Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/13679 |
Resumo: | Texto completo: acesso restrito. p. 237–243 |
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Brites, CarlosSwanson, PriscilaHuang, ShihaiHolzmayer, VeraBodelle, PierreYamaguchi, JulieBrennan, Catherine A.Badaró, Roberto José da SilvaAbravaya, KlaraDevare, Sushil G.Hackett Junior, JohnBrites, CarlosSwanson, PriscilaHuang, ShihaiHolzmayer, VeraBodelle, PierreYamaguchi, JulieBrennan, Catherine A.Badaró, Roberto José da SilvaAbravaya, KlaraDevare, Sushil G.Hackett Junior, John2013-11-16T13:11:51Z20060166-0934http://repositorio.ufba.br/ri/handle/ri/13679v.134, n. 1-2Texto completo: acesso restrito. p. 237–243The combination of automated sample preparation and real-time RT-PCR for measurement of HIV-1 viral load has the potential to significantly enhance throughput, reduce operator-associated error, and increase assay sensitivity and dynamic range. In this study, RNA was extracted from the plasma of 91 HIV-1 seropositive Brazilian blood donors using the Abbott m2000sp™ automated sample preparation system. Viral loads measured using the RealTime™ HIV-1 (RealTime HIV-1) assay and the Abbott m2000rt™ instrument were compared to values obtained in the LCx® HIV RNA quantitative assay. Subtype was determined for 89 of 91 specimens by sequence/phylogenetic analysis of three genomic regions: gag p24, pol integrase, and env gp41. The panel included 69 subtype B, 1 C, 2 F, and 17 recombinant strains. Eighty-seven specimens were quantified by both assays. Two specimens were quantified only in RealTime HIV-1. Two additional specimens below the detection limit of both assays were also negative on PCR amplification. Viral load results were highly correlated, and good agreement was observed between assays with 90% of values within 0.5 log10 copies/ml. The RealTime HIV-1 assay and m2000 system offer the advantages of automation while providing reliable quantification of diverse HIV strains.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-08-23T11:03:09Z No. of bitstreams: 1 1-s2.0-S0166093406000395-main.pdf: 212674 bytes, checksum: 411c7cb3b1da7567c1b1dc64cd693538 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-16T13:11:50Z (GMT) No. of bitstreams: 1 1-s2.0-S0166093406000395-main.pdf: 212674 bytes, checksum: 411c7cb3b1da7567c1b1dc64cd693538 (MD5)Made available in DSpace on 2013-11-16T13:11:51Z (GMT). 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| dc.title.pt_BR.fl_str_mv |
Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil |
| dc.title.alternative.pt_BR.fl_str_mv |
Journal of Virological Methods |
| title |
Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil |
| spellingShingle |
Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil Brites, Carlos Real-time PCR HIV quantitation Genetic diversity |
| title_short |
Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil |
| title_full |
Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil |
| title_fullStr |
Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil |
| title_full_unstemmed |
Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil |
| title_sort |
Performance of the automated Abbott RealTime™ HIV-1 assay on a genetically diverse panel of specimens from Brazil |
| author |
Brites, Carlos |
| author_facet |
Brites, Carlos Swanson, Priscila Huang, Shihai Holzmayer, Vera Bodelle, Pierre Yamaguchi, Julie Brennan, Catherine A. Badaró, Roberto José da Silva Abravaya, Klara Devare, Sushil G. Hackett Junior, John |
| author_role |
author |
| author2 |
Swanson, Priscila Huang, Shihai Holzmayer, Vera Bodelle, Pierre Yamaguchi, Julie Brennan, Catherine A. Badaró, Roberto José da Silva Abravaya, Klara Devare, Sushil G. Hackett Junior, John |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Brites, Carlos Swanson, Priscila Huang, Shihai Holzmayer, Vera Bodelle, Pierre Yamaguchi, Julie Brennan, Catherine A. Badaró, Roberto José da Silva Abravaya, Klara Devare, Sushil G. Hackett Junior, John Brites, Carlos Swanson, Priscila Huang, Shihai Holzmayer, Vera Bodelle, Pierre Yamaguchi, Julie Brennan, Catherine A. Badaró, Roberto José da Silva Abravaya, Klara Devare, Sushil G. Hackett Junior, John |
| dc.subject.por.fl_str_mv |
Real-time PCR HIV quantitation Genetic diversity |
| topic |
Real-time PCR HIV quantitation Genetic diversity |
| description |
Texto completo: acesso restrito. p. 237–243 |
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2006 |
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2006 |
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2013-11-16T13:11:51Z |
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0166-0934 |
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v.134, n. 1-2 |
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