Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica

• Autor(a) principal: Silva, Fernanda de Brito
• Data de Publicação: 2008
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14036
• topic: Periodontitis; Inflammatory bowel disease; Cytokines; Th cell profile; Intestinos - Doenças inflamatórias; Crohn, Doença de; Colite ulcerativa; Citocinese; Células T; Periodontite; Doença inflamatória intestinal; Citocinas; Perfil linfocitário; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::PERIODONTIA

The aim of this thesis was to evaluate the expression of Th1 cytokines (IL-12 and INF-γ), Th2 cytokines (IL-4, IL-6 and IL-10) and the pro-inflammatory cytokines IL-18, IL-1 and TNF-α in the gingival crevicular fluid (GCF) from Crohn s disease (CD) patients, ulcerative colitis (UC) patients and healthy individuals (control group, CG) who had chronic periodontitis. Besides, we measured elastase activity, matrix metalloproteinase -8 and -9 (MMP-8 and -9) to address the neutrophil function in the GCF. Fifteen CD patients (mean age 38.2  11.4 years), 15 UC patients (mean age 45.0  10.5 years) and 15 systemically healthy controls (mean age 42.1  7.8 years) were enrolled in this study. All the present teeth, except for the third molars were examined. Probing pocket depth (PPD), clinical attachment loss (CAL), presence of plaque and presence of bleeding on probing were assessed in six sites per tooth. In every subject, GCF from 4 gingivitis sites (PPD  3mm and CAL  1mm) and from 4 periodontitis sites (PPD  5mm and CAL 3mm) were collected with filter strips. The data were reported as total amount and concentration. IL-1, IL-4, IL-6, IL-10, IL-12p70, TNFα, INFγ, MMP-8 and MMP-9 were analyzed by the Luminex® analyzer. IL-18 was analyzed using a commercially available ELISA assay and the elastase activity by an enzymatic reaction. The serum was also analysed and the correlations between the cytokines in the GCF and in the serum were calculated by Pearson correlation analysis. In gingivitis sites, the total amount of IL-4 was significantly lower in the UC group than in the CG group (p=0.016). In periodontitis sites, the total amount of IL-4 was significantly lower in CD group than in the CG group (p=0.029). The total amount of IL-4 was lower in UC group than in CD group (p=0.077). Similarly, IL-4 concentrations in both CD (p=0.096) and UC (p=0.064) groups were lower than in CG group. IL-6 total amount (p=0.028) and IL-6 concentration (p=0.044) were significantly higher in the UC group than in the CG group. In the serum, IL-18 levels were significantly higher in CD (p=0.011) and UC (p=0.019) groups than in the CG group. In UC group, there was a positive correlation between serum IL-18 levels and IL-1 in the GCF (r=0. 667, p=0.01). In conclusion, IBD patients had lower IL-4 levels in the GCF and higher IL-18 levels in the serum than healthy controls. The neutrophil function in IBD patients is similar to controls. Except for the positive correlation between IL-18 in the serum and IL-1 in the GCF, there was no correlation between the cytokines in serum and in the GCF either in IBD patients or in controls. There was not a Th1 or Th2 polarization in the GCF from IBD patients.