Identification of differentially expressed genes in human prostate cancer using subtraction and microarray

Detalhes bibliográficos
Autor(a) principal: Xu, Jiangchun
Data de Publicação: 2000
Outros Autores: Stolk, John A., Silva, Sandra J., Houghton, Raymond L., Vedvick, Thomas S., Badaró, Roberto José da Silva, Reed, Steven G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7881
Resumo: Texto completo: acesso restrito. p.1677-1682
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spelling Xu, JiangchunStolk, John A.Silva, Sandra J.Houghton, Raymond L.Vedvick, Thomas S.Badaró, Roberto José da SilvaReed, Steven G.Xu, JiangchunStolk, John A.Silva, Sandra J.Houghton, Raymond L.Vedvick, Thomas S.Badaró, Roberto José da SilvaReed, Steven G.2013-01-16T12:05:58Z2000-03-150008-5472http://www.repositorio.ufba.br/ri/handle/ri/7881v. 60, n. 6Texto completo: acesso restrito. p.1677-1682We have identified human prostate cancer- and tissue-specific genes using cDNA library subtraction in conjunction with high throughput microarray screening. Subtracted cDNA libraries of prostate tumors and normal prostate tissue were generated. Characterization of subtracted libraries showed enrichment of both cancer- and tissue-specific genes. Highly redundant clones were eliminated by colony hybridization. The remaining clones were selected for microarray to determine gene expression levels in a variety of tumor and normal tissues. Clones showing overexpression in prostate tumors and/or normal prostate tissues were selected and sequenced. Here we report the identification of two genes, P503S and P504S, from subtracted libraries and a third gene, P510S, by subtraction followed by microarray screening. Their expression profiles were further confirmed by Northern blot, real-time PCR (TaqMan), and immunohistochemistry to be overexpressed in prostate tissues and/or prostate tumors. Full-length cDNA sequences were cloned, and their subcellular locations were predicted by a bioinformatic algorithm, PSORT, to be plasma membrane proteins. The genes identified through these approaches are potential candidates for cancer diagnosis and therapy.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2013-01-16T12:05:58Z No. of bitstreams: 1 Badaro.pdf: 3643192 bytes, checksum: d2d5e0c447ea86eb5fc23d840fc416d0 (MD5)Made available in DSpace on 2013-01-16T12:05:58Z (GMT). No. of bitstreams: 1 Badaro.pdf: 3643192 bytes, checksum: d2d5e0c447ea86eb5fc23d840fc416d0 (MD5) Previous issue date: 2000-03-15http://cancerres.aacrjournals.org/content/60/6/1677.full.pdf+htmlreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAIdentification of differentially expressed genes in human prostate cancer using subtraction and microarrayCancer Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINALBadaro.pdfBadaro.pdfapplication/pdf3643192https://repositorio.ufba.br/bitstream/ri/7881/1/Badaro.pdfd2d5e0c447ea86eb5fc23d840fc416d0MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7881/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTBadaro.pdf.txtBadaro.pdf.txtExtracted texttext/plain37188https://repositorio.ufba.br/bitstream/ri/7881/3/Badaro.pdf.txt40d6c41c375922df67b4c7363c939280MD53ri/78812022-07-05 14:03:34.017oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:34Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
dc.title.alternative.pt_BR.fl_str_mv Cancer Research
title Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
spellingShingle Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
Xu, Jiangchun
title_short Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
title_full Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
title_fullStr Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
title_full_unstemmed Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
title_sort Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
author Xu, Jiangchun
author_facet Xu, Jiangchun
Stolk, John A.
Silva, Sandra J.
Houghton, Raymond L.
Vedvick, Thomas S.
Badaró, Roberto José da Silva
Reed, Steven G.
author_role author
author2 Stolk, John A.
Silva, Sandra J.
Houghton, Raymond L.
Vedvick, Thomas S.
Badaró, Roberto José da Silva
Reed, Steven G.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Xu, Jiangchun
Stolk, John A.
Silva, Sandra J.
Houghton, Raymond L.
Vedvick, Thomas S.
Badaró, Roberto José da Silva
Reed, Steven G.
Xu, Jiangchun
Stolk, John A.
Silva, Sandra J.
Houghton, Raymond L.
Vedvick, Thomas S.
Badaró, Roberto José da Silva
Reed, Steven G.
description Texto completo: acesso restrito. p.1677-1682
publishDate 2000
dc.date.issued.fl_str_mv 2000-03-15
dc.date.accessioned.fl_str_mv 2013-01-16T12:05:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7881
dc.identifier.issn.none.fl_str_mv 0008-5472
dc.identifier.number.pt_BR.fl_str_mv v. 60, n. 6
identifier_str_mv 0008-5472
v. 60, n. 6
url http://www.repositorio.ufba.br/ri/handle/ri/7881
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://cancerres.aacrjournals.org/content/60/6/1677.full.pdf+html
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instname:Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
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