Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/15365 |
Resumo: | Caulerpa cupressoides var. lycopodium (Chlorophyta) contains three sulfated polysaccharides (SPs) fractions (SP1, SP2 and SP3); but, their pharmacological properties have been limited. We investigated the antinociceptive activity of non-anticoagulant fraction (SP1) and then its acute toxicological study in male Swiss mice was performed. Animals (19-25 g) received i.v. SP1 30 min. prior to injection 0.8% acetic acid (10 mL kg-1, i.p.); 1% formalin (20 µL, i.pl.) or were subjected to thermal stimuli. Open-field test was also performed. Mice were treated i.p. with SP1 or 0.9% saline (0.1 mL 10 g-1) for 72h. On the 4th day, the animals were anesthetized and sacrificed in order to collect blood and organs. SP1 (3, 9 or 27 mg kg-1) reduced (p < 0.05) the number of writhes induced by acetic acid by 44.21, 47.72 and 90.87%, respectively. SP1 inhibited (p < 0.05) the second phase of the formalin test, without antinociceptive effect in the hot-plate test, suggesting that its analgesic action occurs through of peripheral mechanisms. SP1 did not modify the locomotor activity. SP1 (27 mg kg-1) did not cause hepatic or renal dysfunctions, but affected the spleen of animals (p < 0.05). Therefore, SP1 has analgesic action with high tolerance by the animals, presenting its potential applicability in pain conditions. |
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Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365green algasulfated sugarsnociceptionbiochemical analysis2.08.01.00-9Caulerpa cupressoides var. lycopodium (Chlorophyta) contains three sulfated polysaccharides (SPs) fractions (SP1, SP2 and SP3); but, their pharmacological properties have been limited. We investigated the antinociceptive activity of non-anticoagulant fraction (SP1) and then its acute toxicological study in male Swiss mice was performed. Animals (19-25 g) received i.v. SP1 30 min. prior to injection 0.8% acetic acid (10 mL kg-1, i.p.); 1% formalin (20 µL, i.pl.) or were subjected to thermal stimuli. Open-field test was also performed. Mice were treated i.p. with SP1 or 0.9% saline (0.1 mL 10 g-1) for 72h. On the 4th day, the animals were anesthetized and sacrificed in order to collect blood and organs. SP1 (3, 9 or 27 mg kg-1) reduced (p < 0.05) the number of writhes induced by acetic acid by 44.21, 47.72 and 90.87%, respectively. SP1 inhibited (p < 0.05) the second phase of the formalin test, without antinociceptive effect in the hot-plate test, suggesting that its analgesic action occurs through of peripheral mechanisms. SP1 did not modify the locomotor activity. SP1 (27 mg kg-1) did not cause hepatic or renal dysfunctions, but affected the spleen of animals (p < 0.05). Therefore, SP1 has analgesic action with high tolerance by the animals, presenting its potential applicability in pain conditions. Universidade Estadual De Maringá2013-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBioprospecção de agentes farmacológicosapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1536510.4025/actascitechnol.v35i3.15365Acta Scientiarum. Technology; Vol 35 No 3 (2013); 417-425Acta Scientiarum. Technology; v. 35 n. 3 (2013); 417-4251806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/15365/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/15365/pdf_1Rodrigues, José Ariévilo GurgelVanderlei, Edfranck de Sousa OliveiraQuinderé, Ana Luíza GomesMonteiro, Valdécio SilvanoVasconcelos, Silvânia Maria Mendes deBenevides, Norma Maria Barrosinfo:eu-repo/semantics/openAccess2024-05-17T13:03:28Zoai:periodicos.uem.br/ojs:article/15365Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:03:28Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365 |
title |
Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365 |
spellingShingle |
Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365 Rodrigues, José Ariévilo Gurgel green alga sulfated sugars nociception biochemical analysis 2.08.01.00-9 |
title_short |
Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365 |
title_full |
Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365 |
title_fullStr |
Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365 |
title_full_unstemmed |
Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365 |
title_sort |
Antinociceptive activity and acute toxicological study of a novel sulfated polysaccharide from Caulerpa cupressoides var. lycopodium (Chlorophyta) in Swiss mice - doi: 10.4025/actascitechnol.v35i3.15365 |
author |
Rodrigues, José Ariévilo Gurgel |
author_facet |
Rodrigues, José Ariévilo Gurgel Vanderlei, Edfranck de Sousa Oliveira Quinderé, Ana Luíza Gomes Monteiro, Valdécio Silvano Vasconcelos, Silvânia Maria Mendes de Benevides, Norma Maria Barros |
author_role |
author |
author2 |
Vanderlei, Edfranck de Sousa Oliveira Quinderé, Ana Luíza Gomes Monteiro, Valdécio Silvano Vasconcelos, Silvânia Maria Mendes de Benevides, Norma Maria Barros |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, José Ariévilo Gurgel Vanderlei, Edfranck de Sousa Oliveira Quinderé, Ana Luíza Gomes Monteiro, Valdécio Silvano Vasconcelos, Silvânia Maria Mendes de Benevides, Norma Maria Barros |
dc.subject.por.fl_str_mv |
green alga sulfated sugars nociception biochemical analysis 2.08.01.00-9 |
topic |
green alga sulfated sugars nociception biochemical analysis 2.08.01.00-9 |
description |
Caulerpa cupressoides var. lycopodium (Chlorophyta) contains three sulfated polysaccharides (SPs) fractions (SP1, SP2 and SP3); but, their pharmacological properties have been limited. We investigated the antinociceptive activity of non-anticoagulant fraction (SP1) and then its acute toxicological study in male Swiss mice was performed. Animals (19-25 g) received i.v. SP1 30 min. prior to injection 0.8% acetic acid (10 mL kg-1, i.p.); 1% formalin (20 µL, i.pl.) or were subjected to thermal stimuli. Open-field test was also performed. Mice were treated i.p. with SP1 or 0.9% saline (0.1 mL 10 g-1) for 72h. On the 4th day, the animals were anesthetized and sacrificed in order to collect blood and organs. SP1 (3, 9 or 27 mg kg-1) reduced (p < 0.05) the number of writhes induced by acetic acid by 44.21, 47.72 and 90.87%, respectively. SP1 inhibited (p < 0.05) the second phase of the formalin test, without antinociceptive effect in the hot-plate test, suggesting that its analgesic action occurs through of peripheral mechanisms. SP1 did not modify the locomotor activity. SP1 (27 mg kg-1) did not cause hepatic or renal dysfunctions, but affected the spleen of animals (p < 0.05). Therefore, SP1 has analgesic action with high tolerance by the animals, presenting its potential applicability in pain conditions. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bioprospecção de agentes farmacológicos |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/15365 10.4025/actascitechnol.v35i3.15365 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/15365 |
identifier_str_mv |
10.4025/actascitechnol.v35i3.15365 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/15365/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/15365/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 35 No 3 (2013); 417-425 Acta Scientiarum. Technology; v. 35 n. 3 (2013); 417-425 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315334434390016 |