Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
Texto Completo: | http://repositorio.uem.br:8080/jspui/handle/1/3669 |
Resumo: | The production of CGTase was performed by cells of Bacillus firmus strain 37 free in the reaction medium in a batch bioreactor and also by cells immobilized activated bovine bone char, an innovative support for this purpose, which was used in a jacketed fluidized bed column with fluid recirculation and temperature control. An experimental design was developed with three quantities of bovine bone char (3.5, 7 and 14 g ) and three aeration rates (0.5, 1 and 2 v/v/m). The use of bone char as support, aeration of the fermentation medium and the fluidized bed colunm contributed to a higher production yield of the enzyme, because the enzyme activities found were the highest compared wthi free cell. At the end of 120 h of production assays using 7 and 14 g of bone char and aeration rate equal to 2 v/v/m the CGTase activity obtained was equal to 2.5 U/mL. Furthermore, it was found that the amount of the intermediate bone char (7 g) using the maximum rate of aeration (2 v/v/m), the test was that the most significant values obtained CGTase activity, i.e., enzyme activity of 1.75 U/mL at 96 h. In contrast, for the assays using the microorganism free in the reaction medium or without aeration, the activity was found to be 0.7 U/mL after 96 h of production. Therefore, it was concluded that both aeration, the immobilization of the microorganism on bone char and the fluidized bed bioreactor were instrumental in increasing the production of CGTase. A statistical model of second order revealed that the independent variables (aeration rate and amount of bone char), the values of the enzymatic activity for the cultivation of 120 h. However , an assay using 28 g of bone char and 4 v/v/m, did not increase the activity, which remained at 2.5 U/mL, but showed a reduction in the enzyme production time that was halved to 60 h. This study has demonstrated the possibility of producing CGTase using Bacillus firmus strain 37 immobilized activated bovine bone char and showed relatively high values of CGTase enzyme activities, compared to other support matrices. |
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Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovinoCGTase imobilizadaBacillus firmusCiclodextrinaCarvão de osso bovinoBrasil.ProductionCGTaseBacillus firmusImmobilizationBovine bone charBrazil.EngenhariasEngenharia QuímicaThe production of CGTase was performed by cells of Bacillus firmus strain 37 free in the reaction medium in a batch bioreactor and also by cells immobilized activated bovine bone char, an innovative support for this purpose, which was used in a jacketed fluidized bed column with fluid recirculation and temperature control. An experimental design was developed with three quantities of bovine bone char (3.5, 7 and 14 g ) and three aeration rates (0.5, 1 and 2 v/v/m). The use of bone char as support, aeration of the fermentation medium and the fluidized bed colunm contributed to a higher production yield of the enzyme, because the enzyme activities found were the highest compared wthi free cell. At the end of 120 h of production assays using 7 and 14 g of bone char and aeration rate equal to 2 v/v/m the CGTase activity obtained was equal to 2.5 U/mL. Furthermore, it was found that the amount of the intermediate bone char (7 g) using the maximum rate of aeration (2 v/v/m), the test was that the most significant values obtained CGTase activity, i.e., enzyme activity of 1.75 U/mL at 96 h. In contrast, for the assays using the microorganism free in the reaction medium or without aeration, the activity was found to be 0.7 U/mL after 96 h of production. Therefore, it was concluded that both aeration, the immobilization of the microorganism on bone char and the fluidized bed bioreactor were instrumental in increasing the production of CGTase. A statistical model of second order revealed that the independent variables (aeration rate and amount of bone char), the values of the enzymatic activity for the cultivation of 120 h. However , an assay using 28 g of bone char and 4 v/v/m, did not increase the activity, which remained at 2.5 U/mL, but showed a reduction in the enzyme production time that was halved to 60 h. This study has demonstrated the possibility of producing CGTase using Bacillus firmus strain 37 immobilized activated bovine bone char and showed relatively high values of CGTase enzyme activities, compared to other support matrices.A produção de CGTase foi realizada por células de Bacillus firmus cepa 37, livres no meio de reação num biorreator batelada e também por células imobilizadas em carvão ativado de osso bovino, um suporte inovador para esta aplicação, o qual foi utilizado numa coluna de leito fluidizado jaquetada, com recirculação de fluido para controle da temperatura. Um planejamento experimental foi desenvolvido com três quantidades de carvão de osso bovino (3,5; 7 e 14 g) e três taxas de aeração (0,5; 1 e 2 v/v/m). O uso de carvão ativado de osso como suporte, a aeração do meio de fermentação e a coluna de leito fluidizado contribuíram para uma maior produção da enzima, pois as atividades enzimáticas encontradas foram as maiores em comparação com o bacilos livre. Ao fim de 120 h de ensaios de produção a partir de 7 e 14 g de carvão de osso e taxa de aeração de 2 v/v/m a atividade de CGTase obtida foi de 2,5 U/mL. Além disso, verificou-se no que a quantidade intermediária de carvão de osso (7 g) utilizando a máxima taxa de aeração (2 v/v/m), foi o ensaio que obteve valores de atividade de CGTase mais significativos, ou seja, atividade enzimática de 1,75 U/mL em 96 h. Em contraste, para os ensaios utilizando o micro-organismo livre no meio de reação e sem aeração, a atividade encontrada foi de 0,7 U/mL, após 96 h de produção. Portanto, concluiu-se que tanto a aeração, a imobilização do micro-organismo em carvão de osso e, o biorreator de leito fluidizado, foram fundamentais para aumentar a produção de CGTase. Um modelo estatístico de segunda ordem indicou que se as variáveis independentes (quantidade de carvão de osso e a taxa de aeração) fossem aumentadas, o valor da atividade enzimática também seria maior, para um cultivo de 120 horas. No entanto, um ensaio utilizando 28 g de carvão de osso e 4 v/v/m, não conduziu ao aumento da atividade enzimática, que se manteve em 2,5 U/mL, mas mostrou uma diminuição no tempo de produção da enzima que foi reduzido a 60 h. Este estudo demonstrou a possibilidade de produção de CGTase com o Bacillus firmus cepa 37 imobilizado em carvão ativado de osso bovino e mostrou valores elevados de atividades enzimáticas de CGTase, em comparação com outras matrizes de suporte citadas na literatura.1 CD-ROM (128 f.)Universidade Estadual de MaringáBrasilDepartamento de Engenharia QuímicaPrograma de Pós-Graduação em Engenharia QuímicaUEMMaringá, PRCentro de TecnologiaFlávio Faria de MoraesGisella Maria Zanin - UEMJosé Eduardo Olivo - UEMPaulo Waldir Tardioli - UFScarMarcos de Souza - UEMSilva, Larissa Albunio2018-04-17T17:39:55Z2018-04-17T17:39:55Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://repositorio.uem.br:8080/jspui/handle/1/3669porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)instname:Universidade Estadual de Maringá (UEM)instacron:UEM2018-10-15T18:13:48Zoai:localhost:1/3669Repositório InstitucionalPUBhttp://repositorio.uem.br:8080/oai/requestopendoar:2024-04-23T14:56:49.088162Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino |
title |
Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino |
spellingShingle |
Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino Silva, Larissa Albunio CGTase imobilizada Bacillus firmus Ciclodextrina Carvão de osso bovino Brasil. Production CGTase Bacillus firmus Immobilization Bovine bone char Brazil. Engenharias Engenharia Química |
title_short |
Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino |
title_full |
Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino |
title_fullStr |
Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino |
title_full_unstemmed |
Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino |
title_sort |
Produção de ciclomaltodextrina-glucanotrasferase com Bacillus firmus cepa 37 imobilizado em carvão de osso bovino |
author |
Silva, Larissa Albunio |
author_facet |
Silva, Larissa Albunio |
author_role |
author |
dc.contributor.none.fl_str_mv |
Flávio Faria de Moraes Gisella Maria Zanin - UEM José Eduardo Olivo - UEM Paulo Waldir Tardioli - UFScar Marcos de Souza - UEM |
dc.contributor.author.fl_str_mv |
Silva, Larissa Albunio |
dc.subject.por.fl_str_mv |
CGTase imobilizada Bacillus firmus Ciclodextrina Carvão de osso bovino Brasil. Production CGTase Bacillus firmus Immobilization Bovine bone char Brazil. Engenharias Engenharia Química |
topic |
CGTase imobilizada Bacillus firmus Ciclodextrina Carvão de osso bovino Brasil. Production CGTase Bacillus firmus Immobilization Bovine bone char Brazil. Engenharias Engenharia Química |
description |
The production of CGTase was performed by cells of Bacillus firmus strain 37 free in the reaction medium in a batch bioreactor and also by cells immobilized activated bovine bone char, an innovative support for this purpose, which was used in a jacketed fluidized bed column with fluid recirculation and temperature control. An experimental design was developed with three quantities of bovine bone char (3.5, 7 and 14 g ) and three aeration rates (0.5, 1 and 2 v/v/m). The use of bone char as support, aeration of the fermentation medium and the fluidized bed colunm contributed to a higher production yield of the enzyme, because the enzyme activities found were the highest compared wthi free cell. At the end of 120 h of production assays using 7 and 14 g of bone char and aeration rate equal to 2 v/v/m the CGTase activity obtained was equal to 2.5 U/mL. Furthermore, it was found that the amount of the intermediate bone char (7 g) using the maximum rate of aeration (2 v/v/m), the test was that the most significant values obtained CGTase activity, i.e., enzyme activity of 1.75 U/mL at 96 h. In contrast, for the assays using the microorganism free in the reaction medium or without aeration, the activity was found to be 0.7 U/mL after 96 h of production. Therefore, it was concluded that both aeration, the immobilization of the microorganism on bone char and the fluidized bed bioreactor were instrumental in increasing the production of CGTase. A statistical model of second order revealed that the independent variables (aeration rate and amount of bone char), the values of the enzymatic activity for the cultivation of 120 h. However , an assay using 28 g of bone char and 4 v/v/m, did not increase the activity, which remained at 2.5 U/mL, but showed a reduction in the enzyme production time that was halved to 60 h. This study has demonstrated the possibility of producing CGTase using Bacillus firmus strain 37 immobilized activated bovine bone char and showed relatively high values of CGTase enzyme activities, compared to other support matrices. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2018-04-17T17:39:55Z 2018-04-17T17:39:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.uem.br:8080/jspui/handle/1/3669 |
url |
http://repositorio.uem.br:8080/jspui/handle/1/3669 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá Brasil Departamento de Engenharia Química Programa de Pós-Graduação em Engenharia Química UEM Maringá, PR Centro de Tecnologia |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá Brasil Departamento de Engenharia Química Programa de Pós-Graduação em Engenharia Química UEM Maringá, PR Centro de Tecnologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
collection |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
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