Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/14747 |
Resumo: | Texto completo: acesso restrito. p. 1382-1391 |
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Oliveira, Diêgo Madureira deAlvarez, Lisandro Diego GiraldezBarreto, George Emílio SampaioGaleano, PabloRomero, Juan IgnacioHolubiec, Mariana InésBadorrey, Maria SolCapani, FranciscoOliveira, Diêgo Madureira deAlvarez, Lisandro Diego GiraldezBarreto, George Emílio SampaioGaleano, PabloRomero, Juan IgnacioHolubiec, Mariana InésBadorrey, Maria SolCapani, Francisco2014-03-13T18:22:39Z20110960-3271http://repositorio.ufba.br/ri/handle/ri/14747v. 30, n. 9Texto completo: acesso restrito. p. 1382-1391Paullinia cupana Mart. var. Sorbilis, commonly known as Guaraná, is a Brazilian plant frequently cited for its antioxidant properties and different pharmacological activities on the central nervous system. The potential beneficial uses of Guaraná in neurodegenerative disorders, such as in Parkinson's disease (PD), the pathogenesis of which is associated with mitochondrial dysfunction and oxidative stress, has not yet been assessed. Therefore, the main aim of the present study was to evaluate if an extract of commercial powdered seeds of Guaraná could protect human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity. Two concentration of Guaraná dimethylsulfoxide extract (0.312 and 0.625 mg/mL) were added to SH-SY5Y cells treated with 300 nM rotenone for 48 h, and the cytoprotective effects were assessed by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, measuring lactate dehydrogenase (LDH) levels, and analyzing nuclear integrity with Hoechst33258 stain. Results showed that the addition of Guaraná extract significantly increased the cell viability of SH-SY5Y cells treated with rotenone, in a dose-dependent manner. On the other hand, LDH levels were significantly reduced by addition of 0.312 mg/mL of Guaraná, but unexpectedly, no changes were observed with the higher concentration. Moreover, chromatin condensation and nuclear fragmentation were significantly reduced by addition of any of both concentrations of the extract. The results obtained in this work could provide relevant information about the mechanisms underlying the degeneration of dopaminergic neurons in PD and precede in vivo experiments. Further studies are needed to investigate which active constituent is responsible for the cytoprotective effect produced by Paullinia cupana.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-02-25T12:19:45Z No. of bitstreams: 1 Diêgo Madureira de Oliveira.pdf: 452860 bytes, checksum: 5ca98db87d78c11736675c97672ffef6 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-03-13T18:22:39Z (GMT) No. of bitstreams: 1 Diêgo Madureira de Oliveira.pdf: 452860 bytes, checksum: 5ca98db87d78c11736675c97672ffef6 (MD5)Made available in DSpace on 2014-03-13T18:22:39Z (GMT). No. of bitstreams: 1 Diêgo Madureira de Oliveira.pdf: 452860 bytes, checksum: 5ca98db87d78c11736675c97672ffef6 (MD5) Previous issue date: 2011http://dx.doi.org/ 10.1177/0960327110389837reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAParkinson's diseaseSH-SY5Y cellsGuaranáRotenoneCytotoxicityNeuroprotectionAntioxidant activityPaullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicityHuman and Experimental Toxicologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALDiêgo Madureira de Oliveira.pdfDiêgo Madureira de Oliveira.pdfapplication/pdf452860https://repositorio.ufba.br/bitstream/ri/14747/1/Di%c3%aago%20Madureira%20de%20Oliveira.pdf5ca98db87d78c11736675c97672ffef6MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14747/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTDiêgo Madureira de Oliveira.pdf.txtDiêgo Madureira de Oliveira.pdf.txtExtracted texttext/plain43702https://repositorio.ufba.br/bitstream/ri/14747/3/Di%c3%aago%20Madureira%20de%20Oliveira.pdf.txta22b4607d32a3f54c73e2c2dde89c22aMD53ri/147472022-07-05 14:04:14.607oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:04:14Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity |
dc.title.alternative.pt_BR.fl_str_mv |
Human and Experimental Toxicology |
title |
Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity |
spellingShingle |
Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity Oliveira, Diêgo Madureira de Parkinson's disease SH-SY5Y cells Guaraná Rotenone Cytotoxicity Neuroprotection Antioxidant activity |
title_short |
Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity |
title_full |
Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity |
title_fullStr |
Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity |
title_full_unstemmed |
Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity |
title_sort |
Paullinia cupana Mart. var. Sorbilis protects human dopaminergic neuroblastoma SH-SY5Y cell line against rotenone-induced cytotoxicity |
author |
Oliveira, Diêgo Madureira de |
author_facet |
Oliveira, Diêgo Madureira de Alvarez, Lisandro Diego Giraldez Barreto, George Emílio Sampaio Galeano, Pablo Romero, Juan Ignacio Holubiec, Mariana Inés Badorrey, Maria Sol Capani, Francisco |
author_role |
author |
author2 |
Alvarez, Lisandro Diego Giraldez Barreto, George Emílio Sampaio Galeano, Pablo Romero, Juan Ignacio Holubiec, Mariana Inés Badorrey, Maria Sol Capani, Francisco |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Diêgo Madureira de Alvarez, Lisandro Diego Giraldez Barreto, George Emílio Sampaio Galeano, Pablo Romero, Juan Ignacio Holubiec, Mariana Inés Badorrey, Maria Sol Capani, Francisco Oliveira, Diêgo Madureira de Alvarez, Lisandro Diego Giraldez Barreto, George Emílio Sampaio Galeano, Pablo Romero, Juan Ignacio Holubiec, Mariana Inés Badorrey, Maria Sol Capani, Francisco |
dc.subject.por.fl_str_mv |
Parkinson's disease SH-SY5Y cells Guaraná Rotenone Cytotoxicity Neuroprotection Antioxidant activity |
topic |
Parkinson's disease SH-SY5Y cells Guaraná Rotenone Cytotoxicity Neuroprotection Antioxidant activity |
description |
Texto completo: acesso restrito. p. 1382-1391 |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2014-03-13T18:22:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/14747 |
dc.identifier.issn.none.fl_str_mv |
0960-3271 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 30, n. 9 |
identifier_str_mv |
0960-3271 v. 30, n. 9 |
url |
http://repositorio.ufba.br/ri/handle/ri/14747 |
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eng |
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eng |
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