Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery

Detalhes bibliográficos
Autor(a) principal: Mehta, Sanjay R.
Data de Publicação: 2008
Outros Autores: Huang, Robert, Yang, Meng, Xing, Quan Zhang, Kolli, Bala, Kwang, Poo Chang, Badaró, Roberto José da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13902
Resumo: Texto completo: acesso restrito. p. 1764-1770
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spelling Mehta, Sanjay R.Huang, RobertYang, MengXing, Quan ZhangKolli, BalaKwang, Poo ChangBadaró, Roberto José da SilvaMehta, Sanjay R.Huang, RobertYang, MengXing, Quan ZhangKolli, BalaKwang, Poo ChangBadaró, Roberto José da Silva2013-11-26T13:03:04Z20081556-6811http://repositorio.ufba.br/ri/handle/ri/13902v. 15, n. 12Texto completo: acesso restrito. p. 1764-1770Leishmania species are obligate intracellular protozoan parasites that cause a broad spectrum of clinical diseases in mammalian hosts. The most frequently used approach to quantify parasites in murine model systems is based on thickness measurements of the footpad or ear after experimental infection. To overcome the limitations of this method, we used a Leishmania mutant episomally transfected with enhanced green fluorescent protein, enabling in vivo real-time whole-body fluorescence imaging, to follow the progression of Leishmania infection in parasitized tissues. Fluorescence correlated with the number of Leishmania parasites in the tissue and demonstrated the real-time efficacy of a therapeutic vaccine. This approach provides several substantial advantages over currently available animal model systems for the in vivo study of immunopathogenesis, prevention, and therapy of leishmaniasis. These include improvements in sensitivity and the ability to acquire real-time data on progression and spread of the infection.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-11-19T14:00:12Z No. of bitstreams: 1 10.1128CVI.00270-08.pdf: 656277 bytes, checksum: 8ec572e90b39d3c3ab885a579000b824 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-26T13:03:04Z (GMT) No. of bitstreams: 1 10.1128CVI.00270-08.pdf: 656277 bytes, checksum: 8ec572e90b39d3c3ab885a579000b824 (MD5)Made available in DSpace on 2013-11-26T13:03:04Z (GMT). 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dc.title.pt_BR.fl_str_mv Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
dc.title.alternative.pt_BR.fl_str_mv Clinical and Vaccine Immunology
title Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
spellingShingle Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
Mehta, Sanjay R.
title_short Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
title_full Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
title_fullStr Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
title_full_unstemmed Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
title_sort Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
author Mehta, Sanjay R.
author_facet Mehta, Sanjay R.
Huang, Robert
Yang, Meng
Xing, Quan Zhang
Kolli, Bala
Kwang, Poo Chang
Badaró, Roberto José da Silva
author_role author
author2 Huang, Robert
Yang, Meng
Xing, Quan Zhang
Kolli, Bala
Kwang, Poo Chang
Badaró, Roberto José da Silva
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mehta, Sanjay R.
Huang, Robert
Yang, Meng
Xing, Quan Zhang
Kolli, Bala
Kwang, Poo Chang
Badaró, Roberto José da Silva
Mehta, Sanjay R.
Huang, Robert
Yang, Meng
Xing, Quan Zhang
Kolli, Bala
Kwang, Poo Chang
Badaró, Roberto José da Silva
description Texto completo: acesso restrito. p. 1764-1770
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2013-11-26T13:03:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13902
dc.identifier.issn.none.fl_str_mv 1556-6811
dc.identifier.number.pt_BR.fl_str_mv v. 15, n. 12
identifier_str_mv 1556-6811
v. 15, n. 12
url http://repositorio.ufba.br/ri/handle/ri/13902
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1128/CVI.00270-08
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