Avaliação da atividade antineoplásica e anti inflamatória do óleo essencial e do extrato etanólico de Myrciaria glomerata O. Berg

• Autor(a) principal: Silva, Nemes Veiga Pacheco
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/7918
• topic: Cancer; Cell cycle; Cell Migration; Myrtaceae; Medicinal plants; Câncer; Ciclo celular; Migração celular; Myrtaceae; Plantas medicinais; Plantas medicinais Uso terapêutico; Óleos essenciais; CNPQ::CIENCIAS BIOLOGICAS::BOTANICA

Myrciaria glomerata O. Berg, popularly known as hairy hair , has ascorbic acid and proven antimicrobial and antifungal properties. The objectives of this work involved the in vitro study of antineoplastic and antiinflammatory activity of Essential Oil (EO) and Ethanolic Extract (EE), extracted from M. glomerata leaves, collected in Tijuca Forest in Rio de Janeiro. The extraction of the EO was performed from the fresh biomass conditioned in Clevenger type apparatus, while the EE was obtained by exhaustive maceration with ethanol, filtration and evaporation of the solvent in rotary evaporator under reduced pressure, with yields of 0,17% (OE) e 7,67% (EE). The phytochemical study of M. glomerata materials was performed by High Performance Liquid Chromatography (HPLC) and Mass Spectrometer Gas Chromatography (GC-MS). The in vitro antineoplastic activity of OE and EE was investigated by MTT and trypan blue exclusion assays on leukemic (Jurkat and K562) and tumor (MCF-7, PC3 and A549) and VERO healthy cell lines. Its effects on cell cycle and apoptosis were studied with the Jurkat cells, which proved to be the most sensitive one. Antiinflammatory action was evaluated on macrophage lineage RAW 264.7 by nitric oxide (NO) production by Griess assay and cell migration by Wound healing and Boyding methods in LPS-activated RAW 264.7 macrophages in vitro. The EO, at concentrations of 50 and 100 μg / ml, reduced, respectively, the viability of K562 (11.24% and 11.77%), PC3 (15.65% and 25.21%), MCF7 (13). % and 27.8%) and Jurkat (14.84% and 28.31%). The EE (50 and 100 μg / ml) reduced, respectively, the viability of the K562 (11.42% and 15.74%), PC3 (21% and 21%), MCF7 (26.7% and 34.5) strains. %), Jurkat (16,5% and 77,6%) and A549 (20.0%) only at 100 μg / ml. The results obtained with M. glomerata showed for the first time that the extracted products had antineoplastic effect, mainly by EE on Jurkat cells (IC50 = 68.34 μg / ml), both cytostatic and cytotoxic, observed by the reduction of proliferation in a dose dependent manner and by increasing apoptosis at concentrations of 100 and 200 μg / ml respectively, without presenting cytotoxicity to the VERO cells. Both M. glomerata products also had anti-inflammatory effect, by reducing the production of pro-inflammatory NO mediator at concentrations of 50 and 100 μg / ml for both OE (36.94% and 51.06%), as for EE (71.14% and 84.03%) respectively. In addition, both products inhibited cell migration of RAW 264.7 macrophages at both concentrations in the in vitro tested assays. In general, EE has been shown to have a higher biological effect than EO. The phytochemical analyzes of the EO identified 21 components, being the sesquiterpenes β-Elemeno (15.31%) and β-Cariophilene (13.22%) the major constituents. EE phytochemistry suggests the presence of flavonoids. Thus, M. glomerata has shown to be a promising potential herbal remedy for the treatment of these high mortality diseases in the population.