Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPG |
Texto Completo: | http://tede2.uepg.br/jspui/handle/prefix/966 |
Resumo: | The Type 2 Diabetes Mellitus, as well as the metabolic syndrome (MS), is a multifactorial and metabolic disorder that now presents itself as a worldwide pandemic with effects on morbidity and mortality, possibly as a result of the mismatch between biological and cultural evolution of man. Was the object of research of this study to analyze the tissue expression of incretin hormones glucagon Pepitide Like-1 (GLP1) and Pepitide YY (PYY3-36), to identify and quantify L cells along the gastrointestinal tract in patients with DM2 subjected to adaptative gastroenteromentectomia with intestinal bipartition (Partial Duodenal Switch - DSP). The study was approved by the Ethics Committee of UEPG and patients informed and educated about the research objectives. The volunteer group consisted of 7 patients aged between 35 and 65 years, body mass index> 25 kg/m2 with T2DM on dietary treatment and medication for a minimum of 2 years and with difficulty on glycemic control and hypertriglyceridemia associated. Samples were obtained from the intestinal mucosa (jejunum and ileum) of DSP in patients undergoing preoperative and postoperative condition of fasting for 12 hours (three and twelve months respectively), through incisional biopsy. These biopsies were designed to test immunohistochemistry, qRT-PCR (Quantitative Real Time PCR) and Western Blott. The results were consistent and indicate a very significant differential expression between the state of pre-and postoperative tests for qRT-PCR (p = 0.1669) and Western Blot (p = 0.1569). Immunohistochemistry also showed low significance (p = 0.0043) of immune marked L cells for the same patients under the same conditions. These data can be interpreted in light of the fast imposed on patients. In addition the results are unprecedented for the immunostaining of L-cells of the human gastrointestinal tract, the data indicate that these cells have basal secretion for GLP-1, even after 12 hours without feed stimulation. In addition, the patients showed normalization of blood glucose levels in the post-surgery, suggesting metabolic improvement. It was also found that the number of L cells marked increases in density along the gastrointestinal tract toward the distal portion of the ileum (p = 0.0409). With these results it was possible to identify, locate and investigate different levels of expression and secretion from intestinal L cells in patients with DM2 and subjected to surgical control. |
id |
UEPG_641ec5e5735c09702b425273c9d43324 |
---|---|
oai_identifier_str |
oai:tede2.uepg.br:prefix/966 |
network_acronym_str |
UEPG |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UEPG |
repository_id_str |
|
spelling |
Artoni, Roberto FerreiraCPF:13854979800http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728593H6Campagnoli, Eduardo BaumlCPF:00407795941http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771395E4Milléo, Fábio QuirilhoCPF:30233712968http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4255179E1Faveró, Giovani MarinoCPF:02640460994http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4770965A4CPF:11605628735http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4242631Y6Estabile, Priscila Costa2017-07-21T19:59:56Z2014-02-212017-07-21T19:59:56Z2012-02-13ESTABILE, Priscila Costa. Evaluation of the incretin GLP-1 and PYY in patiens with Diabetes Mellitus 2 undergoing Partial Duodenal Swicth. 2012. 83 f. Dissertação (Mestrado em Biologia Evolutiva) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2012.http://tede2.uepg.br/jspui/handle/prefix/966The Type 2 Diabetes Mellitus, as well as the metabolic syndrome (MS), is a multifactorial and metabolic disorder that now presents itself as a worldwide pandemic with effects on morbidity and mortality, possibly as a result of the mismatch between biological and cultural evolution of man. Was the object of research of this study to analyze the tissue expression of incretin hormones glucagon Pepitide Like-1 (GLP1) and Pepitide YY (PYY3-36), to identify and quantify L cells along the gastrointestinal tract in patients with DM2 subjected to adaptative gastroenteromentectomia with intestinal bipartition (Partial Duodenal Switch - DSP). The study was approved by the Ethics Committee of UEPG and patients informed and educated about the research objectives. The volunteer group consisted of 7 patients aged between 35 and 65 years, body mass index> 25 kg/m2 with T2DM on dietary treatment and medication for a minimum of 2 years and with difficulty on glycemic control and hypertriglyceridemia associated. Samples were obtained from the intestinal mucosa (jejunum and ileum) of DSP in patients undergoing preoperative and postoperative condition of fasting for 12 hours (three and twelve months respectively), through incisional biopsy. These biopsies were designed to test immunohistochemistry, qRT-PCR (Quantitative Real Time PCR) and Western Blott. The results were consistent and indicate a very significant differential expression between the state of pre-and postoperative tests for qRT-PCR (p = 0.1669) and Western Blot (p = 0.1569). Immunohistochemistry also showed low significance (p = 0.0043) of immune marked L cells for the same patients under the same conditions. These data can be interpreted in light of the fast imposed on patients. In addition the results are unprecedented for the immunostaining of L-cells of the human gastrointestinal tract, the data indicate that these cells have basal secretion for GLP-1, even after 12 hours without feed stimulation. In addition, the patients showed normalization of blood glucose levels in the post-surgery, suggesting metabolic improvement. It was also found that the number of L cells marked increases in density along the gastrointestinal tract toward the distal portion of the ileum (p = 0.0409). With these results it was possible to identify, locate and investigate different levels of expression and secretion from intestinal L cells in patients with DM2 and subjected to surgical control.Diabetes mellitus do tipo 2 (DM2), assim como a Síndrome Metabólica (SM), é uma desordem metabólica e multifatorial que atualmente se apresenta como pandemia mundial com reflexos na morbimortalidade, possivelmente em decorrência do descompasso entre a evolução biológica e cultural do homem. Foi objeto de investigação do presente estudo analisar a expressão tecidual dos hormônios incretínicos Glucagon Like Pepitide-1 (GLP1) e Pepitide YY (PYY3-36), visando identificar e quantificar células L ao longo do trato gastrointestinal, de pacientes portadores de DM2 submetidos a gastroenteromentectomia adaptativa com bipartição intestinal (Duodenal Switch Parcial - DSP). O trabalho foi aprovado pelo Comitê de Ética da UEPG e os pacientes informados e esclarecidos sobre os objetivos da pesquisa. O grupo de voluntários foi composto de 7 pacientes com idade entre 35 e 65 anos, Índice de Massa Corporal > 25 Kg/m2, com DM2 em tratamento dietético e medicamentoso por um período mínimo de 2 anos e com dificuldade de controle glicêmico e hipertrigliceridemia associada. Foram obtidas amostras da mucosa intestinal (jejuno e íleo) dos pacientes submetidos à DSP no pré-operatório e no pós-operatório em condição de jejum de 12 horas (três e doze meses, respectivamente), através de biópsia incisional. Estas biópsias foram destinadas aos ensaios de Imunohistoquímica, qRT-PCR (Quantitative Real Time PCR) e Western Blott. Os resultados obtidos foram congruentes e apontam uma expressão diferencial pouco significativa entre o estado de pré- e pós-operatório para os ensaios de qRT-PCR (p=0,1669) e Western Blott (p=0,1569). A imunohistoquímica mostrou também baixa significância (p=0,0043) de células L imuno marcadas para os mesmos pacientes, nas mesmas condições. Estes dados podem ser interpretados a luz do jejum imposto aos pacientes. Além dos resultados serem inéditos para a imunomarcação de células L do trato gastrointestinal humano, os dados obtidos indicam que estas células apresentam secreção basal para GLP-1, mesmo após 12 horas sem estímulo alimentar. Em adição, os pacientes apresentaram normalização dos níveis de glicemia no estado pós-operado, sugerindo melhora metabólica. Foi ainda constatado que o número de células L marcadas aumenta em densidade ao longo do trato gastrointestinal em direção à porção mais distal do íleo (p=0,0409). Com estes resultados foi possível identificar, localizar e investigar diferentes níveis de expressão e secreção das células L intestinais em pacientes portadores de DM2 e submetidos a controle cirúrgico.Made available in DSpace on 2017-07-21T19:59:56Z (GMT). No. of bitstreams: 1 Priscila Costa Estabile.pdf: 1735178 bytes, checksum: b258b31793d5d5954531a50cbe4206cc (MD5) Previous issue date: 2012-02-13Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paranáapplication/pdfporUNIVERSIDADE ESTADUAL DE PONTA GROSSAPrograma de Pós-Graduação em Ciências BiológicasUEPGBRBiologia EvolutivaDiabetes tipo 2cirurgia bariátricacélulas LGLP-1PYY3-36Type 2 diabetesbariatric surgerycells LGLP-1PYY3-36CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULARAvaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth ParcialEvaluation of the incretin GLP-1 and PYY in patiens with Diabetes Mellitus 2 undergoing Partial Duodenal Swicthinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPGinstname:Universidade Estadual de Ponta Grossa (UEPG)instacron:UEPGORIGINALPriscila Costa Estabile.pdfapplication/pdf1735178http://tede2.uepg.br/jspui/bitstream/prefix/966/1/Priscila%20Costa%20Estabile.pdfb258b31793d5d5954531a50cbe4206ccMD51prefix/9662017-07-21 16:59:56.496oai:tede2.uepg.br:prefix/966Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.uepg.br/jspui/PUBhttp://tede2.uepg.br/oai/requestbicen@uepg.br||mv_fidelis@yahoo.com.bropendoar:2017-07-21T19:59:56Biblioteca Digital de Teses e Dissertações da UEPG - Universidade Estadual de Ponta Grossa (UEPG)false |
dc.title.por.fl_str_mv |
Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial |
dc.title.alternative.eng.fl_str_mv |
Evaluation of the incretin GLP-1 and PYY in patiens with Diabetes Mellitus 2 undergoing Partial Duodenal Swicth |
title |
Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial |
spellingShingle |
Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial Estabile, Priscila Costa Diabetes tipo 2 cirurgia bariátrica células L GLP-1 PYY3-36 Type 2 diabetes bariatric surgery cells L GLP-1 PYY3-36 CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
title_short |
Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial |
title_full |
Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial |
title_fullStr |
Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial |
title_full_unstemmed |
Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial |
title_sort |
Avaliação das incretinas GLP-1 e PYY em pacientes com Diabetes Mellitus 2 submetidos a Duodenal Swicth Parcial |
author |
Estabile, Priscila Costa |
author_facet |
Estabile, Priscila Costa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Artoni, Roberto Ferreira |
dc.contributor.advisor1ID.fl_str_mv |
CPF:13854979800 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728593H6 |
dc.contributor.advisor-co1.fl_str_mv |
Campagnoli, Eduardo Bauml |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:00407795941 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771395E4 |
dc.contributor.referee1.fl_str_mv |
Milléo, Fábio Quirilho |
dc.contributor.referee1ID.fl_str_mv |
CPF:30233712968 |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4255179E1 |
dc.contributor.referee2.fl_str_mv |
Faveró, Giovani Marino |
dc.contributor.referee2ID.fl_str_mv |
CPF:02640460994 |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4770965A4 |
dc.contributor.authorID.fl_str_mv |
CPF:11605628735 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4242631Y6 |
dc.contributor.author.fl_str_mv |
Estabile, Priscila Costa |
contributor_str_mv |
Artoni, Roberto Ferreira Campagnoli, Eduardo Bauml Milléo, Fábio Quirilho Faveró, Giovani Marino |
dc.subject.por.fl_str_mv |
Diabetes tipo 2 cirurgia bariátrica células L GLP-1 PYY3-36 |
topic |
Diabetes tipo 2 cirurgia bariátrica células L GLP-1 PYY3-36 Type 2 diabetes bariatric surgery cells L GLP-1 PYY3-36 CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
dc.subject.eng.fl_str_mv |
Type 2 diabetes bariatric surgery cells L GLP-1 PYY3-36 |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
description |
The Type 2 Diabetes Mellitus, as well as the metabolic syndrome (MS), is a multifactorial and metabolic disorder that now presents itself as a worldwide pandemic with effects on morbidity and mortality, possibly as a result of the mismatch between biological and cultural evolution of man. Was the object of research of this study to analyze the tissue expression of incretin hormones glucagon Pepitide Like-1 (GLP1) and Pepitide YY (PYY3-36), to identify and quantify L cells along the gastrointestinal tract in patients with DM2 subjected to adaptative gastroenteromentectomia with intestinal bipartition (Partial Duodenal Switch - DSP). The study was approved by the Ethics Committee of UEPG and patients informed and educated about the research objectives. The volunteer group consisted of 7 patients aged between 35 and 65 years, body mass index> 25 kg/m2 with T2DM on dietary treatment and medication for a minimum of 2 years and with difficulty on glycemic control and hypertriglyceridemia associated. Samples were obtained from the intestinal mucosa (jejunum and ileum) of DSP in patients undergoing preoperative and postoperative condition of fasting for 12 hours (three and twelve months respectively), through incisional biopsy. These biopsies were designed to test immunohistochemistry, qRT-PCR (Quantitative Real Time PCR) and Western Blott. The results were consistent and indicate a very significant differential expression between the state of pre-and postoperative tests for qRT-PCR (p = 0.1669) and Western Blot (p = 0.1569). Immunohistochemistry also showed low significance (p = 0.0043) of immune marked L cells for the same patients under the same conditions. These data can be interpreted in light of the fast imposed on patients. In addition the results are unprecedented for the immunostaining of L-cells of the human gastrointestinal tract, the data indicate that these cells have basal secretion for GLP-1, even after 12 hours without feed stimulation. In addition, the patients showed normalization of blood glucose levels in the post-surgery, suggesting metabolic improvement. It was also found that the number of L cells marked increases in density along the gastrointestinal tract toward the distal portion of the ileum (p = 0.0409). With these results it was possible to identify, locate and investigate different levels of expression and secretion from intestinal L cells in patients with DM2 and subjected to surgical control. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-13 |
dc.date.available.fl_str_mv |
2014-02-21 2017-07-21T19:59:56Z |
dc.date.accessioned.fl_str_mv |
2017-07-21T19:59:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ESTABILE, Priscila Costa. Evaluation of the incretin GLP-1 and PYY in patiens with Diabetes Mellitus 2 undergoing Partial Duodenal Swicth. 2012. 83 f. Dissertação (Mestrado em Biologia Evolutiva) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2012. |
dc.identifier.uri.fl_str_mv |
http://tede2.uepg.br/jspui/handle/prefix/966 |
identifier_str_mv |
ESTABILE, Priscila Costa. Evaluation of the incretin GLP-1 and PYY in patiens with Diabetes Mellitus 2 undergoing Partial Duodenal Swicth. 2012. 83 f. Dissertação (Mestrado em Biologia Evolutiva) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2012. |
url |
http://tede2.uepg.br/jspui/handle/prefix/966 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UNIVERSIDADE ESTADUAL DE PONTA GROSSA |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas |
dc.publisher.initials.fl_str_mv |
UEPG |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biologia Evolutiva |
publisher.none.fl_str_mv |
UNIVERSIDADE ESTADUAL DE PONTA GROSSA |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UEPG instname:Universidade Estadual de Ponta Grossa (UEPG) instacron:UEPG |
instname_str |
Universidade Estadual de Ponta Grossa (UEPG) |
instacron_str |
UEPG |
institution |
UEPG |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UEPG |
collection |
Biblioteca Digital de Teses e Dissertações da UEPG |
bitstream.url.fl_str_mv |
http://tede2.uepg.br/jspui/bitstream/prefix/966/1/Priscila%20Costa%20Estabile.pdf |
bitstream.checksum.fl_str_mv |
b258b31793d5d5954531a50cbe4206cc |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UEPG - Universidade Estadual de Ponta Grossa (UEPG) |
repository.mail.fl_str_mv |
bicen@uepg.br||mv_fidelis@yahoo.com.br |
_version_ |
1809460452096212992 |