Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos

Detalhes bibliográficos
Autor(a) principal: Nascimento, Iracema Andrade
Data de Publicação: 2005
Outros Autores: Leite, Maria Bernadete Neiva Lemos, Araujo, Milena Maria Sampaio de, Sansone, Giovanni, Pereira, Solange Andrade, Espírito Santo, Maristela do
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13645
Resumo: Texto completo: acesso restrito. p. 113–117
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spelling Nascimento, Iracema AndradeLeite, Maria Bernadete Neiva LemosAraujo, Milena Maria Sampaio deSansone, GiovanniPereira, Solange AndradeEspírito Santo, Maristela doNascimento, Iracema AndradeLeite, Maria Bernadete Neiva LemosAraujo, Milena Maria Sampaio deSansone, GiovanniPereira, Solange AndradeEspírito Santo, Maristela do2013-11-14T20:04:08Z20050011-2240http://repositorio.ufba.br/ri/handle/ri/13645v. 51, n. 1Texto completo: acesso restrito. p. 113–117Cryopreservation is a valuable tool for aquaculture by providing continuous seed production, regardless of the spawning seasons. This study aimed to select the least toxic among the cryoprotectants dimethyl sulfoxide (Me2SO), propylene glycol (PG), and methanol (MET) based on their toxicological effects on Crassostrea rhizophorae gametes and trochophores. They were exposed for 10, 20, and 30 min to a range of concentrations of those cryoprotectants. The endpoint was EC15–24 h (effective concentration which causes abnormalities in 15% of the population exposed to the cryoprotectants for 24 h), recently determined as the chronic value (the concentration at which chronic effects are first observed) for C. rhizophorae embryonic phases. There were no significant differences (p > 0.05) among the exposure times in Me2SO toxic effects to either gametes or trochophores. For MET, the increase in exposure time resulted in higher toxicity for gametes, but not for trochophores, while for PG there was a significant (p > 0.05) increase in toxicity with the increase of exposure for trochophores and spermatozoa, but not for oocytes. For gametes, MET was the most toxic among the cryoprotectants, while PG was the most toxic for trochophores.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-11-13T11:25:55Z No. of bitstreams: 1 Iracema A. Nascimento.pdf: 275118 bytes, checksum: ffc717827e33b183fd7e905f7b20121d (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-14T20:04:07Z (GMT) No. of bitstreams: 1 Iracema A. Nascimento.pdf: 275118 bytes, checksum: ffc717827e33b183fd7e905f7b20121d (MD5)Made available in DSpace on 2013-11-14T20:04:08Z (GMT). No. of bitstreams: 1 Iracema A. Nascimento.pdf: 275118 bytes, checksum: ffc717827e33b183fd7e905f7b20121d (MD5) Previous issue date: 2005http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.cryobiol.2005.04.006reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACryoprotectantsToxic effectsCryopreservation protocolsOyster gametesTrochophoresSelection of cryoprotectants based on their toxic effects on oyster gametes and embryosCryobiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALIracema A. Nascimento.pdfIracema A. 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dc.title.pt_BR.fl_str_mv Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos
dc.title.alternative.pt_BR.fl_str_mv Cryobiology
title Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos
spellingShingle Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos
Nascimento, Iracema Andrade
Cryoprotectants
Toxic effects
Cryopreservation protocols
Oyster gametes
Trochophores
title_short Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos
title_full Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos
title_fullStr Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos
title_full_unstemmed Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos
title_sort Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos
author Nascimento, Iracema Andrade
author_facet Nascimento, Iracema Andrade
Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Sansone, Giovanni
Pereira, Solange Andrade
Espírito Santo, Maristela do
author_role author
author2 Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Sansone, Giovanni
Pereira, Solange Andrade
Espírito Santo, Maristela do
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Nascimento, Iracema Andrade
Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Sansone, Giovanni
Pereira, Solange Andrade
Espírito Santo, Maristela do
Nascimento, Iracema Andrade
Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Sansone, Giovanni
Pereira, Solange Andrade
Espírito Santo, Maristela do
dc.subject.por.fl_str_mv Cryoprotectants
Toxic effects
Cryopreservation protocols
Oyster gametes
Trochophores
topic Cryoprotectants
Toxic effects
Cryopreservation protocols
Oyster gametes
Trochophores
description Texto completo: acesso restrito. p. 113–117
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2013-11-14T20:04:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13645
dc.identifier.issn.none.fl_str_mv 0011-2240
dc.identifier.number.pt_BR.fl_str_mv v. 51, n. 1
identifier_str_mv 0011-2240
v. 51, n. 1
url http://repositorio.ufba.br/ri/handle/ri/13645
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.cryobiol.2005.04.006
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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reponame_str Repositório Institucional da UFBA
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