Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/16709 |
Resumo: | Studies on biopolymers from macroalgae suggested sulfated polysaccharides (SPs) as research agents to investigate events related to haemostasis. Caulerpa cupressoides var. lycopodium is a marine green alga containing three SPs fractions (SP1, SP2 and SP3). SP2 had anticoagulant (in vitro) and anti- and prothrombotic (in vivo) actions; however, its effect on the coagulation system is not fully understood. This study aimed to determine the infrared (IR) spectroscopy, chemical composition (CC), elemental microanalysis (EM), molecular weight (MW) and the effect on coagulation proteases of SP2. The presence of sulfate ester, galactose-6-sulfate, uronic acid and glycoside linkages for IR spectrum; contents of sulfate (28%), total sugars (40%) and uronic acids (7.18%) for CC; and content of carbon (21.98%), sulfate (4.27%), nitrogen (1.3%) and hydrogen (4.86%) for EM were obtained. The average molecular weights of four different SPs (SP-1, SP-2, SP-3 and SP-4) subfractions from the SP2 ranged from ~ 8 to >100 kDa. SP2 was tested on coagulation proteases (thrombin and factor Xa) in the presence of antithrombin (AT) and heparin cofactor II (HCII) using human plasma, being both thrombin and factor Xa target proteases inhibited, but requiring a concentration of about 2.5-fold higher of HCII than the thrombin inactivation by AT. |
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Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709Caulerpaceaepolysulfatedchemical analysiscoagulationmodulator action2.08.01.00-9Studies on biopolymers from macroalgae suggested sulfated polysaccharides (SPs) as research agents to investigate events related to haemostasis. Caulerpa cupressoides var. lycopodium is a marine green alga containing three SPs fractions (SP1, SP2 and SP3). SP2 had anticoagulant (in vitro) and anti- and prothrombotic (in vivo) actions; however, its effect on the coagulation system is not fully understood. This study aimed to determine the infrared (IR) spectroscopy, chemical composition (CC), elemental microanalysis (EM), molecular weight (MW) and the effect on coagulation proteases of SP2. The presence of sulfate ester, galactose-6-sulfate, uronic acid and glycoside linkages for IR spectrum; contents of sulfate (28%), total sugars (40%) and uronic acids (7.18%) for CC; and content of carbon (21.98%), sulfate (4.27%), nitrogen (1.3%) and hydrogen (4.86%) for EM were obtained. The average molecular weights of four different SPs (SP-1, SP-2, SP-3 and SP-4) subfractions from the SP2 ranged from ~ 8 to >100 kDa. SP2 was tested on coagulation proteases (thrombin and factor Xa) in the presence of antithrombin (AT) and heparin cofactor II (HCII) using human plasma, being both thrombin and factor Xa target proteases inhibited, but requiring a concentration of about 2.5-fold higher of HCII than the thrombin inactivation by AT. Universidade Estadual De Maringá2013-05-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionanálise moduladoraapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1670910.4025/actascitechnol.v35i4.16709Acta Scientiarum. Technology; Vol 35 No 4 (2013); 611-619Acta Scientiarum. Technology; v. 35 n. 4 (2013); 611-6191806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/16709/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/16709/pdf_1Rodrigues, José Ariévilo GurgelNeto, Érico MouraTeixeira, Lisandra Antônia CastroPaula, Regina Célia Monteiro deMourão, Paulo Antônio de SouzaBenevides, Norma Maria Barrosinfo:eu-repo/semantics/openAccess2024-05-17T13:03:31Zoai:periodicos.uem.br/ojs:article/16709Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:03:31Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709 |
title |
Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709 |
spellingShingle |
Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709 Rodrigues, José Ariévilo Gurgel Caulerpaceae polysulfated chemical analysis coagulation modulator action 2.08.01.00-9 |
title_short |
Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709 |
title_full |
Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709 |
title_fullStr |
Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709 |
title_full_unstemmed |
Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709 |
title_sort |
Structural features and inactivation of coagulation proteases of a sulfated polysaccharidic fraction from Caulerpa cupressoides var. lycopodium (Caulerpaceae, Chlorophyta) - doi: 10.4025/actascitechnol.v35i4.16709 |
author |
Rodrigues, José Ariévilo Gurgel |
author_facet |
Rodrigues, José Ariévilo Gurgel Neto, Érico Moura Teixeira, Lisandra Antônia Castro Paula, Regina Célia Monteiro de Mourão, Paulo Antônio de Souza Benevides, Norma Maria Barros |
author_role |
author |
author2 |
Neto, Érico Moura Teixeira, Lisandra Antônia Castro Paula, Regina Célia Monteiro de Mourão, Paulo Antônio de Souza Benevides, Norma Maria Barros |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, José Ariévilo Gurgel Neto, Érico Moura Teixeira, Lisandra Antônia Castro Paula, Regina Célia Monteiro de Mourão, Paulo Antônio de Souza Benevides, Norma Maria Barros |
dc.subject.por.fl_str_mv |
Caulerpaceae polysulfated chemical analysis coagulation modulator action 2.08.01.00-9 |
topic |
Caulerpaceae polysulfated chemical analysis coagulation modulator action 2.08.01.00-9 |
description |
Studies on biopolymers from macroalgae suggested sulfated polysaccharides (SPs) as research agents to investigate events related to haemostasis. Caulerpa cupressoides var. lycopodium is a marine green alga containing three SPs fractions (SP1, SP2 and SP3). SP2 had anticoagulant (in vitro) and anti- and prothrombotic (in vivo) actions; however, its effect on the coagulation system is not fully understood. This study aimed to determine the infrared (IR) spectroscopy, chemical composition (CC), elemental microanalysis (EM), molecular weight (MW) and the effect on coagulation proteases of SP2. The presence of sulfate ester, galactose-6-sulfate, uronic acid and glycoside linkages for IR spectrum; contents of sulfate (28%), total sugars (40%) and uronic acids (7.18%) for CC; and content of carbon (21.98%), sulfate (4.27%), nitrogen (1.3%) and hydrogen (4.86%) for EM were obtained. The average molecular weights of four different SPs (SP-1, SP-2, SP-3 and SP-4) subfractions from the SP2 ranged from ~ 8 to >100 kDa. SP2 was tested on coagulation proteases (thrombin and factor Xa) in the presence of antithrombin (AT) and heparin cofactor II (HCII) using human plasma, being both thrombin and factor Xa target proteases inhibited, but requiring a concentration of about 2.5-fold higher of HCII than the thrombin inactivation by AT. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion análise moduladora |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/16709 10.4025/actascitechnol.v35i4.16709 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/16709 |
identifier_str_mv |
10.4025/actascitechnol.v35i4.16709 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/16709/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/16709/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 35 No 4 (2013); 611-619 Acta Scientiarum. Technology; v. 35 n. 4 (2013); 611-619 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
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1799315334950289408 |