Medium composition and Amazonian oils for lipase production by Candida viswanathii
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/35088 |
Resumo: | Lipases have been successfully produced under submerged cultivation conditions using olive oil as carbon source and complex culture media. The aims of this study were to evaluate lipase production by Candida viswanathii in submerged cultivation with different culture medium compositions, replace olive oil with plant oils from the Amazonian region, and assess the effect of nitrogen sources. By using olive oil as the sole carbon source, C. viswanathii produced high levels of biomass and lipase with minimal nutritional requirements (potassium, magnesium, and yeast extract). Plant oils from the Amazonian region supported cell growth, and pequi oil was the best inducer for lipase production (1.66 U mL-1). In comparison with inorganic sources, organic nitrogen sources induced the highest levels of lipase production. Typical microbial growth and lipase production were observed using pequi oil and peptone as a nitrogen source. The selected conditions resulted in good cell growth (YX/S = 1.25 g g-1) and lipase production (YP/S= 118.10 U g-1) after 60 hours of cultivation. In conclusion, the fermentation parameters indicated that Amazonian oils are promising for lipase production by C. viswanathii, and oils modified by this enzyme can be used in the food and cosmetic industries. |
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Medium composition and Amazonian oils for lipase production by Candida viswanathiicarbon sourcesenzyme productionfermentation parameters.Microbiologia Industrial e de Fermentação Lipases have been successfully produced under submerged cultivation conditions using olive oil as carbon source and complex culture media. The aims of this study were to evaluate lipase production by Candida viswanathii in submerged cultivation with different culture medium compositions, replace olive oil with plant oils from the Amazonian region, and assess the effect of nitrogen sources. By using olive oil as the sole carbon source, C. viswanathii produced high levels of biomass and lipase with minimal nutritional requirements (potassium, magnesium, and yeast extract). Plant oils from the Amazonian region supported cell growth, and pequi oil was the best inducer for lipase production (1.66 U mL-1). In comparison with inorganic sources, organic nitrogen sources induced the highest levels of lipase production. Typical microbial growth and lipase production were observed using pequi oil and peptone as a nitrogen source. The selected conditions resulted in good cell growth (YX/S = 1.25 g g-1) and lipase production (YP/S= 118.10 U g-1) after 60 hours of cultivation. In conclusion, the fermentation parameters indicated that Amazonian oils are promising for lipase production by C. viswanathii, and oils modified by this enzyme can be used in the food and cosmetic industries. Universidade Estadual De Maringá2018-04-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionfermentação; enzimas;application/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/3508810.4025/actascitechnol.v40i1.35088Acta Scientiarum. Technology; Vol 40 (2018): Publicação Contínua; e35088Acta Scientiarum. Technology; v. 40 (2018): Publicação Contínua; e350881806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/35088/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/35088/751375145562Copyright (c) 2018 Acta Scientiarum. Technologyinfo:eu-repo/semantics/openAccessGomes, Nayane BarrosoDias, Kleydiane BragaTeixeira, Mayra Ferreira NettoSantos, Claudia Cristina Auler do AmaralAlmeida, Alex Fernando de2019-07-17T11:53:47Zoai:periodicos.uem.br/ojs:article/35088Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2019-07-17T11:53:47Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Medium composition and Amazonian oils for lipase production by Candida viswanathii |
title |
Medium composition and Amazonian oils for lipase production by Candida viswanathii |
spellingShingle |
Medium composition and Amazonian oils for lipase production by Candida viswanathii Gomes, Nayane Barroso carbon sources enzyme production fermentation parameters. Microbiologia Industrial e de Fermentação |
title_short |
Medium composition and Amazonian oils for lipase production by Candida viswanathii |
title_full |
Medium composition and Amazonian oils for lipase production by Candida viswanathii |
title_fullStr |
Medium composition and Amazonian oils for lipase production by Candida viswanathii |
title_full_unstemmed |
Medium composition and Amazonian oils for lipase production by Candida viswanathii |
title_sort |
Medium composition and Amazonian oils for lipase production by Candida viswanathii |
author |
Gomes, Nayane Barroso |
author_facet |
Gomes, Nayane Barroso Dias, Kleydiane Braga Teixeira, Mayra Ferreira Netto Santos, Claudia Cristina Auler do Amaral Almeida, Alex Fernando de |
author_role |
author |
author2 |
Dias, Kleydiane Braga Teixeira, Mayra Ferreira Netto Santos, Claudia Cristina Auler do Amaral Almeida, Alex Fernando de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gomes, Nayane Barroso Dias, Kleydiane Braga Teixeira, Mayra Ferreira Netto Santos, Claudia Cristina Auler do Amaral Almeida, Alex Fernando de |
dc.subject.por.fl_str_mv |
carbon sources enzyme production fermentation parameters. Microbiologia Industrial e de Fermentação |
topic |
carbon sources enzyme production fermentation parameters. Microbiologia Industrial e de Fermentação |
description |
Lipases have been successfully produced under submerged cultivation conditions using olive oil as carbon source and complex culture media. The aims of this study were to evaluate lipase production by Candida viswanathii in submerged cultivation with different culture medium compositions, replace olive oil with plant oils from the Amazonian region, and assess the effect of nitrogen sources. By using olive oil as the sole carbon source, C. viswanathii produced high levels of biomass and lipase with minimal nutritional requirements (potassium, magnesium, and yeast extract). Plant oils from the Amazonian region supported cell growth, and pequi oil was the best inducer for lipase production (1.66 U mL-1). In comparison with inorganic sources, organic nitrogen sources induced the highest levels of lipase production. Typical microbial growth and lipase production were observed using pequi oil and peptone as a nitrogen source. The selected conditions resulted in good cell growth (YX/S = 1.25 g g-1) and lipase production (YP/S= 118.10 U g-1) after 60 hours of cultivation. In conclusion, the fermentation parameters indicated that Amazonian oils are promising for lipase production by C. viswanathii, and oils modified by this enzyme can be used in the food and cosmetic industries. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion fermentação; enzimas; |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/35088 10.4025/actascitechnol.v40i1.35088 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/35088 |
identifier_str_mv |
10.4025/actascitechnol.v40i1.35088 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/35088/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/35088/751375145562 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Acta Scientiarum. Technology info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Acta Scientiarum. Technology |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 40 (2018): Publicação Contínua; e35088 Acta Scientiarum. Technology; v. 40 (2018): Publicação Contínua; e35088 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315336405712896 |