Efeitos do extrato de Chenopodium ambrosioides L. na cistite induzida pela ciclofosfamida

• Autor(a) principal: Gonçalves Filho, Antonio
• Data de Publicação: 2013
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/12630
• topic: Chenopodium ambrosioides; Cystitis; Cyclophosphamide; Anti-inflammatory; Chenopodium ambrosioides; Cistite; Ciclofosfamida; Anti-inflamatório; Produtos naturais; Agentes antiinflamatórios; CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA

Hemorrhagic cystitis (HC) consists of a diffuse inflammatory process that results on the bleeding of the bladder mucosa due to infectious or noninfectious etiology. Chronically recurrent CYP induced HC remains a challenge to clinical practice given its high morbidity and sometimes mortality of patients. Treatment consists of administering MESNA, disulfiram, N-acetylcysteine, anti-inflammatory, hyperbaric oxygen, hyperhydration and bladder irrigation, though new therapies have been investigated, such as the use of natural products. Popularly known as mastruz, mastruço and erva-de-Santa-Maria, Chenopodium ambrosioides L. has been generally reported by people as having anti-inflammatory and analgesic effects. The following study investigated the effects of a hydroalcoholic crude extract (EBH) of Chenopodium ambrosioides leaves on cyclophosphamide (CYP) induced HC in rats. In order to induce HC, twenty nine rats were intraperitoneally (i.p.) administered 150mg/kg of CYP and then divided into three groups: negative control (NC) were treated with 0,9% saline solution; hydroalcoholic crude extract of Chenopodium ambrosioides (EBHCa) were treated with a single dose of 50 mg/kg of EBH administered and positive control (PC) were treated with a single dose of 15 mg/kg of diclofenac potassium, all of them by gavage. After 48 hours of HC induction, all rats were sacrificed, their bladders removed and prepared for histopathological and immunohistochemical analysis. EBH was able to decrease bladder weight and histologically decrease acute and chronic bladder inflammation, decrease on the infiltrated inflammatory extension of the bladder wall and capillary neoformation in the same way of those from group CN. There was also a reduction in the immunohistochemical expression of cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NFB) in the bladder. In this study, Chenopodium ambrosioides leaves EBH showed anti-inflammatory activity which was similar to results from diclofenac potassium used on treatment of CYP induced HC.