Identification of differentially expressed genes in human prostate cancer using subtraction and microarray
Autor(a) principal: | |
---|---|
Data de Publicação: | 2000 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/7881 |
Resumo: | Texto completo: acesso restrito. p.1677-1682 |
id |
UFBA-2_209715a01ea9e66e7e2ab32d7be1a1e6 |
---|---|
oai_identifier_str |
oai:repositorio.ufba.br:ri/7881 |
network_acronym_str |
UFBA-2 |
network_name_str |
Repositório Institucional da UFBA |
repository_id_str |
1932 |
spelling |
Xu, JiangchunStolk, John A.Silva, Sandra J.Houghton, Raymond L.Vedvick, Thomas S.Badaró, Roberto José da SilvaReed, Steven G.Xu, JiangchunStolk, John A.Silva, Sandra J.Houghton, Raymond L.Vedvick, Thomas S.Badaró, Roberto José da SilvaReed, Steven G.2013-01-16T12:05:58Z2000-03-150008-5472http://www.repositorio.ufba.br/ri/handle/ri/7881v. 60, n. 6Texto completo: acesso restrito. p.1677-1682We have identified human prostate cancer- and tissue-specific genes using cDNA library subtraction in conjunction with high throughput microarray screening. Subtracted cDNA libraries of prostate tumors and normal prostate tissue were generated. Characterization of subtracted libraries showed enrichment of both cancer- and tissue-specific genes. Highly redundant clones were eliminated by colony hybridization. The remaining clones were selected for microarray to determine gene expression levels in a variety of tumor and normal tissues. Clones showing overexpression in prostate tumors and/or normal prostate tissues were selected and sequenced. Here we report the identification of two genes, P503S and P504S, from subtracted libraries and a third gene, P510S, by subtraction followed by microarray screening. Their expression profiles were further confirmed by Northern blot, real-time PCR (TaqMan), and immunohistochemistry to be overexpressed in prostate tissues and/or prostate tumors. Full-length cDNA sequences were cloned, and their subcellular locations were predicted by a bioinformatic algorithm, PSORT, to be plasma membrane proteins. The genes identified through these approaches are potential candidates for cancer diagnosis and therapy.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2013-01-16T12:05:58Z No. of bitstreams: 1 Badaro.pdf: 3643192 bytes, checksum: d2d5e0c447ea86eb5fc23d840fc416d0 (MD5)Made available in DSpace on 2013-01-16T12:05:58Z (GMT). No. of bitstreams: 1 Badaro.pdf: 3643192 bytes, checksum: d2d5e0c447ea86eb5fc23d840fc416d0 (MD5) Previous issue date: 2000-03-15http://cancerres.aacrjournals.org/content/60/6/1677.full.pdf+htmlreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAIdentification of differentially expressed genes in human prostate cancer using subtraction and microarrayCancer Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINALBadaro.pdfBadaro.pdfapplication/pdf3643192https://repositorio.ufba.br/bitstream/ri/7881/1/Badaro.pdfd2d5e0c447ea86eb5fc23d840fc416d0MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7881/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTBadaro.pdf.txtBadaro.pdf.txtExtracted texttext/plain37188https://repositorio.ufba.br/bitstream/ri/7881/3/Badaro.pdf.txt40d6c41c375922df67b4c7363c939280MD53ri/78812022-07-05 14:03:34.017oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:34Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Identification of differentially expressed genes in human prostate cancer using subtraction and microarray |
dc.title.alternative.pt_BR.fl_str_mv |
Cancer Research |
title |
Identification of differentially expressed genes in human prostate cancer using subtraction and microarray |
spellingShingle |
Identification of differentially expressed genes in human prostate cancer using subtraction and microarray Xu, Jiangchun |
title_short |
Identification of differentially expressed genes in human prostate cancer using subtraction and microarray |
title_full |
Identification of differentially expressed genes in human prostate cancer using subtraction and microarray |
title_fullStr |
Identification of differentially expressed genes in human prostate cancer using subtraction and microarray |
title_full_unstemmed |
Identification of differentially expressed genes in human prostate cancer using subtraction and microarray |
title_sort |
Identification of differentially expressed genes in human prostate cancer using subtraction and microarray |
author |
Xu, Jiangchun |
author_facet |
Xu, Jiangchun Stolk, John A. Silva, Sandra J. Houghton, Raymond L. Vedvick, Thomas S. Badaró, Roberto José da Silva Reed, Steven G. |
author_role |
author |
author2 |
Stolk, John A. Silva, Sandra J. Houghton, Raymond L. Vedvick, Thomas S. Badaró, Roberto José da Silva Reed, Steven G. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Xu, Jiangchun Stolk, John A. Silva, Sandra J. Houghton, Raymond L. Vedvick, Thomas S. Badaró, Roberto José da Silva Reed, Steven G. Xu, Jiangchun Stolk, John A. Silva, Sandra J. Houghton, Raymond L. Vedvick, Thomas S. Badaró, Roberto José da Silva Reed, Steven G. |
description |
Texto completo: acesso restrito. p.1677-1682 |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000-03-15 |
dc.date.accessioned.fl_str_mv |
2013-01-16T12:05:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/7881 |
dc.identifier.issn.none.fl_str_mv |
0008-5472 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 60, n. 6 |
identifier_str_mv |
0008-5472 v. 60, n. 6 |
url |
http://www.repositorio.ufba.br/ri/handle/ri/7881 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.pt_BR.fl_str_mv |
http://cancerres.aacrjournals.org/content/60/6/1677.full.pdf+html |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
instname_str |
Universidade Federal da Bahia (UFBA) |
instacron_str |
UFBA |
institution |
UFBA |
reponame_str |
Repositório Institucional da UFBA |
collection |
Repositório Institucional da UFBA |
bitstream.url.fl_str_mv |
https://repositorio.ufba.br/bitstream/ri/7881/1/Badaro.pdf https://repositorio.ufba.br/bitstream/ri/7881/2/license.txt https://repositorio.ufba.br/bitstream/ri/7881/3/Badaro.pdf.txt |
bitstream.checksum.fl_str_mv |
d2d5e0c447ea86eb5fc23d840fc416d0 1b89a9a0548218172d7c829f87a0eab9 40d6c41c375922df67b4c7363c939280 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA) |
repository.mail.fl_str_mv |
|
_version_ |
1808459415801561088 |