In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.

Detalhes bibliográficos
Autor(a) principal: Trento, Marcus Vinícius Cardoso
Data de Publicação: 2021
Outros Autores: Carapiá, Mateus Santos, César, Pedro Henrique Souza, Braga, Mariana Aparecida, Soares, Andreimar Martins, Marcussi, Silvana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016
Resumo: The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal.
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spelling In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal.The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal.Universidade Estadual De Maringá2021-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5701610.4025/actascibiolsci.v43i1.57016Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e57016Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e570161807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016/751375153155Copyright (c) 2021 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Trento, Marcus Vinícius Cardoso Carapiá, Mateus Santos César, Pedro Henrique Souza Braga, Mariana Aparecida Soares, Andreimar Martins Marcussi, Silvana2022-02-17T21:58:36Zoai:periodicos.uem.br/ojs:article/57016Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T21:58:36Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
title In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
spellingShingle In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
Trento, Marcus Vinícius Cardoso
snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.
snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.
title_short In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
title_full In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
title_fullStr In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
title_full_unstemmed In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
title_sort In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
author Trento, Marcus Vinícius Cardoso
author_facet Trento, Marcus Vinícius Cardoso
Carapiá, Mateus Santos
César, Pedro Henrique Souza
Braga, Mariana Aparecida
Soares, Andreimar Martins
Marcussi, Silvana
author_role author
author2 Carapiá, Mateus Santos
César, Pedro Henrique Souza
Braga, Mariana Aparecida
Soares, Andreimar Martins
Marcussi, Silvana
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Trento, Marcus Vinícius Cardoso
Carapiá, Mateus Santos
César, Pedro Henrique Souza
Braga, Mariana Aparecida
Soares, Andreimar Martins
Marcussi, Silvana
dc.subject.por.fl_str_mv snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.
snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.
topic snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.
snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.
description The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016
10.4025/actascibiolsci.v43i1.57016
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016
identifier_str_mv 10.4025/actascibiolsci.v43i1.57016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016/751375153155
dc.rights.driver.fl_str_mv Copyright (c) 2021 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e57016
Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e57016
1807-863X
1679-9283
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instname_str Universidade Estadual de Maringá (UEM)
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institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
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