Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis

Detalhes bibliográficos
Autor(a) principal: Rossi, Eglas Emanuel
Data de Publicação: 2012
Outros Autores: Pinheiro, Antonio Luiz Barbosa, Baltatu, Ovidiu C., Pacheco, Marcos Tadeu Tavares, Silveira Jr., Landulfo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/16368
Resumo: Texto completo: acesso restrito. p. 73–78
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spelling Rossi, Eglas EmanuelPinheiro, Antonio Luiz BarbosaBaltatu, Ovidiu C.Pacheco, Marcos Tadeu TavaresSilveira Jr., LandulfoRossi, Eglas EmanuelPinheiro, Antonio Luiz BarbosaBaltatu, Ovidiu C.Pacheco, Marcos Tadeu TavaresSilveira Jr., Landulfo2014-10-10T14:22:12Z20121011-1344http://repositorio.ufba.br/ri/handle/ri/16368v. 107, n. 6Texto completo: acesso restrito. p. 73–78Raman spectroscopy has been used for the diagnosis of various eye diseases. A diagnostic tool based on Raman spectroscopy has been developed to discriminate endophthalmitis from uveitis in vitreous tissues of rabbits’ eyes in vitro. Twenty-two New Zealand rabbits suffering from endophthalmitis induced by Staphylococcus aureus (n = 10), non-infectious uveitis induced by lipopolysaccharide from Escherichia coli (LPS) (n = 10 animals) and control (n = 2) were included in the study. After eye inoculation, vitreous tissues were dissected and a fragment was submitted to dispersive Raman spectroscopy using near-infrared laser excitation (830 nm, 100 mW) and spectrograph/CCD camera for detection of Raman signal with integration time of 50 s. A routine was developed to classify the spectra of endophthalmitis and uveitis using principal components analysis (PCA) and Mahalanobis distance. The mean Raman spectra of tissues with uveitis and endophthalmitis showed several bands in the region of 800–1800 cm−1, which have been attributed to nucleic acids, amino acids and proteins from inflamed tissue and proliferating bacteria. The bands at 1004, 1258, 1339, 1451 and 1635 cm−1 showed statistically significant differences between both diseases. It was observed that principal components PC1, PC3 and PC4 showed statistically significant differences for the two tissue types, indicating that these PCs can be used to discriminate between the two groups. The diagnostic model showed 94% sensitivity, 95% specificity and 95% accuracy using PC3 × PC4. The Raman spectroscopy technique has been shown to be useful in differentiating uveitis and endophthalmitis in vitreous tissues in vitro, and these results may be clinically relevant for differentiating vitreous tissues to optimise the diagnosis of inflammatory and infectious vitreoretinal diseases.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-25T13:35:08Z No. of bitstreams: 1 Eglas Emanuel Rossi.pdf: 805100 bytes, checksum: 64ec4440b6fb504027975d6774684f02 (MD5)Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2014-10-10T14:22:11Z (GMT) No. of bitstreams: 1 Eglas Emanuel Rossi.pdf: 805100 bytes, checksum: 64ec4440b6fb504027975d6774684f02 (MD5)Made available in DSpace on 2014-10-10T14:22:12Z (GMT). No. of bitstreams: 1 Eglas Emanuel Rossi.pdf: 805100 bytes, checksum: 64ec4440b6fb504027975d6774684f02 (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1016/j.jphotobiol.2011.12.001reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBARaman spectroscopyVitreous bodyEndophthalmitis and uveitisDifferential diagnosisDiscriminant analysisPrincipal components analysisDifferential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysisJournal of Photochemistry and Photobiology B: Biologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALEglas Emanuel Rossi.pdfEglas Emanuel Rossi.pdfapplication/pdf805100https://repositorio.ufba.br/bitstream/ri/16368/1/Eglas%20Emanuel%20Rossi.pdf64ec4440b6fb504027975d6774684f02MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16368/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTEglas Emanuel Rossi.pdf.txtEglas Emanuel Rossi.pdf.txtExtracted texttext/plain38779https://repositorio.ufba.br/bitstream/ri/16368/3/Eglas%20Emanuel%20Rossi.pdf.txt9eb439eee67eaf7ef14e814cf8420bc2MD53ri/163682022-07-05 14:04:05.092oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:04:05Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
dc.title.alternative.pt_BR.fl_str_mv Journal of Photochemistry and Photobiology B: Biology
title Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
spellingShingle Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
Rossi, Eglas Emanuel
Raman spectroscopy
Vitreous body
Endophthalmitis and uveitis
Differential diagnosis
Discriminant analysis
Principal components analysis
title_short Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
title_full Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
title_fullStr Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
title_full_unstemmed Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
title_sort Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
author Rossi, Eglas Emanuel
author_facet Rossi, Eglas Emanuel
Pinheiro, Antonio Luiz Barbosa
Baltatu, Ovidiu C.
Pacheco, Marcos Tadeu Tavares
Silveira Jr., Landulfo
author_role author
author2 Pinheiro, Antonio Luiz Barbosa
Baltatu, Ovidiu C.
Pacheco, Marcos Tadeu Tavares
Silveira Jr., Landulfo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rossi, Eglas Emanuel
Pinheiro, Antonio Luiz Barbosa
Baltatu, Ovidiu C.
Pacheco, Marcos Tadeu Tavares
Silveira Jr., Landulfo
Rossi, Eglas Emanuel
Pinheiro, Antonio Luiz Barbosa
Baltatu, Ovidiu C.
Pacheco, Marcos Tadeu Tavares
Silveira Jr., Landulfo
dc.subject.por.fl_str_mv Raman spectroscopy
Vitreous body
Endophthalmitis and uveitis
Differential diagnosis
Discriminant analysis
Principal components analysis
topic Raman spectroscopy
Vitreous body
Endophthalmitis and uveitis
Differential diagnosis
Discriminant analysis
Principal components analysis
description Texto completo: acesso restrito. p. 73–78
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-10-10T14:22:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/16368
dc.identifier.issn.none.fl_str_mv 1011-1344
dc.identifier.number.pt_BR.fl_str_mv v. 107, n. 6
identifier_str_mv 1011-1344
v. 107, n. 6
url http://repositorio.ufba.br/ri/handle/ri/16368
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1016/j.jphotobiol.2011.12.001
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
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