05) for milk contamination with aflatoxin M1. For carbamates and organophosphates were found a different contamination sources (p < 0.01), and for the first pesticides used to parasitic herd control and for other pesticides used in agriculture. For food sources contamination resulting in the AFM1 contamination it was detected that AFB1 was the main source for the first. The AFG1 showed a strong correlation (p < 0.01) with AFB1 levels suggesting a causal relationship is a function of fungal strains producing both at the same time. It was also found the prevalence of aflatoxin contamination in 70% of contaminated samples and its predominant presence in relation to others in all kinds of foods analyzed. By identifying the checkpoints of contamination we can propose the inclusion of practical management methods to avoid this.
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spelling Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.Contamination by mycotoxins, organophosphates and carbamates : influences in milk quality.Gado leiteiroProduçãoMicotoxinas e pesticidasContaminaçãoAlimentaçãoLeiteBovinocultura leiteiraAlimentação do rebanhoBrasil.MycotoxinsDairy productionPesticidesHerd feedingMilkBrazil.Ciências AgráriasZootecniaAiming to evaluate the milk contamination in the DPS for mycotoxins and chemical residues of organophosphates and carbamates it was made a study encompassing 95 SPL in three regions of Parana state. There where collected samples of milk, water and food and they were evaluated for chemical residues in all samples and aflatoxin only for food and milk. Mycotoxins in food (aflatoxin B1, B2, G1, G2, zearalenone and ochratoxin ) were detected by the method of thin layer chromatography - TLC and for the determination of aflatoxin M1 was used an immunoassay kit competitive ELISA Ridascreen®. The residues of organophosphates and carbamates was performed by colorimetric method qualitatively. There were evaluated the differences between regions, periods and the sources of mycotoxin contamination. Carbamates and organophosphates were screened for their presence in milk and the sources of food and water. Then it was estimated the contributions of each mycotoxin for milk contamination, as well as their respective contaminated food. Differences were found between periods (p < 0.05) for milk contamination with aflatoxin M1. For carbamates and organophosphates were found a different contamination sources (p < 0.01), and for the first pesticides used to parasitic herd control and for other pesticides used in agriculture. For food sources contamination resulting in the AFM1 contamination it was detected that AFB1 was the main source for the first. The AFG1 showed a strong correlation (p < 0.01) with AFB1 levels suggesting a causal relationship is a function of fungal strains producing both at the same time. It was also found the prevalence of aflatoxin contamination in 70% of contaminated samples and its predominant presence in relation to others in all kinds of foods analyzed. By identifying the checkpoints of contamination we can propose the inclusion of practical management methods to avoid this.Para avaliar a contaminação do leite nos SPL tanto por micotoxinas quanto por resíduos químicos de organofosforados e carbamatos realizou-se um estudo englobando 95 SPL em três regiões no estado do Paraná. Colheram-se amostras de leite, água e alimentos, sendo avaliados os resíduos químicos para todas as amostras e de aflatoxinas apenas para os alimentos e o leite. As micotoxinas nos alimentos (Aflatoxinas B1, B2, G1, G2, Zearalenona e Ocratoxina) detectaram-se por meio do método de cromatografia de camada delgada - CCD e para a determinação da AFM1 utilizou-se um kit de imunoensaio ELISA competitivo, Ridascreen®. A determinação dos resíduos de carbamatos e organofosforados foi realizada por método colorimétrico qualitativo. Avaliaram-se as diferenças entre regiões, períodos e as fontes de contaminações por micotoxinas. Foram determinadas a presença ou ausência dos resíduos de carbamatos e organofosforados nas fontes de alimentação e água. Posteriormente, estimaram-se as contribuições de cada micotoxina para a contaminação do leite, bem como, a de seus respectivos alimentos contaminados. Observaram-se diferenças entre os períodos (p < 0,05) para a contaminação do leite com AFM1. Para os carbamatos e organofosforados detectou-se a distinção das fontes de contaminação (p < 0,01), sendo para os primeiros os pesticidas usados para o controle de parasitoses do rebanho, e para os outros, os agrotóxicos utilizados na agricultura. Para as fontes de contaminação alimentar originando a contaminação por AFM1 determinou-se que a AFB1 foi a principal fonte para a primeira. A AFG1 apresentou correlação (p < 0,01) com os níveis de AFB1 sugerindo uma relação causal com esta em função das cepas fúngicas produzindo ambas ao mesmo tempo. Constatou-se ainda a prevalência da contaminação por aflatoxinas em 70% das amostras contaminadas. Identificados os pontos de contaminação se pode propor intervenções no sentido de evitar estas ocorrências na cadeia de produção leiteira.82 f.,[8]Universidade Estadual de MaringáBrasilDepartamento de ZootecniaPrograma de Pós-Graduação em ZootecniaUEMMaringáCentro de Ciências AgráriasJulio Cesar DamascenoEliane Dalva Godoy Danesi - DTC/UEMMagali Soares dos Santos Pozza - CCA/Universidade Estadual do Oeste do ParanáCarlos Antonio Lopes de Oliveira - DZO/UEMFerenc Istvan Bankuti - DZO/UEMRamos, Carlos Eduardo Crispim de Oliveira2018-04-06T16:48:11Z2018-04-06T16:48:11Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://repositorio.uem.br:8080/jspui/handle/1/1519porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)instname:Universidade Estadual de Maringá (UEM)instacron:UEM2018-10-16T17:22:41Zoai:localhost:1/1519Repositório InstitucionalPUBhttp://repositorio.uem.br:8080/oai/requestopendoar:2024-04-23T14:54:28.511046Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.
Contamination by mycotoxins, organophosphates and carbamates : influences in milk quality.
title Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.
spellingShingle Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.
Ramos, Carlos Eduardo Crispim de Oliveira
Gado leiteiro
Produção
Micotoxinas e pesticidas
Contaminação
Alimentação
Leite
Bovinocultura leiteira
Alimentação do rebanho
Brasil.
Mycotoxins
Dairy production
Pesticides
Herd feeding
Milk
Brazil.
Ciências Agrárias
Zootecnia
title_short Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.
title_full Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.
title_fullStr Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.
title_full_unstemmed Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.
title_sort Contaminação por micotoxinas, resíduos de organofosforados e carbamatos : influências na qualidade do leite.
author Ramos, Carlos Eduardo Crispim de Oliveira
author_facet Ramos, Carlos Eduardo Crispim de Oliveira
author_role author
dc.contributor.none.fl_str_mv Julio Cesar Damasceno
Eliane Dalva Godoy Danesi - DTC/UEM
Magali Soares dos Santos Pozza - CCA/Universidade Estadual do Oeste do Paraná
Carlos Antonio Lopes de Oliveira - DZO/UEM
Ferenc Istvan Bankuti - DZO/UEM
dc.contributor.author.fl_str_mv Ramos, Carlos Eduardo Crispim de Oliveira
dc.subject.por.fl_str_mv Gado leiteiro
Produção
Micotoxinas e pesticidas
Contaminação
Alimentação
Leite
Bovinocultura leiteira
Alimentação do rebanho
Brasil.
Mycotoxins
Dairy production
Pesticides
Herd feeding
Milk
Brazil.
Ciências Agrárias
Zootecnia
topic Gado leiteiro
Produção
Micotoxinas e pesticidas
Contaminação
Alimentação
Leite
Bovinocultura leiteira
Alimentação do rebanho
Brasil.
Mycotoxins
Dairy production
Pesticides
Herd feeding
Milk
Brazil.
Ciências Agrárias
Zootecnia
description Aiming to evaluate the milk contamination in the DPS for mycotoxins and chemical residues of organophosphates and carbamates it was made a study encompassing 95 SPL in three regions of Parana state. There where collected samples of milk, water and food and they were evaluated for chemical residues in all samples and aflatoxin only for food and milk. Mycotoxins in food (aflatoxin B1, B2, G1, G2, zearalenone and ochratoxin ) were detected by the method of thin layer chromatography - TLC and for the determination of aflatoxin M1 was used an immunoassay kit competitive ELISA Ridascreen®. The residues of organophosphates and carbamates was performed by colorimetric method qualitatively. There were evaluated the differences between regions, periods and the sources of mycotoxin contamination. Carbamates and organophosphates were screened for their presence in milk and the sources of food and water. Then it was estimated the contributions of each mycotoxin for milk contamination, as well as their respective contaminated food. Differences were found between periods (p < 0.05) for milk contamination with aflatoxin M1. For carbamates and organophosphates were found a different contamination sources (p < 0.01), and for the first pesticides used to parasitic herd control and for other pesticides used in agriculture. For food sources contamination resulting in the AFM1 contamination it was detected that AFB1 was the main source for the first. The AFG1 showed a strong correlation (p < 0.01) with AFB1 levels suggesting a causal relationship is a function of fungal strains producing both at the same time. It was also found the prevalence of aflatoxin contamination in 70% of contaminated samples and its predominant presence in relation to others in all kinds of foods analyzed. By identifying the checkpoints of contamination we can propose the inclusion of practical management methods to avoid this.
publishDate 2011
dc.date.none.fl_str_mv 2011
2018-04-06T16:48:11Z
2018-04-06T16:48:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.uem.br:8080/jspui/handle/1/1519
url http://repositorio.uem.br:8080/jspui/handle/1/1519
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
Brasil
Departamento de Zootecnia
Programa de Pós-Graduação em Zootecnia
UEM
Maringá
Centro de Ciências Agrárias
publisher.none.fl_str_mv Universidade Estadual de Maringá
Brasil
Departamento de Zootecnia
Programa de Pós-Graduação em Zootecnia
UEM
Maringá
Centro de Ciências Agrárias
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
collection Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
repository.name.fl_str_mv Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv
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