The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1792 |
Resumo: | Alginate is a polysaccharide extracted from cell walls of brown algae and used in the food, pharmaceuticals and biotech industries. Production is concentrated on the cultivation of brown seaweed, but several bacteria of the genus Pseudomonas and Azotobacter produce alginate. The chemical structure of alginates produced by algae is similar to those synthesized by A. vinelandii. The bacteria also produce intracellular polymers such as polyhydroxybutyrate (PHB), known as bioplastic. This work studied the simultaneous alginate and PHB production by A. vinelandii using sucrose and different parameters of fermentation in an orbital shaker. The optimal values for the production of these compounds were determined by the MSR. The first experiment was a 26-2 factorial design. The second was based on significant variables of the first, resulting in a full 33-0 factorial design. From the first to the second, an increase was observed in the PHB productivity from 12 to 45 mg g-1 cell h-1 and alginate from 100 to 1,600 mg g-1 of cell h-1. The productivity of both compounds was in the maximum incubation temperature of 62ºC, in the shortest time of incubation (18h) and the sucrose concentration, 11 g L-1. In both experiments the PHB extracted presented purity of 94%. |
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The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792Alginatepolyhydroxybutyrate (PHB)productionAzotobacter vinelandiiAlginatopoli-hidroxibutirato (PHB)produçãoAzotobacter vinelandiiCiência e Tecnologia de AlimentosAlginate is a polysaccharide extracted from cell walls of brown algae and used in the food, pharmaceuticals and biotech industries. Production is concentrated on the cultivation of brown seaweed, but several bacteria of the genus Pseudomonas and Azotobacter produce alginate. The chemical structure of alginates produced by algae is similar to those synthesized by A. vinelandii. The bacteria also produce intracellular polymers such as polyhydroxybutyrate (PHB), known as bioplastic. This work studied the simultaneous alginate and PHB production by A. vinelandii using sucrose and different parameters of fermentation in an orbital shaker. The optimal values for the production of these compounds were determined by the MSR. The first experiment was a 26-2 factorial design. The second was based on significant variables of the first, resulting in a full 33-0 factorial design. From the first to the second, an increase was observed in the PHB productivity from 12 to 45 mg g-1 cell h-1 and alginate from 100 to 1,600 mg g-1 of cell h-1. The productivity of both compounds was in the maximum incubation temperature of 62ºC, in the shortest time of incubation (18h) and the sucrose concentration, 11 g L-1. In both experiments the PHB extracted presented purity of 94%.O alginato é um polissacarídeo normalmente extraído de paredes celulares de algas marrons e utilizado nas indústrias de alimentos, farmacêuticas e biotecnológicas. A produção é concentrada no cultivo de algas marinhas marrons, mas várias bactérias do gênero Pseudomonas e Azotobacter produzem alginato. A estrutura química dos alginatos produzidos por algas é similar a dos sintetizados pela A. vinelandii. Esta bactéria também produz polímeros intracelulares como o poli-hidroxibutirato (PHB), conhecido como bioplástico. Neste trabalho, estudou-se a produção simultânea do alginato e PHB pela A. vinelandii, utilizando-se sacarose e diferentes parâmetros de fermentação em agitador orbital rotatório. Os valores ótimos para produção destes compostos foram determinados pela MSR. O 1º experimento foi um planejamento fatorial fracionado 26-2. O 2º foi baseado nas variáveis significativas do 1º experimento, resultando em um planejamento fatorial completo 33-0. Verificou-se, do primeiro para o segundo, aumento na produtividade do PHB de 12 para 45 mg g-1 de célula h-1 e do alginato de 100 para 1.600 mg g-1 de célula h-1. A produtividade de ambos os compostos foi máxima na temperatura de incubação de 62ºC, no menor tempo de incubação (18h) e na concentração de sacarose de 11 g L-1. Em ambos os experimentos, o PHB extraído apresentou pureza de 94%.Universidade Estadual De Maringá2010-07-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionprodução ; fermentação ; metodologia de superfície de respostaapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/179210.4025/actascitechnol.v32i2.1792Acta Scientiarum. Technology; Vol 32 No 2 (2010); 105-112Acta Scientiarum. Technology; v. 32 n. 2 (2010); 105-1121806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1792/1792Silva, Adriana Navarro daGarcia-Cruz, Crispin Humbertoinfo:eu-repo/semantics/openAccess2024-05-17T13:02:40Zoai:periodicos.uem.br/ojs:article/1792Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:02:40Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 |
title |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 |
spellingShingle |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 Silva, Adriana Navarro da Alginate polyhydroxybutyrate (PHB) production Azotobacter vinelandii Alginato poli-hidroxibutirato (PHB) produção Azotobacter vinelandii Ciência e Tecnologia de Alimentos |
title_short |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 |
title_full |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 |
title_fullStr |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 |
title_full_unstemmed |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 |
title_sort |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii - doi: 10.4025/actascitechnol.v32i2.1792 |
author |
Silva, Adriana Navarro da |
author_facet |
Silva, Adriana Navarro da Garcia-Cruz, Crispin Humberto |
author_role |
author |
author2 |
Garcia-Cruz, Crispin Humberto |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Silva, Adriana Navarro da Garcia-Cruz, Crispin Humberto |
dc.subject.por.fl_str_mv |
Alginate polyhydroxybutyrate (PHB) production Azotobacter vinelandii Alginato poli-hidroxibutirato (PHB) produção Azotobacter vinelandii Ciência e Tecnologia de Alimentos |
topic |
Alginate polyhydroxybutyrate (PHB) production Azotobacter vinelandii Alginato poli-hidroxibutirato (PHB) produção Azotobacter vinelandii Ciência e Tecnologia de Alimentos |
description |
Alginate is a polysaccharide extracted from cell walls of brown algae and used in the food, pharmaceuticals and biotech industries. Production is concentrated on the cultivation of brown seaweed, but several bacteria of the genus Pseudomonas and Azotobacter produce alginate. The chemical structure of alginates produced by algae is similar to those synthesized by A. vinelandii. The bacteria also produce intracellular polymers such as polyhydroxybutyrate (PHB), known as bioplastic. This work studied the simultaneous alginate and PHB production by A. vinelandii using sucrose and different parameters of fermentation in an orbital shaker. The optimal values for the production of these compounds were determined by the MSR. The first experiment was a 26-2 factorial design. The second was based on significant variables of the first, resulting in a full 33-0 factorial design. From the first to the second, an increase was observed in the PHB productivity from 12 to 45 mg g-1 cell h-1 and alginate from 100 to 1,600 mg g-1 of cell h-1. The productivity of both compounds was in the maximum incubation temperature of 62ºC, in the shortest time of incubation (18h) and the sucrose concentration, 11 g L-1. In both experiments the PHB extracted presented purity of 94%. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-07-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion produção ; fermentação ; metodologia de superfície de resposta |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1792 10.4025/actascitechnol.v32i2.1792 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1792 |
identifier_str_mv |
10.4025/actascitechnol.v32i2.1792 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/1792/1792 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 32 No 2 (2010); 105-112 Acta Scientiarum. Technology; v. 32 n. 2 (2010); 105-112 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
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1799315332033150976 |