Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/16368 |
Resumo: | Texto completo: acesso restrito. p. 73–78 |
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Rossi, Eglas EmanuelPinheiro, Antonio Luiz BarbosaBaltatu, Ovidiu C.Pacheco, Marcos Tadeu TavaresSilveira Jr., LandulfoRossi, Eglas EmanuelPinheiro, Antonio Luiz BarbosaBaltatu, Ovidiu C.Pacheco, Marcos Tadeu TavaresSilveira Jr., Landulfo2014-10-10T14:22:12Z20121011-1344http://repositorio.ufba.br/ri/handle/ri/16368v. 107, n. 6Texto completo: acesso restrito. p. 73–78Raman spectroscopy has been used for the diagnosis of various eye diseases. A diagnostic tool based on Raman spectroscopy has been developed to discriminate endophthalmitis from uveitis in vitreous tissues of rabbits’ eyes in vitro. Twenty-two New Zealand rabbits suffering from endophthalmitis induced by Staphylococcus aureus (n = 10), non-infectious uveitis induced by lipopolysaccharide from Escherichia coli (LPS) (n = 10 animals) and control (n = 2) were included in the study. After eye inoculation, vitreous tissues were dissected and a fragment was submitted to dispersive Raman spectroscopy using near-infrared laser excitation (830 nm, 100 mW) and spectrograph/CCD camera for detection of Raman signal with integration time of 50 s. A routine was developed to classify the spectra of endophthalmitis and uveitis using principal components analysis (PCA) and Mahalanobis distance. The mean Raman spectra of tissues with uveitis and endophthalmitis showed several bands in the region of 800–1800 cm−1, which have been attributed to nucleic acids, amino acids and proteins from inflamed tissue and proliferating bacteria. The bands at 1004, 1258, 1339, 1451 and 1635 cm−1 showed statistically significant differences between both diseases. It was observed that principal components PC1, PC3 and PC4 showed statistically significant differences for the two tissue types, indicating that these PCs can be used to discriminate between the two groups. The diagnostic model showed 94% sensitivity, 95% specificity and 95% accuracy using PC3 × PC4. The Raman spectroscopy technique has been shown to be useful in differentiating uveitis and endophthalmitis in vitreous tissues in vitro, and these results may be clinically relevant for differentiating vitreous tissues to optimise the diagnosis of inflammatory and infectious vitreoretinal diseases.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-25T13:35:08Z No. of bitstreams: 1 Eglas Emanuel Rossi.pdf: 805100 bytes, checksum: 64ec4440b6fb504027975d6774684f02 (MD5)Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2014-10-10T14:22:11Z (GMT) No. of bitstreams: 1 Eglas Emanuel Rossi.pdf: 805100 bytes, checksum: 64ec4440b6fb504027975d6774684f02 (MD5)Made available in DSpace on 2014-10-10T14:22:12Z (GMT). No. of bitstreams: 1 Eglas Emanuel Rossi.pdf: 805100 bytes, checksum: 64ec4440b6fb504027975d6774684f02 (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1016/j.jphotobiol.2011.12.001reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBARaman spectroscopyVitreous bodyEndophthalmitis and uveitisDifferential diagnosisDiscriminant analysisPrincipal components analysisDifferential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysisJournal of Photochemistry and Photobiology B: Biologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALEglas Emanuel Rossi.pdfEglas Emanuel Rossi.pdfapplication/pdf805100https://repositorio.ufba.br/bitstream/ri/16368/1/Eglas%20Emanuel%20Rossi.pdf64ec4440b6fb504027975d6774684f02MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16368/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTEglas Emanuel Rossi.pdf.txtEglas Emanuel Rossi.pdf.txtExtracted texttext/plain38779https://repositorio.ufba.br/bitstream/ri/16368/3/Eglas%20Emanuel%20Rossi.pdf.txt9eb439eee67eaf7ef14e814cf8420bc2MD53ri/163682022-07-05 14:04:05.092oai:repositorio.ufba.br:ri/16368VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBLgoKIFBlbG8gcHJvY2Vzc28gZGUgc3VibWlzc++/vW8gZGUgZG9jdW1lbnRvcywgbyBhdXRvciBvdSBzZXUgcmVwcmVzZW50YW50ZSBsZWdhbCwgYW8gYWNlaXRhciAKZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEsIGNvbmNlZGUgYW8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRhIEJhaGlhIApvIGRpcmVpdG8gZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCBkZSBwcmVzZXJ2Ye+/ve+/vW8uIApFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIGF1dG9yL2NvcHlyaWdodCwgbWFzIGVudGVuZGUgbyBkb2N1bWVudG8gCmNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4KCiBQYXJhIG9zIGRvY3VtZW50b3MgcHVibGljYWRvcyBjb20gcmVwYXNzZSBkZSBkaXJlaXRvcyBkZSBkaXN0cmlidWnvv73vv71vLCBlc3NlIHRlcm1vIGRlIGxpY2Vu77+9YSAKZW50ZW5kZSBxdWU6CgogTWFudGVuZG8gb3MgZGlyZWl0b3MgYXV0b3JhaXMsIHJlcGFzc2Fkb3MgYSB0ZXJjZWlyb3MsIGVtIGNhc28gZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8KcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIGludGVncmFsLCBtYXMgbGliZXJhIGFzIGluZm9ybWHvv73vv71lcyBzb2JyZSBvIGRvY3VtZW50bwooTWV0YWRhZG9zIGVzY3JpdGl2b3MpLgoKIERlc3RhIGZvcm1hLCBhdGVuZGVuZG8gYW9zIGFuc2Vpb3MgZGVzc2EgdW5pdmVyc2lkYWRlIGVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gCmFzIHJlc3Ryae+/ve+/vWVzIGltcG9zdGFzIHBlbG9zIGVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4KCiBQYXJhIGFzIHB1YmxpY2Hvv73vv71lcyBzZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgQWNlc3NvIEFiZXJ0bywgb3MgZGVw77+9c2l0b3MgCmNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gb3MgZGlyZWl0b3MgYXV0b3JhaXMsIG1hcyBtYW5077+9bSBhY2Vzc28gaXJyZXN0cml0byAKYW8gbWV0YWRhZG9zIGUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIGNvbnNlbnRpbWVudG8KIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgZXN0YXJlbSBlbSBpbmljaWF0aXZhcyBkZSBhY2Vzc28gYWJlcnRvLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:04:05Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis |
dc.title.alternative.pt_BR.fl_str_mv |
Journal of Photochemistry and Photobiology B: Biology |
title |
Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis |
spellingShingle |
Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis Rossi, Eglas Emanuel Raman spectroscopy Vitreous body Endophthalmitis and uveitis Differential diagnosis Discriminant analysis Principal components analysis |
title_short |
Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis |
title_full |
Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis |
title_fullStr |
Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis |
title_full_unstemmed |
Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis |
title_sort |
Differential diagnosis between experimental endophthalmitis and uveitis in vitreous with Raman spectroscopy and principal components analysis |
author |
Rossi, Eglas Emanuel |
author_facet |
Rossi, Eglas Emanuel Pinheiro, Antonio Luiz Barbosa Baltatu, Ovidiu C. Pacheco, Marcos Tadeu Tavares Silveira Jr., Landulfo |
author_role |
author |
author2 |
Pinheiro, Antonio Luiz Barbosa Baltatu, Ovidiu C. Pacheco, Marcos Tadeu Tavares Silveira Jr., Landulfo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rossi, Eglas Emanuel Pinheiro, Antonio Luiz Barbosa Baltatu, Ovidiu C. Pacheco, Marcos Tadeu Tavares Silveira Jr., Landulfo Rossi, Eglas Emanuel Pinheiro, Antonio Luiz Barbosa Baltatu, Ovidiu C. Pacheco, Marcos Tadeu Tavares Silveira Jr., Landulfo |
dc.subject.por.fl_str_mv |
Raman spectroscopy Vitreous body Endophthalmitis and uveitis Differential diagnosis Discriminant analysis Principal components analysis |
topic |
Raman spectroscopy Vitreous body Endophthalmitis and uveitis Differential diagnosis Discriminant analysis Principal components analysis |
description |
Texto completo: acesso restrito. p. 73–78 |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2014-10-10T14:22:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/16368 |
dc.identifier.issn.none.fl_str_mv |
1011-1344 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 107, n. 6 |
identifier_str_mv |
1011-1344 v. 107, n. 6 |
url |
http://repositorio.ufba.br/ri/handle/ri/16368 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/ 10.1016/j.jphotobiol.2011.12.001 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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Universidade Federal da Bahia (UFBA) |
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UFBA |
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Repositório Institucional da UFBA |
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Repositório Institucional da UFBA |
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