Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines

Detalhes bibliográficos
Autor(a) principal: Moens, Britta
Data de Publicação: 2012
Outros Autores: Pannecouque, Christophe, López, Giovanni, Talledo, Michael, Gotuzzo, Eduardo, Cunha, Antônio Ricardo Khouri, Bittencourt, Achilea Candida Lisboa, Farré, Lourdes, Castro, Bernardo Galvão, Vandamme, Anne Mieke, Van Weyenbergh, Johan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15535
Resumo: p. 1-15
id UFBA-2_3a64fc7d630f66cc1940edb349db3c3d
oai_identifier_str oai:repositorio.ufba.br:ri/15535
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Moens, BrittaPannecouque, ChristopheLópez, GiovanniTalledo, MichaelGotuzzo, EduardoCunha, Antônio Ricardo KhouriBittencourt, Achilea Candida LisboaFarré, LourdesCastro, Bernardo GalvãoVandamme, Anne MiekeVan Weyenbergh, JohanMoens, BrittaPannecouque, ChristopheLópez, GiovanniTalledo, MichaelGotuzzo, EduardoCunha, Antônio Ricardo KhouriBittencourt, Achilea Candida LisboaFarré, LourdesCastro, Bernardo GalvãoVandamme, Anne MiekeVan Weyenbergh, Johan2014-08-11T15:38:05Z2014-08-11T15:38:05Z20121743-422Xhttp://repositorio.ufba.br/ri/handle/ri/15535v. 9, n. 171p. 1-15Background IFN-α contributes extensively to host immune response upon viral infection through antiviral, pro-apoptotic, antiproliferative and immunomodulatory activities. Although extensively documented in various types of human cancers and viral infections, controversy exists in the exact mechanism of action of IFN-α in human immunodeficiency virus type 1 (HIV-1) and human T-lymphotropic virus type 1 (HTLV-1) retroviral infections. Results IFN-α displayed strong anti-HIV-1 effects in HIV-1/HTLV-1 co-infected MT-4 cells in vitro, demonstrated by the dose-dependent inhibition of the HIV-1-induced cytopathic effect (IC50 = 83.5 IU/ml, p < 0.0001) and p24 levels in cell-free supernatant (IC50 = 1.2 IU/ml, p < 0.0001). In contrast, IFN-α treatment did not affect cell viability or HTLV-1 viral mRNA levels in HTLV-1 mono-infected cell lines, based on flow cytometry and nCounter analysis, respectively. However, we were able to confirm the previously described post-transcriptional inhibition of HTLV-1 p19 secretion by IFN-α in cell lines (p = 0.0045), and extend this finding to primary Adult T cell Leukemia patient samples (p = 0.031). In addition, through microarray and nCounter analysis, we performed the first genome-wide simultaneous quantification of complete human and retroviral transciptomes, demonstrating significant transcriptional activation of interferon-stimulated genes without concomitant decrease of HTLV-1 mRNA levels. Conclusions Taken together, our results indicate that both the absence of in vitro antiproliferative and pro-apoptotic activity as well as the modest post-transcriptional antiviral activity of IFN-α against HTLV-1, were not due to a cell-intrinsic defect in IFN-α signalisation, but rather represents a retrovirus-specific phenomenon, considering the strong HIV-1 inhibition in co-infected cells.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-07-16T11:52:51Z No. of bitstreams: 1 Achiléa Bittencourt.pdf: 1561034 bytes, checksum: 891e9491b8c19acf3073a5c4c4d4eecf (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-08-11T15:38:05Z (GMT) No. of bitstreams: 1 Achiléa Bittencourt.pdf: 1561034 bytes, checksum: 891e9491b8c19acf3073a5c4c4d4eecf (MD5)Made available in DSpace on 2014-08-11T15:38:05Z (GMT). No. of bitstreams: 1 Achiléa Bittencourt.pdf: 1561034 bytes, checksum: 891e9491b8c19acf3073a5c4c4d4eecf (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1186/1743-422X-9-171reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBARetrovirusIFN-αHIV-1HTLV-1IFN-α signalingAntiviral activitySimultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell linesVirology Journalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINALAchiléa Bittencourt.pdfAchiléa Bittencourt.pdfapplication/pdf1561034https://repositorio.ufba.br/bitstream/ri/15535/1/Achil%c3%a9a%20Bittencourt.pdf891e9491b8c19acf3073a5c4c4d4eecfMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15535/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTAchiléa Bittencourt.pdf.txtAchiléa Bittencourt.pdf.txtExtracted texttext/plain64632https://repositorio.ufba.br/bitstream/ri/15535/3/Achil%c3%a9a%20Bittencourt.pdf.txt630098110d80450446d6b21d53ecd14bMD53ri/155352022-08-08 13:19:12.885oai:repositorio.ufba.br:ri/15535VGVybW8gZGUgTGljZW4/YSwgbj9vIGV4Y2x1c2l2bywgcGFyYSBvIGRlcD9zaXRvIG5vIFJlcG9zaXQ/cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZCQS4KCiBQZWxvIHByb2Nlc3NvIGRlIHN1Ym1pc3M/byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldSByZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIAplc3NlIHRlcm1vIGRlIGxpY2VuP2EsIGNvbmNlZGUgYW8gUmVwb3NpdD9yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSAKbyBkaXJlaXRvIGRlIG1hbnRlciB1bWEgYz9waWEgZW0gc2V1IHJlcG9zaXQ/cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCBkZSBwcmVzZXJ2YT8/by4gCkVzc2VzIHRlcm1vcywgbj9vIGV4Y2x1c2l2b3MsIG1hbnQ/bSBvcyBkaXJlaXRvcyBkZSBhdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIApjb21vIHBhcnRlIGRvIGFjZXJ2byBpbnRlbGVjdHVhbCBkZXNzYSBVbml2ZXJzaWRhZGUuCgogUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVpPz9vLCBlc3NlIHRlcm1vIGRlIGxpY2VuP2EgCmVudGVuZGUgcXVlOgoKIE1hbnRlbmRvIG9zIGRpcmVpdG9zIGF1dG9yYWlzLCByZXBhc3NhZG9zIGEgdGVyY2Vpcm9zLCBlbSBjYXNvIGRlIHB1YmxpY2E/P2VzLCBvIHJlcG9zaXQ/cmlvCnBvZGUgcmVzdHJpbmdpciBvIGFjZXNzbyBhbyB0ZXh0byBpbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1hPz9lcyBzb2JyZSBvIGRvY3VtZW50bwooTWV0YWRhZG9zIGVzY3JpdGl2b3MpLgoKIERlc3RhIGZvcm1hLCBhdGVuZGVuZG8gYW9zIGFuc2Vpb3MgZGVzc2EgdW5pdmVyc2lkYWRlIGVtIG1hbnRlciBzdWEgcHJvZHU/P28gY2llbnQ/ZmljYSBjb20gCmFzIHJlc3RyaT8/ZXMgaW1wb3N0YXMgcGVsb3MgZWRpdG9yZXMgZGUgcGVyaT9kaWNvcy4KCiBQYXJhIGFzIHB1YmxpY2E/P2VzIHNlbSBpbmljaWF0aXZhcyBxdWUgc2VndWVtIGEgcG9sP3RpY2EgZGUgQWNlc3NvIEFiZXJ0bywgb3MgZGVwP3NpdG9zIApjb21wdWxzP3Jpb3MgbmVzc2UgcmVwb3NpdD9yaW8gbWFudD9tIG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudD9tIGFjZXNzbyBpcnJlc3RyaXRvIAphbyBtZXRhZGFkb3MgZSB0ZXh0byBjb21wbGV0by4gQXNzaW0sIGEgYWNlaXRhPz9vIGRlc3NlIHRlcm1vIG4/byBuZWNlc3NpdGEgZGUgY29uc2VudGltZW50bwogcG9yIHBhcnRlIGRlIGF1dG9yZXMvZGV0ZW50b3JlcyBkb3MgZGlyZWl0b3MsIHBvciBlc3RhcmVtIGVtIGluaWNpYXRpdmFzIGRlIGFjZXNzbyBhYmVydG8uCg==Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:19:12Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
dc.title.alternative.pt_BR.fl_str_mv Virology Journal
title Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
spellingShingle Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
Moens, Britta
Retrovirus
IFN-α
HIV-1
HTLV-1
IFN-α signaling
Antiviral activity
title_short Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
title_full Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
title_fullStr Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
title_full_unstemmed Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
title_sort Simultaneous RNA quantification of human and retroviral genomes reveals intact interferon signaling in HTLV-1-infected CD4+ T cell lines
author Moens, Britta
author_facet Moens, Britta
Pannecouque, Christophe
López, Giovanni
Talledo, Michael
Gotuzzo, Eduardo
Cunha, Antônio Ricardo Khouri
Bittencourt, Achilea Candida Lisboa
Farré, Lourdes
Castro, Bernardo Galvão
Vandamme, Anne Mieke
Van Weyenbergh, Johan
author_role author
author2 Pannecouque, Christophe
López, Giovanni
Talledo, Michael
Gotuzzo, Eduardo
Cunha, Antônio Ricardo Khouri
Bittencourt, Achilea Candida Lisboa
Farré, Lourdes
Castro, Bernardo Galvão
Vandamme, Anne Mieke
Van Weyenbergh, Johan
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moens, Britta
Pannecouque, Christophe
López, Giovanni
Talledo, Michael
Gotuzzo, Eduardo
Cunha, Antônio Ricardo Khouri
Bittencourt, Achilea Candida Lisboa
Farré, Lourdes
Castro, Bernardo Galvão
Vandamme, Anne Mieke
Van Weyenbergh, Johan
Moens, Britta
Pannecouque, Christophe
López, Giovanni
Talledo, Michael
Gotuzzo, Eduardo
Cunha, Antônio Ricardo Khouri
Bittencourt, Achilea Candida Lisboa
Farré, Lourdes
Castro, Bernardo Galvão
Vandamme, Anne Mieke
Van Weyenbergh, Johan
dc.subject.por.fl_str_mv Retrovirus
IFN-α
HIV-1
HTLV-1
IFN-α signaling
Antiviral activity
topic Retrovirus
IFN-α
HIV-1
HTLV-1
IFN-α signaling
Antiviral activity
description p. 1-15
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-08-11T15:38:05Z
dc.date.available.fl_str_mv 2014-08-11T15:38:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15535
dc.identifier.issn.none.fl_str_mv 1743-422X
dc.identifier.number.pt_BR.fl_str_mv v. 9, n. 171
identifier_str_mv 1743-422X
v. 9, n. 171
url http://repositorio.ufba.br/ri/handle/ri/15535
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1186/1743-422X-9-171
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/15535/1/Achil%c3%a9a%20Bittencourt.pdf
https://repositorio.ufba.br/bitstream/ri/15535/2/license.txt
https://repositorio.ufba.br/bitstream/ri/15535/3/Achil%c3%a9a%20Bittencourt.pdf.txt
bitstream.checksum.fl_str_mv 891e9491b8c19acf3073a5c4c4d4eecf
0d4b811ef71182510d2015daa7c8a900
630098110d80450446d6b21d53ecd14b
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459480747212800