Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells

Detalhes bibliográficos
Autor(a) principal: Andrade, Deyse Valverde Gomes de
Data de Publicação: 2008
Outros Autores: Oliveria, Diêgo Madureira de, Barreto, George Emílio Sampaio, Bertolino, Laura-Aon, Saraceno, Ezequiel, Capani, Francisco, Giraldez, Lisandro Diego
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6348
Resumo: Acesso restrito: Texto completo. p. 188-196
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spelling Andrade, Deyse Valverde Gomes deOliveria, Diêgo Madureira deBarreto, George Emílio SampaioBertolino, Laura-AonSaraceno, EzequielCapani, FranciscoGiraldez, Lisandro DiegoAndrade, Deyse Valverde Gomes deOliveria, Diêgo Madureira deBarreto, George Emílio SampaioBertolino, Laura-AonSaraceno, EzequielCapani, FranciscoGiraldez, Lisandro Diego2012-07-05T19:08:33Z20080006-8993http://www.repositorio.ufba.br/ri/handle/ri/6348v. 1198Acesso restrito: Texto completo. p. 188-196Parkinson's disease (PD) is one of the most important neurodegenerativeworldwide disorders. It is characterized by a selective and progressive degeneration of dopaminergic neurons, causing a series of symptoms which might ultimately induce programmed cell death. The potential cytoprotective effects of one of the commercial extracts of Anemopaegma mirandum (Catuaba), a Brazilian tree, on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells was demonstrated. The cell viability, analysis of cellular morphology, nuclei morphology and ultra structural research were done by MTT-tetrazole (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) assay, phase contrast microscopy, stained with Hoechst 33258 and electron microscopy transmission, respectively. Three different concentrations of Catuaba extract were used (0.312, 0.625 and 1.250 mg/mL). These extracts promoted an increase of 22.3± 3.6%, 22.0±2.1% and 15.8±0.7% on the cell viability. Notable changes in the cellularmorphology, condensation of the cell body, nuclear fragmentation and condensation into discrete dense chromatin clumps were observed when the cells were treated with 300 nM Rotenone for 48 h. These effectswere partially alteredwhen the extract of A. mirandum was added to the Rotenone treatment. Ultra structural analysis by electron microscopy demonstrated that citoplasmatic membranes and mitochondria membrane were also clearly preserved in the group treatedwith the extract. Therefore, in this study, our findings indicated that extracts of A. mirandum have cytoprotective effects on Rotenone-induced apoptosis in humanneuroblastomas SH-SY5Y cells.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-07-05T19:08:33Z No. of bitstreams: 1 CONFIG~1....0-S0006899308000346-main.pdf: 461689 bytes, checksum: a281cf3ad3f9bcfbbe293b5952e4dd24 (MD5)Made available in DSpace on 2012-07-05T19:08:33Z (GMT). No. of bitstreams: 1 CONFIG~1....0-S0006899308000346-main.pdf: 461689 bytes, checksum: a281cf3ad3f9bcfbbe293b5952e4dd24 (MD5) Previous issue date: 2008http://dx.doi.org/10.1016/j.brainres.2008.01.006reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAParkinson diseaseSH-SY5Y cellsCatuabaCytoprotectiveRotenoneEffects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cellsBrain Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINALCONFIG~1....0-S0006899308000346-main.pdfCONFIG~1....0-S0006899308000346-main.pdfapplication/pdf461689https://repositorio.ufba.br/bitstream/ri/6348/1/CONFIG~1....0-S0006899308000346-main.pdfa281cf3ad3f9bcfbbe293b5952e4dd24MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6348/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTCONFIG~1....0-S0006899308000346-main.pdf.txtCONFIG~1....0-S0006899308000346-main.pdf.txtExtracted texttext/plain39872https://repositorio.ufba.br/bitstream/ri/6348/3/CONFIG~1....0-S0006899308000346-main.pdf.txtacf262ee334f3c2e3fce26c949d1af18MD53ri/63482022-07-05 14:03:21.866oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:21Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells
dc.title.alternative.pt_BR.fl_str_mv Brain Research
title Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells
spellingShingle Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells
Andrade, Deyse Valverde Gomes de
Parkinson disease
SH-SY5Y cells
Catuaba
Cytoprotective
Rotenone
title_short Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells
title_full Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells
title_fullStr Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells
title_full_unstemmed Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells
title_sort Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells
author Andrade, Deyse Valverde Gomes de
author_facet Andrade, Deyse Valverde Gomes de
Oliveria, Diêgo Madureira de
Barreto, George Emílio Sampaio
Bertolino, Laura-Aon
Saraceno, Ezequiel
Capani, Francisco
Giraldez, Lisandro Diego
author_role author
author2 Oliveria, Diêgo Madureira de
Barreto, George Emílio Sampaio
Bertolino, Laura-Aon
Saraceno, Ezequiel
Capani, Francisco
Giraldez, Lisandro Diego
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Deyse Valverde Gomes de
Oliveria, Diêgo Madureira de
Barreto, George Emílio Sampaio
Bertolino, Laura-Aon
Saraceno, Ezequiel
Capani, Francisco
Giraldez, Lisandro Diego
Andrade, Deyse Valverde Gomes de
Oliveria, Diêgo Madureira de
Barreto, George Emílio Sampaio
Bertolino, Laura-Aon
Saraceno, Ezequiel
Capani, Francisco
Giraldez, Lisandro Diego
dc.subject.por.fl_str_mv Parkinson disease
SH-SY5Y cells
Catuaba
Cytoprotective
Rotenone
topic Parkinson disease
SH-SY5Y cells
Catuaba
Cytoprotective
Rotenone
description Acesso restrito: Texto completo. p. 188-196
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-07-05T19:08:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6348
dc.identifier.issn.none.fl_str_mv 0006-8993
dc.identifier.number.pt_BR.fl_str_mv v. 1198
identifier_str_mv 0006-8993
v. 1198
url http://www.repositorio.ufba.br/ri/handle/ri/6348
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.brainres.2008.01.006
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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