The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs

Detalhes bibliográficos
Autor(a) principal: Martinazzo, Emanuela Garbin
Data de Publicação: 2015
Outros Autores: Perbini, Anelise Tessari, Bacarin, Marcos Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23263
Resumo: The mechanisms of photosynthetic electron transport can be elucidated by inhibition of electron flow through the use of specific substances that, when combined with the chlorophyll  chlorophyll a fluorescence emission was measured to investigate the effect of several inhibitors of the photosynthetic electron transport chain in canola leaf discs. Leaf discs were incubated in the dark for 2 hours in different solutions: (a) water – control; (b) DCMU at 500 µM; (c) phenanthroline at 10 mM; and (d) hydroxylamine at 10, 50, 100 and 200 mM. Similar effects were observed between DCMU and phenanthroline, the initial fluorescence value was altered, but not the maximum fluorescence, with the disappearance of the IP phase. Hydroxylamine interacted and inhibited the oxygen evolving complex and caused an imbalance between the rate of QA reduction by photosystem II and the rate of QA oxidation by photosystem I. 
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spelling The effect of inhibitors on photosynthetic electron transport chain in canola leaf discsDCMUhydroxylaminephenanthrolineoxygen evolving complexchlorophyll fluorescenceFisiologia VegetalThe mechanisms of photosynthetic electron transport can be elucidated by inhibition of electron flow through the use of specific substances that, when combined with the chlorophyll  chlorophyll a fluorescence emission was measured to investigate the effect of several inhibitors of the photosynthetic electron transport chain in canola leaf discs. Leaf discs were incubated in the dark for 2 hours in different solutions: (a) water – control; (b) DCMU at 500 µM; (c) phenanthroline at 10 mM; and (d) hydroxylamine at 10, 50, 100 and 200 mM. Similar effects were observed between DCMU and phenanthroline, the initial fluorescence value was altered, but not the maximum fluorescence, with the disappearance of the IP phase. Hydroxylamine interacted and inhibited the oxygen evolving complex and caused an imbalance between the rate of QA reduction by photosystem II and the rate of QA oxidation by photosystem I. Universidade Estadual De Maringá2015-08-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2326310.4025/actascibiolsci.v37i2.23263Acta Scientiarum. Biological Sciences; Vol 37 No 2 (2015); 159-167Acta Scientiarum. Biological Sciences; v. 37 n. 2 (2015); 159-1671807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23263/pdf_51Martinazzo, Emanuela GarbinPerbini, Anelise TessariBacarin, Marcos Antonioinfo:eu-repo/semantics/openAccess2015-08-06T15:38:50Zoai:periodicos.uem.br/ojs:article/23263Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2015-08-06T15:38:50Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs
title The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs
spellingShingle The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs
Martinazzo, Emanuela Garbin
DCMU
hydroxylamine
phenanthroline
oxygen evolving complex
chlorophyll fluorescence
Fisiologia Vegetal
title_short The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs
title_full The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs
title_fullStr The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs
title_full_unstemmed The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs
title_sort The effect of inhibitors on photosynthetic electron transport chain in canola leaf discs
author Martinazzo, Emanuela Garbin
author_facet Martinazzo, Emanuela Garbin
Perbini, Anelise Tessari
Bacarin, Marcos Antonio
author_role author
author2 Perbini, Anelise Tessari
Bacarin, Marcos Antonio
author2_role author
author
dc.contributor.author.fl_str_mv Martinazzo, Emanuela Garbin
Perbini, Anelise Tessari
Bacarin, Marcos Antonio
dc.subject.por.fl_str_mv DCMU
hydroxylamine
phenanthroline
oxygen evolving complex
chlorophyll fluorescence
Fisiologia Vegetal
topic DCMU
hydroxylamine
phenanthroline
oxygen evolving complex
chlorophyll fluorescence
Fisiologia Vegetal
description The mechanisms of photosynthetic electron transport can be elucidated by inhibition of electron flow through the use of specific substances that, when combined with the chlorophyll  chlorophyll a fluorescence emission was measured to investigate the effect of several inhibitors of the photosynthetic electron transport chain in canola leaf discs. Leaf discs were incubated in the dark for 2 hours in different solutions: (a) water – control; (b) DCMU at 500 µM; (c) phenanthroline at 10 mM; and (d) hydroxylamine at 10, 50, 100 and 200 mM. Similar effects were observed between DCMU and phenanthroline, the initial fluorescence value was altered, but not the maximum fluorescence, with the disappearance of the IP phase. Hydroxylamine interacted and inhibited the oxygen evolving complex and caused an imbalance between the rate of QA reduction by photosystem II and the rate of QA oxidation by photosystem I. 
publishDate 2015
dc.date.none.fl_str_mv 2015-08-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23263
10.4025/actascibiolsci.v37i2.23263
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23263
identifier_str_mv 10.4025/actascibiolsci.v37i2.23263
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23263/pdf_51
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 37 No 2 (2015); 159-167
Acta Scientiarum. Biological Sciences; v. 37 n. 2 (2015); 159-167
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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