Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFAM |
Texto Completo: | http://riu.ufam.edu.br/handle/prefix/6924 |
Resumo: | Resistance to antimicrobial drugs has become a serious public health problem due to indiscriminate use by humans. Therefore, several alternatives have been studied to contain super microorganisms that cause diseases, among them the use of secondary compounds produced by plants together with endophytic microorganisms. Therefore, this work aimed to isolate, identify and analyze the antimicrobial potential of endophytic microorganisms of Piper hispidinervum C. DC. against pathogens that affect humans and different animals. First, the leaf and inflorescence were collected and the plant material was identified. The material collected went through the process of asepsis and inoculation, which resulted in the growth of endophytes. After their growth, several replications were performed to purify the colonies. Regarding the macromorphology of endophytic fungi, different textures and colors were observed. Were obtained 27 isolates of fungal cultures, among them the most found genera are Colletotrichum sp., and Fusarium sp., we also identified a species of Curvularia sp. The rest of the isolates were classified as Mycelia sterilia. For the antimicrobial susceptibility tests, the inoculum of the test pathogens was carried out in Mueller- Hinton culture medium and the suspension was adjusted to the MacFarland scale. To obtain the fungal extract, colony fragments were deposited in Erlenmeyer flasks with Potato-Dextrose medium, after 14 days the culture medium was filtered, then Ethyl Acetate was added to extract metabolites. The fungal extract obtained by Ethyl Acetate was used in the sensitivity test by the well diffusion method. The Fusarium Extract sp. esp07 was the most effective against the microorganisms tested, being able to inhibit S. aureus, E. coli and C. albicans, resulting in halos with an average of 23 mm, 21 mm and 14 mm, respectively. The Fusarium sp. esp04 and Curvularia sp. fol50 have also been shown to be effective against E. coli. |
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Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervumPiper sp.Fungal extractsAntimicrobial potentialCIENCIAS BIOLOGICASAntibacterianosAnti-InfecciososPiperResistance to antimicrobial drugs has become a serious public health problem due to indiscriminate use by humans. Therefore, several alternatives have been studied to contain super microorganisms that cause diseases, among them the use of secondary compounds produced by plants together with endophytic microorganisms. Therefore, this work aimed to isolate, identify and analyze the antimicrobial potential of endophytic microorganisms of Piper hispidinervum C. DC. against pathogens that affect humans and different animals. First, the leaf and inflorescence were collected and the plant material was identified. The material collected went through the process of asepsis and inoculation, which resulted in the growth of endophytes. After their growth, several replications were performed to purify the colonies. Regarding the macromorphology of endophytic fungi, different textures and colors were observed. Were obtained 27 isolates of fungal cultures, among them the most found genera are Colletotrichum sp., and Fusarium sp., we also identified a species of Curvularia sp. The rest of the isolates were classified as Mycelia sterilia. For the antimicrobial susceptibility tests, the inoculum of the test pathogens was carried out in Mueller- Hinton culture medium and the suspension was adjusted to the MacFarland scale. To obtain the fungal extract, colony fragments were deposited in Erlenmeyer flasks with Potato-Dextrose medium, after 14 days the culture medium was filtered, then Ethyl Acetate was added to extract metabolites. The fungal extract obtained by Ethyl Acetate was used in the sensitivity test by the well diffusion method. The Fusarium Extract sp. esp07 was the most effective against the microorganisms tested, being able to inhibit S. aureus, E. coli and C. albicans, resulting in halos with an average of 23 mm, 21 mm and 14 mm, respectively. The Fusarium sp. esp04 and Curvularia sp. fol50 have also been shown to be effective against E. coli.2NãoBrasilPonta Grossa2023-09-22T20:53:06Z2023-09-22T20:53:06Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2764-0159http://riu.ufam.edu.br/handle/prefix/6924engInternational Journal of Health ScienceAn error occurred getting the license - uri.info:eu-repo/semantics/openAccessAlbuquerque, Isabela Ribeiro deFreitas, Adriana Dantas Gonzaga deNobre, Janaina da Costa NogueiraSousa, Francy Mary GalúcioLuciano, Jackeline da SilvaFarias, Mariana NepomucenoSousa, Maria Lucidalva Ribeiro deCosta, Gerodes Vasconcelos daMonteiro, Antonio Geilson Matiasreponame:Repositório Institucional da UFAMinstname:Universidade Federal do Amazonas (UFAM)instacron:UFAM2023-09-22T20:56:07Zoai:localhost:prefix/6924Repositório InstitucionalPUBhttp://riu.ufam.edu.br/oai/requestopendoar:2023-09-22T20:56:07Repositório Institucional da UFAM - Universidade Federal do Amazonas (UFAM)false |
dc.title.none.fl_str_mv |
Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum |
title |
Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum |
spellingShingle |
Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum Albuquerque, Isabela Ribeiro de Piper sp. Fungal extracts Antimicrobial potential CIENCIAS BIOLOGICAS Antibacterianos Anti-Infecciosos Piper |
title_short |
Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum |
title_full |
Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum |
title_fullStr |
Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum |
title_full_unstemmed |
Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum |
title_sort |
Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum |
author |
Albuquerque, Isabela Ribeiro de |
author_facet |
Albuquerque, Isabela Ribeiro de Freitas, Adriana Dantas Gonzaga de Nobre, Janaina da Costa Nogueira Sousa, Francy Mary Galúcio Luciano, Jackeline da Silva Farias, Mariana Nepomuceno Sousa, Maria Lucidalva Ribeiro de Costa, Gerodes Vasconcelos da Monteiro, Antonio Geilson Matias |
author_role |
author |
author2 |
Freitas, Adriana Dantas Gonzaga de Nobre, Janaina da Costa Nogueira Sousa, Francy Mary Galúcio Luciano, Jackeline da Silva Farias, Mariana Nepomuceno Sousa, Maria Lucidalva Ribeiro de Costa, Gerodes Vasconcelos da Monteiro, Antonio Geilson Matias |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Albuquerque, Isabela Ribeiro de Freitas, Adriana Dantas Gonzaga de Nobre, Janaina da Costa Nogueira Sousa, Francy Mary Galúcio Luciano, Jackeline da Silva Farias, Mariana Nepomuceno Sousa, Maria Lucidalva Ribeiro de Costa, Gerodes Vasconcelos da Monteiro, Antonio Geilson Matias |
dc.subject.por.fl_str_mv |
Piper sp. Fungal extracts Antimicrobial potential CIENCIAS BIOLOGICAS Antibacterianos Anti-Infecciosos Piper |
topic |
Piper sp. Fungal extracts Antimicrobial potential CIENCIAS BIOLOGICAS Antibacterianos Anti-Infecciosos Piper |
description |
Resistance to antimicrobial drugs has become a serious public health problem due to indiscriminate use by humans. Therefore, several alternatives have been studied to contain super microorganisms that cause diseases, among them the use of secondary compounds produced by plants together with endophytic microorganisms. Therefore, this work aimed to isolate, identify and analyze the antimicrobial potential of endophytic microorganisms of Piper hispidinervum C. DC. against pathogens that affect humans and different animals. First, the leaf and inflorescence were collected and the plant material was identified. The material collected went through the process of asepsis and inoculation, which resulted in the growth of endophytes. After their growth, several replications were performed to purify the colonies. Regarding the macromorphology of endophytic fungi, different textures and colors were observed. Were obtained 27 isolates of fungal cultures, among them the most found genera are Colletotrichum sp., and Fusarium sp., we also identified a species of Curvularia sp. The rest of the isolates were classified as Mycelia sterilia. For the antimicrobial susceptibility tests, the inoculum of the test pathogens was carried out in Mueller- Hinton culture medium and the suspension was adjusted to the MacFarland scale. To obtain the fungal extract, colony fragments were deposited in Erlenmeyer flasks with Potato-Dextrose medium, after 14 days the culture medium was filtered, then Ethyl Acetate was added to extract metabolites. The fungal extract obtained by Ethyl Acetate was used in the sensitivity test by the well diffusion method. The Fusarium Extract sp. esp07 was the most effective against the microorganisms tested, being able to inhibit S. aureus, E. coli and C. albicans, resulting in halos with an average of 23 mm, 21 mm and 14 mm, respectively. The Fusarium sp. esp04 and Curvularia sp. fol50 have also been shown to be effective against E. coli. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2023-09-22T20:53:06Z 2023-09-22T20:53:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
2764-0159 http://riu.ufam.edu.br/handle/prefix/6924 |
identifier_str_mv |
2764-0159 |
url |
http://riu.ufam.edu.br/handle/prefix/6924 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Health Science |
dc.rights.driver.fl_str_mv |
An error occurred getting the license - uri. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
An error occurred getting the license - uri. |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Brasil Ponta Grossa |
publisher.none.fl_str_mv |
Brasil Ponta Grossa |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFAM instname:Universidade Federal do Amazonas (UFAM) instacron:UFAM |
instname_str |
Universidade Federal do Amazonas (UFAM) |
instacron_str |
UFAM |
institution |
UFAM |
reponame_str |
Repositório Institucional da UFAM |
collection |
Repositório Institucional da UFAM |
repository.name.fl_str_mv |
Repositório Institucional da UFAM - Universidade Federal do Amazonas (UFAM) |
repository.mail.fl_str_mv |
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1798061064031567872 |