Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino

Detalhes bibliográficos
Autor(a) principal: Silva, Larissa Albunio
Data de Publicação: 2014
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/3669
Resumo: The production of CGTase was performed by cells of Bacillus firmus strain 37 free in the reaction medium in a batch bioreactor and also by cells immobilized activated bovine bone char, an innovative support for this purpose, which was used in a jacketed fluidized bed column with fluid recirculation and temperature control. An experimental design was developed with three quantities of bovine bone char (3.5, 7 and 14 g ) and three aeration rates (0.5, 1 and 2 v/v/m). The use of bone char as support, aeration of the fermentation medium and the fluidized bed colunm contributed to a higher production yield of the enzyme, because the enzyme activities found were the highest compared wthi free cell. At the end of 120 h of production assays using 7 and 14 g of bone char and aeration rate equal to 2 v/v/m the CGTase activity obtained was equal to 2.5 U/mL. Furthermore, it was found that the amount of the intermediate bone char (7 g) using the maximum rate of aeration (2 v/v/m), the test was that the most significant values obtained CGTase activity, i.e., enzyme activity of 1.75 U/mL at 96 h. In contrast, for the assays using the microorganism free in the reaction medium or without aeration, the activity was found to be 0.7 U/mL after 96 h of production. Therefore, it was concluded that both aeration, the immobilization of the microorganism on bone char and the fluidized bed bioreactor were instrumental in increasing the production of CGTase. A statistical model of second order revealed that the independent variables (aeration rate and amount of bone char), the values of the enzymatic activity for the cultivation of 120 h. However , an assay using 28 g of bone char and 4 v/v/m, did not increase the activity, which remained at 2.5 U/mL, but showed a reduction in the enzyme production time that was halved to 60 h. This study has demonstrated the possibility of producing CGTase using Bacillus firmus strain 37 immobilized activated bovine bone char and showed relatively high values of CGTase enzyme activities, compared to other support matrices.
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spelling Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovinoCGTase imobilizadaBacillus firmusCiclodextrinaCarvão de osso bovinoBrasil.ProductionCGTaseBacillus firmusImmobilizationBovine bone charBrazil.EngenhariasEngenharia QuímicaThe production of CGTase was performed by cells of Bacillus firmus strain 37 free in the reaction medium in a batch bioreactor and also by cells immobilized activated bovine bone char, an innovative support for this purpose, which was used in a jacketed fluidized bed column with fluid recirculation and temperature control. An experimental design was developed with three quantities of bovine bone char (3.5, 7 and 14 g ) and three aeration rates (0.5, 1 and 2 v/v/m). The use of bone char as support, aeration of the fermentation medium and the fluidized bed colunm contributed to a higher production yield of the enzyme, because the enzyme activities found were the highest compared wthi free cell. At the end of 120 h of production assays using 7 and 14 g of bone char and aeration rate equal to 2 v/v/m the CGTase activity obtained was equal to 2.5 U/mL. Furthermore, it was found that the amount of the intermediate bone char (7 g) using the maximum rate of aeration (2 v/v/m), the test was that the most significant values obtained CGTase activity, i.e., enzyme activity of 1.75 U/mL at 96 h. In contrast, for the assays using the microorganism free in the reaction medium or without aeration, the activity was found to be 0.7 U/mL after 96 h of production. Therefore, it was concluded that both aeration, the immobilization of the microorganism on bone char and the fluidized bed bioreactor were instrumental in increasing the production of CGTase. A statistical model of second order revealed that the independent variables (aeration rate and amount of bone char), the values of the enzymatic activity for the cultivation of 120 h. However , an assay using 28 g of bone char and 4 v/v/m, did not increase the activity, which remained at 2.5 U/mL, but showed a reduction in the enzyme production time that was halved to 60 h. This study has demonstrated the possibility of producing CGTase using Bacillus firmus strain 37 immobilized activated bovine bone char and showed relatively high values of CGTase enzyme activities, compared to other support matrices.A produção de CGTase foi realizada por células de Bacillus firmus cepa 37, livres no meio de reação num biorreator batelada e também por células imobilizadas em carvão ativado de osso bovino, um suporte inovador para esta aplicação, o qual foi utilizado numa coluna de leito fluidizado jaquetada, com recirculação de fluido para controle da temperatura. Um planejamento experimental foi desenvolvido com três quantidades de carvão de osso bovino (3,5; 7 e 14 g) e três taxas de aeração (0,5; 1 e 2 v/v/m). O uso de carvão ativado de osso como suporte, a aeração do meio de fermentação e a coluna de leito fluidizado contribuíram para uma maior produção da enzima, pois as atividades enzimáticas encontradas foram as maiores em comparação com o bacilos livre. Ao fim de 120 h de ensaios de produção a partir de 7 e 14 g de carvão de osso e taxa de aeração de 2 v/v/m a atividade de CGTase obtida foi de 2,5 U/mL. Além disso, verificou-se no que a quantidade intermediária de carvão de osso (7 g) utilizando a máxima taxa de aeração (2 v/v/m), foi o ensaio que obteve valores de atividade de CGTase mais significativos, ou seja, atividade enzimática de 1,75 U/mL em 96 h. Em contraste, para os ensaios utilizando o micro-organismo livre no meio de reação e sem aeração, a atividade encontrada foi de 0,7 U/mL, após 96 h de produção. Portanto, concluiu-se que tanto a aeração, a imobilização do micro-organismo em carvão de osso e, o biorreator de leito fluidizado, foram fundamentais para aumentar a produção de CGTase. Um modelo estatístico de segunda ordem indicou que se as variáveis independentes (quantidade de carvão de osso e a taxa de aeração) fossem aumentadas, o valor da atividade enzimática também seria maior, para um cultivo de 120 horas. No entanto, um ensaio utilizando 28 g de carvão de osso e 4 v/v/m, não conduziu ao aumento da atividade enzimática, que se manteve em 2,5 U/mL, mas mostrou uma diminuição no tempo de produção da enzima que foi reduzido a 60 h. Este estudo demonstrou a possibilidade de produção de CGTase com o Bacillus firmus cepa 37 imobilizado em carvão ativado de osso bovino e mostrou valores elevados de atividades enzimáticas de CGTase, em comparação com outras matrizes de suporte citadas na literatura.1 CD-ROM (128 f.)Universidade Estadual de MaringáBrasilDepartamento de Engenharia QuímicaPrograma de Pós-Graduação em Engenharia QuímicaUEMMaringá, PRCentro de TecnologiaFlávio Faria de MoraesGisella Maria Zanin - UEMJosé Eduardo Olivo - UEMPaulo Waldir Tardioli - UFScarMarcos de Souza - UEMSilva, Larissa Albunio2018-04-17T17:39:55Z2018-04-17T17:39:55Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://repositorio.uem.br:8080/jspui/handle/1/3669porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)instname:Universidade Estadual de Maringá (UEM)instacron:UEM2018-10-15T18:13:48Zoai:localhost:1/3669Repositório InstitucionalPUBhttp://repositorio.uem.br:8080/oai/requestopendoar:2024-04-23T14:56:49.088162Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
title Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
spellingShingle Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
Silva, Larissa Albunio
CGTase imobilizada
Bacillus firmus
Ciclodextrina
Carvão de osso bovino
Brasil.
Production
CGTase
Bacillus firmus
Immobilization
Bovine bone char
Brazil.
Engenharias
Engenharia Química
title_short Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
title_full Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
title_fullStr Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
title_full_unstemmed Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
title_sort Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
author Silva, Larissa Albunio
author_facet Silva, Larissa Albunio
author_role author
dc.contributor.none.fl_str_mv Flávio Faria de Moraes
Gisella Maria Zanin - UEM
José Eduardo Olivo - UEM
Paulo Waldir Tardioli - UFScar
Marcos de Souza - UEM
dc.contributor.author.fl_str_mv Silva, Larissa Albunio
dc.subject.por.fl_str_mv CGTase imobilizada
Bacillus firmus
Ciclodextrina
Carvão de osso bovino
Brasil.
Production
CGTase
Bacillus firmus
Immobilization
Bovine bone char
Brazil.
Engenharias
Engenharia Química
topic CGTase imobilizada
Bacillus firmus
Ciclodextrina
Carvão de osso bovino
Brasil.
Production
CGTase
Bacillus firmus
Immobilization
Bovine bone char
Brazil.
Engenharias
Engenharia Química
description The production of CGTase was performed by cells of Bacillus firmus strain 37 free in the reaction medium in a batch bioreactor and also by cells immobilized activated bovine bone char, an innovative support for this purpose, which was used in a jacketed fluidized bed column with fluid recirculation and temperature control. An experimental design was developed with three quantities of bovine bone char (3.5, 7 and 14 g ) and three aeration rates (0.5, 1 and 2 v/v/m). The use of bone char as support, aeration of the fermentation medium and the fluidized bed colunm contributed to a higher production yield of the enzyme, because the enzyme activities found were the highest compared wthi free cell. At the end of 120 h of production assays using 7 and 14 g of bone char and aeration rate equal to 2 v/v/m the CGTase activity obtained was equal to 2.5 U/mL. Furthermore, it was found that the amount of the intermediate bone char (7 g) using the maximum rate of aeration (2 v/v/m), the test was that the most significant values obtained CGTase activity, i.e., enzyme activity of 1.75 U/mL at 96 h. In contrast, for the assays using the microorganism free in the reaction medium or without aeration, the activity was found to be 0.7 U/mL after 96 h of production. Therefore, it was concluded that both aeration, the immobilization of the microorganism on bone char and the fluidized bed bioreactor were instrumental in increasing the production of CGTase. A statistical model of second order revealed that the independent variables (aeration rate and amount of bone char), the values of the enzymatic activity for the cultivation of 120 h. However , an assay using 28 g of bone char and 4 v/v/m, did not increase the activity, which remained at 2.5 U/mL, but showed a reduction in the enzyme production time that was halved to 60 h. This study has demonstrated the possibility of producing CGTase using Bacillus firmus strain 37 immobilized activated bovine bone char and showed relatively high values of CGTase enzyme activities, compared to other support matrices.
publishDate 2014
dc.date.none.fl_str_mv 2014
2018-04-17T17:39:55Z
2018-04-17T17:39:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.uem.br:8080/jspui/handle/1/3669
url http://repositorio.uem.br:8080/jspui/handle/1/3669
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
Brasil
Departamento de Engenharia Química
Programa de Pós-Graduação em Engenharia Química
UEM
Maringá, PR
Centro de Tecnologia
publisher.none.fl_str_mv Universidade Estadual de Maringá
Brasil
Departamento de Engenharia Química
Programa de Pós-Graduação em Engenharia Química
UEM
Maringá, PR
Centro de Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
collection Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
repository.name.fl_str_mv Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv
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