Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606 |
Resumo: | The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species. |
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Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markersGenetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markersApocynaceae; genetic structure; genetic variability; ISSR markers.Apocynaceae; genetic structure; genetic variability; ISSR markers. The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species. The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species.Universidade Estadual De Maringá2020-08-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5360610.4025/actascibiolsci.v42i1.53606Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e53606Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e536061807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606/751375150629Copyright (c) 2020 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLuz, Gizele de Andrade Santos, Jailson de Araújo Oliveira, Kelly Pires de Martins, Pâmela Ponce Valente, Sérgio Emílio dos Santos Maia, Maria Clideana Cabral Lima, Paulo Sarmanho da Costa2020-11-16T16:25:43Zoai:periodicos.uem.br/ojs:article/53606Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2020-11-16T16:25:43Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers |
title |
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers |
spellingShingle |
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers Luz, Gizele de Andrade Apocynaceae; genetic structure; genetic variability; ISSR markers. Apocynaceae; genetic structure; genetic variability; ISSR markers. |
title_short |
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers |
title_full |
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers |
title_fullStr |
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers |
title_full_unstemmed |
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers |
title_sort |
Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers |
author |
Luz, Gizele de Andrade |
author_facet |
Luz, Gizele de Andrade Santos, Jailson de Araújo Oliveira, Kelly Pires de Martins, Pâmela Ponce Valente, Sérgio Emílio dos Santos Maia, Maria Clideana Cabral Lima, Paulo Sarmanho da Costa |
author_role |
author |
author2 |
Santos, Jailson de Araújo Oliveira, Kelly Pires de Martins, Pâmela Ponce Valente, Sérgio Emílio dos Santos Maia, Maria Clideana Cabral Lima, Paulo Sarmanho da Costa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Luz, Gizele de Andrade Santos, Jailson de Araújo Oliveira, Kelly Pires de Martins, Pâmela Ponce Valente, Sérgio Emílio dos Santos Maia, Maria Clideana Cabral Lima, Paulo Sarmanho da Costa |
dc.subject.por.fl_str_mv |
Apocynaceae; genetic structure; genetic variability; ISSR markers. Apocynaceae; genetic structure; genetic variability; ISSR markers. |
topic |
Apocynaceae; genetic structure; genetic variability; ISSR markers. Apocynaceae; genetic structure; genetic variability; ISSR markers. |
description |
The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606 10.4025/actascibiolsci.v42i1.53606 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606 |
identifier_str_mv |
10.4025/actascibiolsci.v42i1.53606 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53606/751375150629 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e53606 Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e53606 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317397885157376 |