Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201 |
Resumo: | Biochemical analyses are important tools for discovering new bioactive compounds for medical clinic. This study aimed at isolating iota-carrageenans (ι-CARs) from Solieria filiformis (Rhodophyta) by enzymatic extraction (EE), refined hot-water extraction (RHWE) and hot-water extraction (HWE), and test (EE, s.c.) their anti-inflammatory effects in the peritonitis model using the Lambda-carrageenan (700 µg cavity-1, i.p.) as an inflammatory stimuli in rats. The activated partial thromboplastin time (APTT) was also evaluated in ι-CARs fractions, obtained by ion-exchange chromatography (DEAE-cellulose), using rabbit plasma and compared to heparin (193 IU mg-1). The results showed that the ι-CARs (EE) (3 or 9 mg kg-1) containing 89.92% total sugars, 29.02% sulfate and absence of contaminant proteins inhibited (p < 0.05) the cellular infiltrate in the peritoneal cavity of the animals, but with 27 mg kg-1 had no anti-inflammatory effect (p > 0.05). Similar chromatography profiles were obtained among the methods; however, with fractions revealing different pattern on charge density by electrophoresis. Fractions had no virtually effects on APTT (1.16, 1.73 and 1.59 IU mg-1 for EE, RHWE and HWE, respectively). Further investigations to better understanding the actions of S. filiformis ι-CARs (EE) in the inflammatory response are suggested. |
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Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201red algasulfated galactansextraction methodsperitonitisAPTT testQuímica de MacromoléculasBiochemical analyses are important tools for discovering new bioactive compounds for medical clinic. This study aimed at isolating iota-carrageenans (ι-CARs) from Solieria filiformis (Rhodophyta) by enzymatic extraction (EE), refined hot-water extraction (RHWE) and hot-water extraction (HWE), and test (EE, s.c.) their anti-inflammatory effects in the peritonitis model using the Lambda-carrageenan (700 µg cavity-1, i.p.) as an inflammatory stimuli in rats. The activated partial thromboplastin time (APTT) was also evaluated in ι-CARs fractions, obtained by ion-exchange chromatography (DEAE-cellulose), using rabbit plasma and compared to heparin (193 IU mg-1). The results showed that the ι-CARs (EE) (3 or 9 mg kg-1) containing 89.92% total sugars, 29.02% sulfate and absence of contaminant proteins inhibited (p < 0.05) the cellular infiltrate in the peritoneal cavity of the animals, but with 27 mg kg-1 had no anti-inflammatory effect (p > 0.05). Similar chromatography profiles were obtained among the methods; however, with fractions revealing different pattern on charge density by electrophoresis. Fractions had no virtually effects on APTT (1.16, 1.73 and 1.59 IU mg-1 for EE, RHWE and HWE, respectively). Further investigations to better understanding the actions of S. filiformis ι-CARs (EE) in the inflammatory response are suggested.Universidade Estadual De Maringá2011-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionbioprodutoapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1120110.4025/actascitechnol.v34i2.11201Acta Scientiarum. Technology; Vol 34 No 2 (2012); 127-135Acta Scientiarum. Technology; v. 34 n. 2 (2012); 127-1351806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201/pdf_1Araújo, Ianna Wivianne Fernandes deRodrigues, José Ariévilo GurgelVanderlei, Edfranck de Sousa Oliveirade Paula, Gabriela AlmeidaLima, Ticiana de BritoBenevides, Norma Maria Barrosinfo:eu-repo/semantics/openAccess2024-05-17T13:03:18Zoai:periodicos.uem.br/ojs:article/11201Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:03:18Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201 |
title |
Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201 |
spellingShingle |
Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201 Araújo, Ianna Wivianne Fernandes de red alga sulfated galactans extraction methods peritonitis APTT test Química de Macromoléculas |
title_short |
Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201 |
title_full |
Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201 |
title_fullStr |
Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201 |
title_full_unstemmed |
Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201 |
title_sort |
Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation - doi: 10.4025/actascitechnol.v34i2.11201 |
author |
Araújo, Ianna Wivianne Fernandes de |
author_facet |
Araújo, Ianna Wivianne Fernandes de Rodrigues, José Ariévilo Gurgel Vanderlei, Edfranck de Sousa Oliveira de Paula, Gabriela Almeida Lima, Ticiana de Brito Benevides, Norma Maria Barros |
author_role |
author |
author2 |
Rodrigues, José Ariévilo Gurgel Vanderlei, Edfranck de Sousa Oliveira de Paula, Gabriela Almeida Lima, Ticiana de Brito Benevides, Norma Maria Barros |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Ianna Wivianne Fernandes de Rodrigues, José Ariévilo Gurgel Vanderlei, Edfranck de Sousa Oliveira de Paula, Gabriela Almeida Lima, Ticiana de Brito Benevides, Norma Maria Barros |
dc.subject.por.fl_str_mv |
red alga sulfated galactans extraction methods peritonitis APTT test Química de Macromoléculas |
topic |
red alga sulfated galactans extraction methods peritonitis APTT test Química de Macromoléculas |
description |
Biochemical analyses are important tools for discovering new bioactive compounds for medical clinic. This study aimed at isolating iota-carrageenans (ι-CARs) from Solieria filiformis (Rhodophyta) by enzymatic extraction (EE), refined hot-water extraction (RHWE) and hot-water extraction (HWE), and test (EE, s.c.) their anti-inflammatory effects in the peritonitis model using the Lambda-carrageenan (700 µg cavity-1, i.p.) as an inflammatory stimuli in rats. The activated partial thromboplastin time (APTT) was also evaluated in ι-CARs fractions, obtained by ion-exchange chromatography (DEAE-cellulose), using rabbit plasma and compared to heparin (193 IU mg-1). The results showed that the ι-CARs (EE) (3 or 9 mg kg-1) containing 89.92% total sugars, 29.02% sulfate and absence of contaminant proteins inhibited (p < 0.05) the cellular infiltrate in the peritoneal cavity of the animals, but with 27 mg kg-1 had no anti-inflammatory effect (p > 0.05). Similar chromatography profiles were obtained among the methods; however, with fractions revealing different pattern on charge density by electrophoresis. Fractions had no virtually effects on APTT (1.16, 1.73 and 1.59 IU mg-1 for EE, RHWE and HWE, respectively). Further investigations to better understanding the actions of S. filiformis ι-CARs (EE) in the inflammatory response are suggested. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-11-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion bioproduto |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201 10.4025/actascitechnol.v34i2.11201 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201 |
identifier_str_mv |
10.4025/actascitechnol.v34i2.11201 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/11201/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 34 No 2 (2012); 127-135 Acta Scientiarum. Technology; v. 34 n. 2 (2012); 127-135 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315334271860736 |