RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil

Detalhes bibliográficos
Autor(a) principal: Guimarães, Alaíse Gil
Data de Publicação: 2011
Outros Autores: Santos, C. A., Almeida, F. S., Abrahão, Wanda Moscalewski, Arantes, O. M. N., Vilas-Bôas, Gislayne Trindade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5452
Resumo: texto completo: acesso restrito. p.277–283.
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spelling Guimarães, Alaíse GilSantos, C. A.Almeida, F. S.Abrahão, Wanda MoscalewskiArantes, O. M. N.Vilas-Bôas, Gislayne TrindadeGuimarães, Alaíse GilSantos, C. A.Almeida, F. S.Abrahão, Wanda MoscalewskiArantes, O. M. N.Vilas-Bôas, Gislayne Trindade2012-02-25T16:23:47Z20111879-346http://www.repositorio.ufba.br/ri/handle/ri/5452v. 151.texto completo: acesso restrito. p.277–283.Twenty-three Bacillus cereus isolates from food poisoning outbreaks associated with a diarrheal-type syndrome, fourteen foodborne isolates not associated with food poisoning and fifteen isolates from Brazilian soil samples were analyzed for the presence and genetic diversity (by RE-PCR) of the virulence genes ces (emetic toxin, cereulide), plcR–papR (pleiotropic regulator PlcR and peptide PapR), nheA (a component of the NHE complex), bceT (diarrheal enterotoxin bc-D-ENT), gyrB (B subunit of DNA gyrase), cytK-2 (necrotic enterotoxin cytotoxin K-2), and plcA (phosphatidylcholine-specific phospholipase C). Additionally, these isolates were phenotypically characterized for motility, hemolytic and lecithinase activities, as well as HBL enterotoxin production. The group of isolates associated with food poisoning had the highest occurrence of the phenotypically analyzed factors and the most frequent occurrence and highest genetic diversity of the plcR–papR, nheA, bceT, cytK-2, plcA, and gyrB genes. An analysis of molecular variance (AMOVA), in which all loci were analyzed, demonstrated that the genetic variation intragroup of isolates (92%) was significantly higher than that intergroup (8%) (Pb0.05). These results were corroborated by an analysis of the genetic differentiation between the groups, which was low/moderate, the result of a high degree of allele sharing. Our results suggest that B. cereus isolates with the potential to cause food poisoning outbreaks do not have a specific genetic profile characterized by the presence of a particular gene or allele among the genes assessed. On the contrary, different combinations of genes encoding virulence factors may be present in different isolates of B. cereus that potentially cause food poisoning outbreaks.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-25T16:23:47Z No. of bitstreams: 1 RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil.pdf: 138349 bytes, checksum: bf4982f64a0f14e3e6a29fd627402905 (MD5)Made available in DSpace on 2012-02-25T16:23:47Z (GMT). No. of bitstreams: 1 RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil.pdf: 138349 bytes, checksum: bf4982f64a0f14e3e6a29fd627402905 (MD5) Previous issue date: 2011doi:10.1016/j.ijfoodmicro.2011.09.008reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABacillus cereusFood poisoning outbreaksFoodborneSoilVirulence factorsRE-PCRRE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from BrazilInternational Journal of Food Microbiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINALRE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil.pdfRE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil.pdfapplication/pdf138349https://repositorio.ufba.br/bitstream/ri/5452/1/RE-PCR%20variability%20and%20toxigenic%20profile%20of%20food%20poisoning%2c%20foodborne%20and%20soil-associated%20Bacillus%20cereus%20isolates%20from%20Brazil.pdfbf4982f64a0f14e3e6a29fd627402905MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5452/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTRE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil.pdf.txtRE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil.pdf.txtExtracted texttext/plain43205https://repositorio.ufba.br/bitstream/ri/5452/3/RE-PCR%20variability%20and%20toxigenic%20profile%20of%20food%20poisoning%2c%20foodborne%20and%20soil-associated%20Bacillus%20cereus%20isolates%20from%20Brazil.pdf.txt2ebd348434ad3345c255d026b561bcd1MD53ri/54522022-07-05 14:03:13.075oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:13Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil
dc.title.alternative.pt_BR.fl_str_mv International Journal of Food Microbiology
title RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil
spellingShingle RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil
Guimarães, Alaíse Gil
Bacillus cereus
Food poisoning outbreaks
Foodborne
Soil
Virulence factors
RE-PCR
title_short RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil
title_full RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil
title_fullStr RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil
title_full_unstemmed RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil
title_sort RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil
author Guimarães, Alaíse Gil
author_facet Guimarães, Alaíse Gil
Santos, C. A.
Almeida, F. S.
Abrahão, Wanda Moscalewski
Arantes, O. M. N.
Vilas-Bôas, Gislayne Trindade
author_role author
author2 Santos, C. A.
Almeida, F. S.
Abrahão, Wanda Moscalewski
Arantes, O. M. N.
Vilas-Bôas, Gislayne Trindade
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, Alaíse Gil
Santos, C. A.
Almeida, F. S.
Abrahão, Wanda Moscalewski
Arantes, O. M. N.
Vilas-Bôas, Gislayne Trindade
Guimarães, Alaíse Gil
Santos, C. A.
Almeida, F. S.
Abrahão, Wanda Moscalewski
Arantes, O. M. N.
Vilas-Bôas, Gislayne Trindade
dc.subject.por.fl_str_mv Bacillus cereus
Food poisoning outbreaks
Foodborne
Soil
Virulence factors
RE-PCR
topic Bacillus cereus
Food poisoning outbreaks
Foodborne
Soil
Virulence factors
RE-PCR
description texto completo: acesso restrito. p.277–283.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-02-25T16:23:47Z
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dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5452
dc.identifier.issn.none.fl_str_mv 1879-346
dc.identifier.number.pt_BR.fl_str_mv v. 151.
identifier_str_mv 1879-346
v. 151.
url http://www.repositorio.ufba.br/ri/handle/ri/5452
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dc.source.pt_BR.fl_str_mv doi:10.1016/j.ijfoodmicro.2011.09.008
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