Análise da influência de proteínas da matriz extracelular e fibrinogênio na formação de biofilme por cepas de Enterococcus faecalis isoladas de infecção endodôntica primária

• Autor(a) principal: Soares, Yanna de Omena
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14196
• topic: Biofilms; Periapical periodontitis; Extracelular matrix; Fibronectin receptors; Collagen receptors; Fibrinogen; Enterococcus faecalis; Biofilmes; Periodontite apical; Matriz extracelular; Receptores de fibronectina; Receptores de colágeno; Fibrinogênio; Enterococcus faecalis; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::ENDODONTIA

Enterococcus faecalis is an opportunistic pathogen with peculiar potential to maintain the periradicular endodontic infection even after chemical-mechanical preparation of the root canal system. In addition, it has the ability to develop into biofilms and presents in your cell wall adhesins compatible with collagenous substrates, as the composition of the extracellular matrix of the dentine and dentinal tubules. This study aims to characterize genetically 23 samples of E. faecalis isolated from primary endodontic infections by Polymerase Chain Reaction (PCR) technique and investigate the influence of collagen type I (COL I), fibronectin (FN) and fibrinogen (FBG) in biofilm formation on abiotic surface. Thus, after the sensitization of ¾ the wells of sterile microtiter plates with 50 ul of matrix protein solution (COL I and FN FBG) at a concentration of 1mg / ml, was transferred 50mL of bacterial suspension (1.5 x 108 bact / ml) corresponding to each sample in order to fill both wells sensitized and non-sensitized. Quantification of biofilm formation was performed by optical density, so the results showed that there were biofilm formation by all strains on abiotic surface, but with different degrees of intensity. All strains were genetically identified as Enterococcus faecalis and the presence of gelE gene was prevalent. However, none showed amplification for the esp and agg gene, and, while 73.9% of the samples for amplifying ace gene, only 2 strains (P7 and P75) isolated from primary endodontic infections they had increased biofilm formation in the presence of COL I (P <0.05). Although the presence of FBG no provides significant support for the biofilm formation, COL I and FN were relevant influence in the reduction of biofilm formation for most of the samples. It is possible that the biofilm-forming ability inherent in E. faecalis and affinity for FN and COL I through ace gene expression contribute substantially to maintain of this microorganism in the root environment even after thorough endodontic treatment.