Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity

Detalhes bibliográficos
Autor(a) principal: Oliveira, Diêgo Madureira de
Data de Publicação: 2011
Outros Autores: Alvarez, Lisandro Diego Giraldez, Barreto, George Emílio Sampaio, Galeano, Pablo, Romero, Juan Ignacio, Holubiec, Mariana Inés, Badorrey, Maria Sol, Capani, Francisco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14747
Resumo: Texto completo: acesso restrito. p. 1382-1391
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spelling Oliveira, Diêgo Madureira deAlvarez, Lisandro Diego GiraldezBarreto, George Emílio SampaioGaleano, PabloRomero, Juan IgnacioHolubiec, Mariana InésBadorrey, Maria SolCapani, FranciscoOliveira, Diêgo Madureira deAlvarez, Lisandro Diego GiraldezBarreto, George Emílio SampaioGaleano, PabloRomero, Juan IgnacioHolubiec, Mariana InésBadorrey, Maria SolCapani, Francisco2014-03-13T18:22:39Z20110960-3271http://repositorio.ufba.br/ri/handle/ri/14747v. 30, n. 9Texto completo: acesso restrito. p. 1382-1391Paullinia cupana Mart. var. Sorbilis, commonly known as Guaraná, is a Brazilian plant frequently cited for its antioxidant properties and different pharmacological activities on the central nervous system. The potential beneficial uses of Guaraná in neurodegenerative disorders, such as in Parkinson's disease (PD), the pathogenesis of which is associated with mitochondrial dysfunction and oxidative stress, has not yet been assessed. Therefore, the main aim of the present study was to evaluate if an extract of commercial powdered seeds of Guaraná could protect human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity. Two concentration of Guaraná dimethylsulfoxide extract (0.312 and 0.625 mg/mL) were added to SH-SY5Y cells treated with 300 nM rotenone for 48 h, and the cytoprotective effects were assessed by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, measuring lactate dehydrogenase (LDH) levels, and analyzing nuclear integrity with Hoechst33258 stain. Results showed that the addition of Guaraná extract significantly increased the cell viability of SH-SY5Y cells treated with rotenone, in a dose-dependent manner. On the other hand, LDH levels were significantly reduced by addition of 0.312 mg/mL of Guaraná, but unexpectedly, no changes were observed with the higher concentration. Moreover, chromatin condensation and nuclear fragmentation were significantly reduced by addition of any of both concentrations of the extract. The results obtained in this work could provide relevant information about the mechanisms underlying the degeneration of dopaminergic neurons in PD and precede in vivo experiments. Further studies are needed to investigate which active constituent is responsible for the cytoprotective effect produced by Paullinia cupana.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-02-25T12:19:45Z No. of bitstreams: 1 Diêgo Madureira de Oliveira.pdf: 452860 bytes, checksum: 5ca98db87d78c11736675c97672ffef6 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-03-13T18:22:39Z (GMT) No. of bitstreams: 1 Diêgo Madureira de Oliveira.pdf: 452860 bytes, checksum: 5ca98db87d78c11736675c97672ffef6 (MD5)Made available in DSpace on 2014-03-13T18:22:39Z (GMT). No. of bitstreams: 1 Diêgo Madureira de Oliveira.pdf: 452860 bytes, checksum: 5ca98db87d78c11736675c97672ffef6 (MD5) Previous issue date: 2011http://dx.doi.org/ 10.1177/0960327110389837reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAParkinson's diseaseSH-SY5Y cellsGuaranáRotenoneCytotoxicityNeuroprotectionAntioxidant activityPaullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicityHuman and Experimental Toxicologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALDiêgo Madureira de Oliveira.pdfDiêgo Madureira de Oliveira.pdfapplication/pdf452860https://repositorio.ufba.br/bitstream/ri/14747/1/Di%c3%aago%20Madureira%20de%20Oliveira.pdf5ca98db87d78c11736675c97672ffef6MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14747/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTDiêgo Madureira de Oliveira.pdf.txtDiêgo Madureira de Oliveira.pdf.txtExtracted texttext/plain43702https://repositorio.ufba.br/bitstream/ri/14747/3/Di%c3%aago%20Madureira%20de%20Oliveira.pdf.txta22b4607d32a3f54c73e2c2dde89c22aMD53ri/147472022-07-05 14:04:14.607oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:04:14Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
dc.title.alternative.pt_BR.fl_str_mv Human and Experimental Toxicology
title Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
spellingShingle Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
Oliveira, Diêgo Madureira de
Parkinson's disease
SH-SY5Y cells
Guaraná
Rotenone
Cytotoxicity
Neuroprotection
Antioxidant activity
title_short Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
title_full Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
title_fullStr Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
title_full_unstemmed Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
title_sort Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
author Oliveira, Diêgo Madureira de
author_facet Oliveira, Diêgo Madureira de
Alvarez, Lisandro Diego Giraldez
Barreto, George Emílio Sampaio
Galeano, Pablo
Romero, Juan Ignacio
Holubiec, Mariana Inés
Badorrey, Maria Sol
Capani, Francisco
author_role author
author2 Alvarez, Lisandro Diego Giraldez
Barreto, George Emílio Sampaio
Galeano, Pablo
Romero, Juan Ignacio
Holubiec, Mariana Inés
Badorrey, Maria Sol
Capani, Francisco
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Diêgo Madureira de
Alvarez, Lisandro Diego Giraldez
Barreto, George Emílio Sampaio
Galeano, Pablo
Romero, Juan Ignacio
Holubiec, Mariana Inés
Badorrey, Maria Sol
Capani, Francisco
Oliveira, Diêgo Madureira de
Alvarez, Lisandro Diego Giraldez
Barreto, George Emílio Sampaio
Galeano, Pablo
Romero, Juan Ignacio
Holubiec, Mariana Inés
Badorrey, Maria Sol
Capani, Francisco
dc.subject.por.fl_str_mv Parkinson's disease
SH-SY5Y cells
Guaraná
Rotenone
Cytotoxicity
Neuroprotection
Antioxidant activity
topic Parkinson's disease
SH-SY5Y cells
Guaraná
Rotenone
Cytotoxicity
Neuroprotection
Antioxidant activity
description Texto completo: acesso restrito. p. 1382-1391
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-03-13T18:22:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14747
dc.identifier.issn.none.fl_str_mv 0960-3271
dc.identifier.number.pt_BR.fl_str_mv v. 30, n. 9
identifier_str_mv 0960-3271
v. 30, n. 9
url http://repositorio.ufba.br/ri/handle/ri/14747
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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