Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/12392 |
Resumo: | Texto completo. Acesso restrito. p. 269–274 |
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Jesus, E. E. V.Pinheiro, A. M.Santos, A. B.Freire, S. M.Tardy, Marcienne BlochEl-Bachá, Ramon dos SantosCosta, Silvia LimaCosta, Maria de Fátima DiasJesus, E. E. V.Pinheiro, A. M.Santos, A. B.Freire, S. M.Tardy, Marcienne BlochEl-Bachá, Ramon dos SantosCosta, Silvia LimaCosta, Maria de Fátima Dias2013-07-31T20:21:54Z20130014-4894http://www.repositorio.ufba.br/ri/handle/ri/12392v. 133, n. 3Texto completo. Acesso restrito. p. 269–274Neospora caninum causes abortion in cattle and neurological disorders in dogs. The immunological response to this parasite has been described as predominantly of the Th1 type. However, infected primary glial cell cultures release IL-10 and IL-6 but not IFN-γ. This suggests a rather protective response of the glia to avoid inflammatory damage of the nervous tissue. In this study, we investigated the effects of pro-inflammatory cytokines in primary mixed cultures of rat astrocytes and microglia infected with N. caninum. The cells were treated with either IFN-γ, TNF-α, anti-IL-10 or anti-TGF-β antibodies and were infected with parasite tachyzoites 24 h later. Trypan Blue exclusion and MTT assays were performed to test cell viability. It was observed that cytokines, antibody treatment and in vitro infection did not reveal significant cell death in the various culture conditions. Treatment with 50, 150 and 300 IU/mL of either IFN-γ or TNF-α reduced tachyzoites numbers in cultures by 36.7%, 54.8% and 63.8% for IFN-γ and by 27.6%, 38.4% and 29.7% for TNF-α, respectively. In the absence of IL-10 and TGF-β, tachyzoite numbers were reduced by 52.8% and 41.5%, respectively. While IFN-γ (150 and 300 IU/mL) increased the nitrite levels in uninfected cells, parasite infection seemed to reduce the nitrite levels, and this reduction was more expressive in IFN-γ-infected cells, thereby suggesting an inhibitory effect on its production. However, TNF-α, IL-10 and TGF-β did not affect the nitrite levels. Basal PGE2 levels also increased by 17% and 25%; 78% and 13% in uninfected and infected cells treated with IFN-γ or anti-TGF-β, respectively. Nevertheless, the antibody neutralization of IL-10 reduced PGE2 release significantly. These results highlight the possibility of a combined effect between the IFN-γ and parasite evasion strategies and show that the IFN-γ, TNF-α, IL-10 and TGF-β cytokines participate in parasite proliferation control mechanisms.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-06-26T14:27:59Z No. of bitstreams: 1 777777777777777.pdf: 501860 bytes, checksum: 84a13f9601a2c559d7ec8644494829fe (MD5)Approved for entry into archive by Flávia Ferreira(flaviaccf@yahoo.com.br) on 2013-07-31T20:21:54Z (GMT) No. of bitstreams: 1 777777777777777.pdf: 501860 bytes, checksum: 84a13f9601a2c559d7ec8644494829fe (MD5)Made available in DSpace on 2013-07-31T20:21:54Z (GMT). No. of bitstreams: 1 777777777777777.pdf: 501860 bytes, checksum: 84a13f9601a2c559d7ec8644494829fe (MD5) Previous issue date: 2013SalvadorExperimental Parasitologyhttp://dx.doi.org/10.1016/j.exppara.2012.11.016reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBANeospora caninumMicrogliaImmune responseEffects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cellsExperimental Parasitologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12392/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINAL777777777777777.pdf777777777777777.pdfapplication/pdf501860https://repositorio.ufba.br/bitstream/ri/12392/1/777777777777777.pdf84a13f9601a2c559d7ec8644494829feMD51TEXT777777777777777.pdf.txt777777777777777.pdf.txtExtracted texttext/plain35847https://repositorio.ufba.br/bitstream/ri/12392/3/777777777777777.pdf.txte8557e8ea5334f900350ce3d5cd620a8MD53ri/123922022-08-08 13:18:18.901oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:18:18Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells |
| dc.title.alternative.pt_BR.fl_str_mv |
Experimental Parasitology |
| title |
Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells |
| spellingShingle |
Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells Jesus, E. E. V. Neospora caninum Microglia Immune response |
| title_short |
Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells |
| title_full |
Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells |
| title_fullStr |
Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells |
| title_full_unstemmed |
Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells |
| title_sort |
Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells |
| author |
Jesus, E. E. V. |
| author_facet |
Jesus, E. E. V. Pinheiro, A. M. Santos, A. B. Freire, S. M. Tardy, Marcienne Bloch El-Bachá, Ramon dos Santos Costa, Silvia Lima Costa, Maria de Fátima Dias |
| author_role |
author |
| author2 |
Pinheiro, A. M. Santos, A. B. Freire, S. M. Tardy, Marcienne Bloch El-Bachá, Ramon dos Santos Costa, Silvia Lima Costa, Maria de Fátima Dias |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Jesus, E. E. V. Pinheiro, A. M. Santos, A. B. Freire, S. M. Tardy, Marcienne Bloch El-Bachá, Ramon dos Santos Costa, Silvia Lima Costa, Maria de Fátima Dias Jesus, E. E. V. Pinheiro, A. M. Santos, A. B. Freire, S. M. Tardy, Marcienne Bloch El-Bachá, Ramon dos Santos Costa, Silvia Lima Costa, Maria de Fátima Dias |
| dc.subject.por.fl_str_mv |
Neospora caninum Microglia Immune response |
| topic |
Neospora caninum Microglia Immune response |
| description |
Texto completo. Acesso restrito. p. 269–274 |
| publishDate |
2013 |
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2013-07-31T20:21:54Z |
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2013 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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0014-4894 |
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v. 133, n. 3 |
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0014-4894 v. 133, n. 3 |
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http://www.repositorio.ufba.br/ri/handle/ri/12392 |
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Experimental Parasitology |
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Experimental Parasitology |
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http://dx.doi.org/10.1016/j.exppara.2012.11.016 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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