Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29479 |
Resumo: | The production of propionic acid using skim serum by-products of the dairy industry was investigated, and the influence of inoculum concentration of Propionibacterium freudenreichii PS-1 and its time of inoculation for the production of propionic acid was evaluated using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii. Initially, the adjustment of propionic bacteria to higher concentrations of propionic acid was tested and kinetic parameters were determined for growth. A central composite rotational design (CCRD) with 11 experiments was carried out, using different inoculum concentrations of P. freudenreichii, which were added to the cultures of lactic bacteria at different times. The maximum specific growth rate for P. freudenreichii decreased from 0.11 to 0.03 hour-1and the growth time increased from 32h to 100h when concentration of propionic acid in lactate broth increased to 0.25%. The best result for propionic acid production was 3.78 g L-1, inoculating 5.108 CFU mL-1 of propionic bacteria after 6 h. For lactose consumption, the best conditions were inoculum concentration above 5.108 CFU mL-1 and inoculation time of P. freudenreichii after 6 hours. Therefore, it was demonstrated that skimmed whey can be used as a renewable, low-cost raw material for propionic acid production by mixed culture. |
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Acta scientiarum. Technology (Online) |
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Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1skimmed wheylactic bacteriapropionic fermentationorganic acids.5.07.02.00-9 TECNOLOGIA DE ALIMENTOS The production of propionic acid using skim serum by-products of the dairy industry was investigated, and the influence of inoculum concentration of Propionibacterium freudenreichii PS-1 and its time of inoculation for the production of propionic acid was evaluated using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii. Initially, the adjustment of propionic bacteria to higher concentrations of propionic acid was tested and kinetic parameters were determined for growth. A central composite rotational design (CCRD) with 11 experiments was carried out, using different inoculum concentrations of P. freudenreichii, which were added to the cultures of lactic bacteria at different times. The maximum specific growth rate for P. freudenreichii decreased from 0.11 to 0.03 hour-1and the growth time increased from 32h to 100h when concentration of propionic acid in lactate broth increased to 0.25%. The best result for propionic acid production was 3.78 g L-1, inoculating 5.108 CFU mL-1 of propionic bacteria after 6 h. For lactose consumption, the best conditions were inoculum concentration above 5.108 CFU mL-1 and inoculation time of P. freudenreichii after 6 hours. Therefore, it was demonstrated that skimmed whey can be used as a renewable, low-cost raw material for propionic acid production by mixed culture. Universidade Estadual De Maringá2017-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionFERMENTAÇÃOapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/2947910.4025/actascitechnol.v39i5.29479Acta Scientiarum. Technology; Vol 39 (2017): Special Supplement; 543-550Acta Scientiarum. Technology; v. 39 (2017): Special Supplement; 543-5501806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29479/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29479/751375144251Copyright (c) 2017 Acta Scientiarum. Technologyinfo:eu-repo/semantics/openAccessNgome, Moisés TomásAlves, José Guilherme Lembi FerreiraPiccoli, Roberta HilsdorfDomingo, Elisângela de CarmoPinto, Sheyla AnáliaBernal, Olga Lucía Mondragón2018-01-29T10:38:03Zoai:periodicos.uem.br/ojs:article/29479Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2018-01-29T10:38:03Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1 |
title |
Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1 |
spellingShingle |
Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1 Ngome, Moisés Tomás skimmed whey lactic bacteria propionic fermentation organic acids. 5.07.02.00-9 TECNOLOGIA DE ALIMENTOS |
title_short |
Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1 |
title_full |
Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1 |
title_fullStr |
Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1 |
title_full_unstemmed |
Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1 |
title_sort |
Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1 |
author |
Ngome, Moisés Tomás |
author_facet |
Ngome, Moisés Tomás Alves, José Guilherme Lembi Ferreira Piccoli, Roberta Hilsdorf Domingo, Elisângela de Carmo Pinto, Sheyla Anália Bernal, Olga Lucía Mondragón |
author_role |
author |
author2 |
Alves, José Guilherme Lembi Ferreira Piccoli, Roberta Hilsdorf Domingo, Elisângela de Carmo Pinto, Sheyla Anália Bernal, Olga Lucía Mondragón |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ngome, Moisés Tomás Alves, José Guilherme Lembi Ferreira Piccoli, Roberta Hilsdorf Domingo, Elisângela de Carmo Pinto, Sheyla Anália Bernal, Olga Lucía Mondragón |
dc.subject.por.fl_str_mv |
skimmed whey lactic bacteria propionic fermentation organic acids. 5.07.02.00-9 TECNOLOGIA DE ALIMENTOS |
topic |
skimmed whey lactic bacteria propionic fermentation organic acids. 5.07.02.00-9 TECNOLOGIA DE ALIMENTOS |
description |
The production of propionic acid using skim serum by-products of the dairy industry was investigated, and the influence of inoculum concentration of Propionibacterium freudenreichii PS-1 and its time of inoculation for the production of propionic acid was evaluated using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii. Initially, the adjustment of propionic bacteria to higher concentrations of propionic acid was tested and kinetic parameters were determined for growth. A central composite rotational design (CCRD) with 11 experiments was carried out, using different inoculum concentrations of P. freudenreichii, which were added to the cultures of lactic bacteria at different times. The maximum specific growth rate for P. freudenreichii decreased from 0.11 to 0.03 hour-1and the growth time increased from 32h to 100h when concentration of propionic acid in lactate broth increased to 0.25%. The best result for propionic acid production was 3.78 g L-1, inoculating 5.108 CFU mL-1 of propionic bacteria after 6 h. For lactose consumption, the best conditions were inoculum concentration above 5.108 CFU mL-1 and inoculation time of P. freudenreichii after 6 hours. Therefore, it was demonstrated that skimmed whey can be used as a renewable, low-cost raw material for propionic acid production by mixed culture. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion FERMENTAÇÃO |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29479 10.4025/actascitechnol.v39i5.29479 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29479 |
identifier_str_mv |
10.4025/actascitechnol.v39i5.29479 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29479/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/29479/751375144251 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Acta Scientiarum. Technology info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Acta Scientiarum. Technology |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 39 (2017): Special Supplement; 543-550 Acta Scientiarum. Technology; v. 39 (2017): Special Supplement; 543-550 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
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1799315335986282496 |