Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers

Detalhes bibliográficos
Autor(a) principal: Luz, Gizele de Andrade
Data de Publicação: 2020
Outros Autores: Santos, Jailson de Araújo, Oliveira, Kelly Pires de, Martins, Pâmela Ponce, Valente, Sérgio Emílio dos Santos, Maia, Maria Clideana Cabral, Lima, Paulo Sarmanho da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606
Resumo:  The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species.
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spelling Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markersGenetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markersApocynaceae; genetic structure; genetic variability; ISSR markers.Apocynaceae; genetic structure; genetic variability; ISSR markers. The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species. The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species.Universidade Estadual De Maringá2020-08-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5360610.4025/actascibiolsci.v42i1.53606Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e53606Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e536061807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606/751375150629Copyright (c) 2020 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLuz, Gizele de Andrade Santos, Jailson de Araújo Oliveira, Kelly Pires de Martins, Pâmela Ponce Valente, Sérgio Emílio dos Santos Maia, Maria Clideana Cabral Lima, Paulo Sarmanho da Costa2020-11-16T16:25:43Zoai:periodicos.uem.br/ojs:article/53606Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2020-11-16T16:25:43Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
title Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
spellingShingle Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
Luz, Gizele de Andrade
Apocynaceae; genetic structure; genetic variability; ISSR markers.
Apocynaceae; genetic structure; genetic variability; ISSR markers.
title_short Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
title_full Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
title_fullStr Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
title_full_unstemmed Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
title_sort Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
author Luz, Gizele de Andrade
author_facet Luz, Gizele de Andrade
Santos, Jailson de Araújo
Oliveira, Kelly Pires de
Martins, Pâmela Ponce
Valente, Sérgio Emílio dos Santos
Maia, Maria Clideana Cabral
Lima, Paulo Sarmanho da Costa
author_role author
author2 Santos, Jailson de Araújo
Oliveira, Kelly Pires de
Martins, Pâmela Ponce
Valente, Sérgio Emílio dos Santos
Maia, Maria Clideana Cabral
Lima, Paulo Sarmanho da Costa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Luz, Gizele de Andrade
Santos, Jailson de Araújo
Oliveira, Kelly Pires de
Martins, Pâmela Ponce
Valente, Sérgio Emílio dos Santos
Maia, Maria Clideana Cabral
Lima, Paulo Sarmanho da Costa
dc.subject.por.fl_str_mv Apocynaceae; genetic structure; genetic variability; ISSR markers.
Apocynaceae; genetic structure; genetic variability; ISSR markers.
topic Apocynaceae; genetic structure; genetic variability; ISSR markers.
Apocynaceae; genetic structure; genetic variability; ISSR markers.
description  The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606
10.4025/actascibiolsci.v42i1.53606
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606
identifier_str_mv 10.4025/actascibiolsci.v42i1.53606
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606/751375150629
dc.rights.driver.fl_str_mv Copyright (c) 2020 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e53606
Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e53606
1807-863X
1679-9283
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instacron_str UEM
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reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
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