Análise comparativa do metaboloma salivar em pacientes com periodontite e doença inflamatória intestinal

• Autor(a) principal: Curcio, Heloisa Fernandes Queiroz
• Data de Publicação: 2022
• Outros Autores: [e-mail]
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/19082
• topic: Periodontitis; Inflammatory bowel disease; Metabolome; Periodontite; Doença inflamatória intestinal; Metaboloma; CIENCIAS DA SAUDE::ODONTOLOGIA::PERIODONTIA

Inflammatory Bowel Disease (IBD) and periodontitis are characterized by a complex etiopathogenesis, late diagnosis and for causing a worsening in the quality of life of individuals, so the identification of reliable biomarkers for an early and accurate diagnosis is an essential point for the correct management of these diseases. conditions. The aim of this study was to evaluate salivary metabolome in IBD patients with periodontitis and healthy patients without periodontitis. For each of the study participants, a medical record containing personal data, anamnesis, data related to the gastrointestinal system and dental data (visible plaque index, and periogram composed of pocket depth on probing, clinical attachment loss and bleeding on probing) was filled out. . Forty-nine patients were selected, divided into four groups, seven with Crohn's Disease (CD) and periodontitis, five with Idiopathic Ulcerative Colitis (IUGR) and periodontitis, twenty-three systemically healthy patients with periodontitis and fourteen systemically healthy patients without periodontitis. The samples consisted of 4mL of unstimulated saliva. For each patient, 1mL of saliva was added to 20μl of protease inhibitor, centrifuged for 8 minutes at 8000 revolutions per minute and at laboratory temperature. Then, the saliva supernatant was transferred to a new Eppendorf and frozen at -80°C until the samples were processed. Afterwards, the samples were submitted to analysis in a 500 MHz Bruker nuclear magnetic resonance spectrometer, the spectra obtained were submitted to multivariate analysis using PLS-DA, sPLS-DA and univariate analysis using chi-square and t tests (SPSS 20.0, IL, USA), with a significance level of p < 0.05. Thirteen metabolites were identified in the saliva samples (butyrate, caproate, ethanol, glucose, sucrose, glutamine, glutamate, hydroxybutyrate, threonine, lysine, valine and N-acetyl group). Patients with CD and periodontitis had statistically significant levels of butyrate, caproate, ethanol and hydroxybutyrate when associated with controls with periodontitis. Between patients with UC and periodontitis and controls with periodontitis, there was a statistically significant difference in the levels of butyrate, caproate, sugar region, threonine and valine. Among controls with and without periodontitis, isoleucine levels were statistically significant. The analysis of saliva by NMR was able to reveal significant differences in the metabolic profile between the four groups analyzed, showing that there may be a relationship, at the metabolic level, between IBD and periodontitis. However, more studies are needed and it is important that there is methodological standardization in future studies.