In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016 |
Resumo: | The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal. |
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In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae.The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal.The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal.Universidade Estadual De Maringá2021-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5701610.4025/actascibiolsci.v43i1.57016Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e57016Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e570161807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016/751375153155Copyright (c) 2021 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Trento, Marcus Vinícius Cardoso Carapiá, Mateus Santos César, Pedro Henrique Souza Braga, Mariana Aparecida Soares, Andreimar Martins Marcussi, Silvana2022-02-17T21:58:36Zoai:periodicos.uem.br/ojs:article/57016Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T21:58:36Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. |
title |
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. |
spellingShingle |
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. Trento, Marcus Vinícius Cardoso snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae. snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae. |
title_short |
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. |
title_full |
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. |
title_fullStr |
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. |
title_full_unstemmed |
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. |
title_sort |
In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L. |
author |
Trento, Marcus Vinícius Cardoso |
author_facet |
Trento, Marcus Vinícius Cardoso Carapiá, Mateus Santos César, Pedro Henrique Souza Braga, Mariana Aparecida Soares, Andreimar Martins Marcussi, Silvana |
author_role |
author |
author2 |
Carapiá, Mateus Santos César, Pedro Henrique Souza Braga, Mariana Aparecida Soares, Andreimar Martins Marcussi, Silvana |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Trento, Marcus Vinícius Cardoso Carapiá, Mateus Santos César, Pedro Henrique Souza Braga, Mariana Aparecida Soares, Andreimar Martins Marcussi, Silvana |
dc.subject.por.fl_str_mv |
snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae. snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae. |
topic |
snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae. snake venoms; enzyme inhibitors; medicinal plants; Bignoniaceae. |
description |
The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016 10.4025/actascibiolsci.v43i1.57016 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016 |
identifier_str_mv |
10.4025/actascibiolsci.v43i1.57016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/57016/751375153155 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e57016 Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e57016 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317391062073344 |