Invasion of Ureaplasma diversum in Hep-2 cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/5857 |
Resumo: | p.1-8 |
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Marques, Lucas MirandaUeno, Priscilla MegumiBuzinhani, MelissaCortez, Beatriz de AraujoL. Neto, RenataYamaguti, MaurícioOliveira, Rosângela C.Guimarães, Ana Márcia S.Monezi, Telma AlvesBraga Júnior, Antônio Carlos RicardoMarques, Lucas MirandaUeno, Priscilla MegumiBuzinhani, MelissaCortez, Beatriz de AraujoL. Neto, RenataYamaguti, MaurícioOliveira, Rosângela C.Guimarães, Ana Márcia S.Monezi, Telma AlvesBraga Júnior, Antônio Carlos Ricardo2012-05-14T19:29:39Z2012-05-14T19:29:39Z20101471-2180http://www.repositorio.ufba.br/ri/handle/ri/5857v.10, n.83p.1-8Background: Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay. Results: The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma. Conclusions: The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-05-14T19:29:39Z No. of bitstreams: 1 (18)1471-2180-10-83.pdf: 3563641 bytes, checksum: 9f06f596a9d50ff89ab888342ec9c95f (MD5)Made available in DSpace on 2012-05-14T19:29:39Z (GMT). No. of bitstreams: 1 (18)1471-2180-10-83.pdf: 3563641 bytes, checksum: 9f06f596a9d50ff89ab888342ec9c95f (MD5) Previous issue date: 2010BioMed10.1186/1471-2180-10-83reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAInvasion of Ureaplasma diversum in Hep-2 cellsBMC Microbiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL(18)1471-2180-10-83.pdf(18)1471-2180-10-83.pdfapplication/pdf3563641https://repositorio.ufba.br/bitstream/ri/5857/1/%2818%291471-2180-10-83.pdf9f06f596a9d50ff89ab888342ec9c95fMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5857/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT(18)1471-2180-10-83.pdf.txt(18)1471-2180-10-83.pdf.txtExtracted texttext/plain31366https://repositorio.ufba.br/bitstream/ri/5857/3/%2818%291471-2180-10-83.pdf.txtdaeaf0243527478c28b44793dfb13390MD53ri/58572022-07-05 14:03:13.548oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:13Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Invasion of Ureaplasma diversum in Hep-2 cells |
| dc.title.alternative.pt_BR.fl_str_mv |
BMC Microbiology |
| title |
Invasion of Ureaplasma diversum in Hep-2 cells |
| spellingShingle |
Invasion of Ureaplasma diversum in Hep-2 cells Marques, Lucas Miranda |
| title_short |
Invasion of Ureaplasma diversum in Hep-2 cells |
| title_full |
Invasion of Ureaplasma diversum in Hep-2 cells |
| title_fullStr |
Invasion of Ureaplasma diversum in Hep-2 cells |
| title_full_unstemmed |
Invasion of Ureaplasma diversum in Hep-2 cells |
| title_sort |
Invasion of Ureaplasma diversum in Hep-2 cells |
| author |
Marques, Lucas Miranda |
| author_facet |
Marques, Lucas Miranda Ueno, Priscilla Megumi Buzinhani, Melissa Cortez, Beatriz de Araujo L. Neto, Renata Yamaguti, Maurício Oliveira, Rosângela C. Guimarães, Ana Márcia S. Monezi, Telma Alves Braga Júnior, Antônio Carlos Ricardo |
| author_role |
author |
| author2 |
Ueno, Priscilla Megumi Buzinhani, Melissa Cortez, Beatriz de Araujo L. Neto, Renata Yamaguti, Maurício Oliveira, Rosângela C. Guimarães, Ana Márcia S. Monezi, Telma Alves Braga Júnior, Antônio Carlos Ricardo |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Marques, Lucas Miranda Ueno, Priscilla Megumi Buzinhani, Melissa Cortez, Beatriz de Araujo L. Neto, Renata Yamaguti, Maurício Oliveira, Rosângela C. Guimarães, Ana Márcia S. Monezi, Telma Alves Braga Júnior, Antônio Carlos Ricardo Marques, Lucas Miranda Ueno, Priscilla Megumi Buzinhani, Melissa Cortez, Beatriz de Araujo L. Neto, Renata Yamaguti, Maurício Oliveira, Rosângela C. Guimarães, Ana Márcia S. Monezi, Telma Alves Braga Júnior, Antônio Carlos Ricardo |
| description |
p.1-8 |
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2010 |
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2010 |
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2012-05-14T19:29:39Z |
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2012-05-14T19:29:39Z |
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info:eu-repo/semantics/publishedVersion |
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1471-2180 |
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v.10, n.83 |
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1471-2180 v.10, n.83 |
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BioMed |
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BioMed |
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10.1186/1471-2180-10-83 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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