Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
Texto Completo: | http://repositorio.uem.br:8080/jspui/handle/1/1901 |
Resumo: | Tuberculosis (TB) is an important public health problem worldwide and the leading cause of deaths by an infectious agent. The objective of this study was provide information about genetic diversity of Mycobacterium tuberculosis, isolated from patients with pulmonary TB attended at the Laboratório de Ensino e Pesquisa em Análises Clínicas (LEPAC), using Spoligotyping and MIRU-VNTR methodologies. The results were reported in the paper "Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil". From the Spoligotyping and MIRU-VNTR patterns of M. tuberculosis isolates dendrograms were constructed to evaluate the relationship of the isolates. The results show that TB in our region predominantly develops from endogenous reactivation of a latent infection. It was also carried out a comparison of discriminatory power between the methodologies used (Spoligotyping and MIRU-VNTR), obtaining better discriminatory capacity of the isolates combining the two methodologies. |
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Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of BrazilTuberculoseMycobacterium tuberculosisEpidemiologia molecularSpoligotypingMIRUBrasil.TuberculosisMycobacterium tuberculosisMolecular EpidemiologySpoligotypingMIRUBrazil.Ciências da SaúdeFarmáciaTuberculosis (TB) is an important public health problem worldwide and the leading cause of deaths by an infectious agent. The objective of this study was provide information about genetic diversity of Mycobacterium tuberculosis, isolated from patients with pulmonary TB attended at the Laboratório de Ensino e Pesquisa em Análises Clínicas (LEPAC), using Spoligotyping and MIRU-VNTR methodologies. The results were reported in the paper "Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil". From the Spoligotyping and MIRU-VNTR patterns of M. tuberculosis isolates dendrograms were constructed to evaluate the relationship of the isolates. The results show that TB in our region predominantly develops from endogenous reactivation of a latent infection. It was also carried out a comparison of discriminatory power between the methodologies used (Spoligotyping and MIRU-VNTR), obtaining better discriminatory capacity of the isolates combining the two methodologies.A tuberculose (TB) é um importante problema de saúde pública mundial, sendo a principal causa de mortes devido a um agente infeccioso. O objetivo desse trabalho foi fornecer informação sobre a diversidade genética de Mycobacterium tuberculosis, isolados de pacientes com TB pulmonar atendidos no Laboratório de Ensino e Pesquisa em Análises Clínicas (LEPAC), utilizando as metodologias Spoligotyping e MIRU-VNTR. Os resultados foram relatados no artigo "Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil". Com os perfis de Spoligotyping e MIRU-VNTR dos isolados de M. tuberculosis foram construídos dendrogramas para avaliar o grau de relação entre os isolados. A partir desta avaliação pode-se verificar que a TB em nossa região desenvolve-se predominantemente devido à reativação endógena de uma infecção latente. Foi realizada também uma comparação da capacidade discriminatória entre as metodologias utilizadas (Spoligotyping e MIRU-VNTR), obtendo-se melhor capacidade discriminatória dos isolados combinando as duas metodologias.43 fUniversidade Estadual de MaringáBrasilDepartamento de Análises ClínicasPrograma de Pós-Graduação em Biociências Aplicadas à FarmáciaUEMMaringá, PRCentro de Ciências da SaúdeRosilene Fressatti CardosoThais Gomes Verzignassi Silveira - UEMEliana Valéria Patussi - UEMJosé Rodrigo Cláudio Pandolfi - Universidade Camilo Castelo BrancoDaisy Nakamura Sato - Instituto Adolfo LutzNoguti, Erika Noda2018-04-06T19:45:55Z2018-04-06T19:45:55Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://repositorio.uem.br:8080/jspui/handle/1/1901porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)instname:Universidade Estadual de Maringá (UEM)instacron:UEM2018-10-08T17:20:27Zoai:localhost:1/1901Repositório InstitucionalPUBhttp://repositorio.uem.br:8080/oai/requestopendoar:2024-04-23T14:54:54.256309Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil |
title |
Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil |
spellingShingle |
Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil Noguti, Erika Noda Tuberculose Mycobacterium tuberculosis Epidemiologia molecular Spoligotyping MIRU Brasil. Tuberculosis Mycobacterium tuberculosis Molecular Epidemiology Spoligotyping MIRU Brazil. Ciências da Saúde Farmácia |
title_short |
Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil |
title_full |
Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil |
title_fullStr |
Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil |
title_full_unstemmed |
Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil |
title_sort |
Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil |
author |
Noguti, Erika Noda |
author_facet |
Noguti, Erika Noda |
author_role |
author |
dc.contributor.none.fl_str_mv |
Rosilene Fressatti Cardoso Thais Gomes Verzignassi Silveira - UEM Eliana Valéria Patussi - UEM José Rodrigo Cláudio Pandolfi - Universidade Camilo Castelo Branco Daisy Nakamura Sato - Instituto Adolfo Lutz |
dc.contributor.author.fl_str_mv |
Noguti, Erika Noda |
dc.subject.por.fl_str_mv |
Tuberculose Mycobacterium tuberculosis Epidemiologia molecular Spoligotyping MIRU Brasil. Tuberculosis Mycobacterium tuberculosis Molecular Epidemiology Spoligotyping MIRU Brazil. Ciências da Saúde Farmácia |
topic |
Tuberculose Mycobacterium tuberculosis Epidemiologia molecular Spoligotyping MIRU Brasil. Tuberculosis Mycobacterium tuberculosis Molecular Epidemiology Spoligotyping MIRU Brazil. Ciências da Saúde Farmácia |
description |
Tuberculosis (TB) is an important public health problem worldwide and the leading cause of deaths by an infectious agent. The objective of this study was provide information about genetic diversity of Mycobacterium tuberculosis, isolated from patients with pulmonary TB attended at the Laboratório de Ensino e Pesquisa em Análises Clínicas (LEPAC), using Spoligotyping and MIRU-VNTR methodologies. The results were reported in the paper "Genotyping of Mycobacterium tuberculosis isolates from low endemic setting in south of Brazil". From the Spoligotyping and MIRU-VNTR patterns of M. tuberculosis isolates dendrograms were constructed to evaluate the relationship of the isolates. The results show that TB in our region predominantly develops from endogenous reactivation of a latent infection. It was also carried out a comparison of discriminatory power between the methodologies used (Spoligotyping and MIRU-VNTR), obtaining better discriminatory capacity of the isolates combining the two methodologies. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2018-04-06T19:45:55Z 2018-04-06T19:45:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.uem.br:8080/jspui/handle/1/1901 |
url |
http://repositorio.uem.br:8080/jspui/handle/1/1901 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá Brasil Departamento de Análises Clínicas Programa de Pós-Graduação em Biociências Aplicadas à Farmácia UEM Maringá, PR Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá Brasil Departamento de Análises Clínicas Programa de Pós-Graduação em Biociências Aplicadas à Farmácia UEM Maringá, PR Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
collection |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
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_version_ |
1813258645349072896 |