Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region

Detalhes bibliográficos
Autor(a) principal: Rosa, Daniel Dias
Data de Publicação: 2008
Outros Autores: Marque, Janaina Marianno de, Fenille, Roseli Chela, Souza, Nilton Luiz de, Kuramae, Eiko Eurya
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2033
Resumo: Phylogenetic analysis through morphological and cultural traits is considered inconsistent and hard to be scientifically accepted once there is no way to establish the direction of evolution on morphological traits. Molecular markers are suitable for phylogenetic analysis. The sequencing of ITS1 and ITS2 (internal transcribed spacers) regions and of 5.8S gene from ribosomal DNA were used to estimate the genetic variation and distance of 10 Phytophthora capsici isolates. The amount of genetic variation amongst isolated P. capsici from distinct regions of São Paulo State was 0.1 to 1.6 and 0.1 to 1.1% at ITS1 and ITS2, respectively, and a phylogenetic distance of about 78.5% between P. capsici and Phytophthora spp. was observed
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spelling Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA regionAnálise filogenética de Phytophthora capsici Leonian do Estado de São Paulo baseada na seqüência de nucleotídeos da região ITS-5.8S rDNA - DOI: 10.4025/actascibiolsci.v25i2.2033DNA ribossomalITSfFilogeniaPhytophthora sp2.01.00.00-0 Biologia GeralPhylogenetic analysis through morphological and cultural traits is considered inconsistent and hard to be scientifically accepted once there is no way to establish the direction of evolution on morphological traits. Molecular markers are suitable for phylogenetic analysis. The sequencing of ITS1 and ITS2 (internal transcribed spacers) regions and of 5.8S gene from ribosomal DNA were used to estimate the genetic variation and distance of 10 Phytophthora capsici isolates. The amount of genetic variation amongst isolated P. capsici from distinct regions of São Paulo State was 0.1 to 1.6 and 0.1 to 1.1% at ITS1 and ITS2, respectively, and a phylogenetic distance of about 78.5% between P. capsici and Phytophthora spp. was observedAnálise filogenética através de características morfológicas e culturais é considerada inconsistente e de difícil aceitação cientiífica, uma vez que não há como estabelecer a direção da evolução de caracteres morfológicos. Os mMarcadores moleculares são mais apropriados para análises filogenéticas. O seqüeênciamento das regiões ITS1 e ITS2 (Internal Transcribed Spacer) e o gene 5.8S rDNA forami utilizados para estimar a variação e a distância genética entre 10 isolados de Phytophthora capsici. A quantidade de variação genética entre os isolados de P. capsici de diferentes regiões do estadoEstado de São Paulo foi de 0,1% a 1,6% e de 0,1% a 1,1% nas regiões ITS1 e ITS2, respectivamente, e uma distância filogenética de 78,5% entre os isolados de P. capsici e Phytophthora sppUniversidade Estadual De Maringá2008-04-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/203310.4025/actascibiolsci.v25i2.2033Acta Scientiarum. Biological Sciences; Vol 25 No 2 (2003); 429-434Acta Scientiarum. Biological Sciences; v. 25 n. 2 (2003); 429-4341807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2033/1509Rosa, Daniel DiasMarque, Janaina Marianno deFenille, Roseli ChelaSouza, Nilton Luiz deKuramae, Eiko Euryainfo:eu-repo/semantics/openAccess2022-11-23T17:33:18Zoai:periodicos.uem.br/ojs:article/2033Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:18Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region
Análise filogenética de Phytophthora capsici Leonian do Estado de São Paulo baseada na seqüência de nucleotídeos da região ITS-5.8S rDNA - DOI: 10.4025/actascibiolsci.v25i2.2033
title Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region
spellingShingle Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region
Rosa, Daniel Dias
DNA ribossomal
ITS
fFilogenia
Phytophthora sp
2.01.00.00-0 Biologia Geral
title_short Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region
title_full Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region
title_fullStr Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region
title_full_unstemmed Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region
title_sort Phylogenetic analysis of Phytophthora capsici Leonian in the state of São Paulo based on the nucleotides sequence of ITS-5.8S rDNA region
author Rosa, Daniel Dias
author_facet Rosa, Daniel Dias
Marque, Janaina Marianno de
Fenille, Roseli Chela
Souza, Nilton Luiz de
Kuramae, Eiko Eurya
author_role author
author2 Marque, Janaina Marianno de
Fenille, Roseli Chela
Souza, Nilton Luiz de
Kuramae, Eiko Eurya
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rosa, Daniel Dias
Marque, Janaina Marianno de
Fenille, Roseli Chela
Souza, Nilton Luiz de
Kuramae, Eiko Eurya
dc.subject.por.fl_str_mv DNA ribossomal
ITS
fFilogenia
Phytophthora sp
2.01.00.00-0 Biologia Geral
topic DNA ribossomal
ITS
fFilogenia
Phytophthora sp
2.01.00.00-0 Biologia Geral
description Phylogenetic analysis through morphological and cultural traits is considered inconsistent and hard to be scientifically accepted once there is no way to establish the direction of evolution on morphological traits. Molecular markers are suitable for phylogenetic analysis. The sequencing of ITS1 and ITS2 (internal transcribed spacers) regions and of 5.8S gene from ribosomal DNA were used to estimate the genetic variation and distance of 10 Phytophthora capsici isolates. The amount of genetic variation amongst isolated P. capsici from distinct regions of São Paulo State was 0.1 to 1.6 and 0.1 to 1.1% at ITS1 and ITS2, respectively, and a phylogenetic distance of about 78.5% between P. capsici and Phytophthora spp. was observed
publishDate 2008
dc.date.none.fl_str_mv 2008-04-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2033
10.4025/actascibiolsci.v25i2.2033
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2033
identifier_str_mv 10.4025/actascibiolsci.v25i2.2033
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2033/1509
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 25 No 2 (2003); 429-434
Acta Scientiarum. Biological Sciences; v. 25 n. 2 (2003); 429-434
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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