Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols

Detalhes bibliográficos
Autor(a) principal: Sansone, Giovanni
Data de Publicação: 2005
Outros Autores: Nascimento, Iracema Andrade, Leite, Maria Bernadete Neiva Lemos, Araujo, Milena Maria Sampaio de, Pereira, Solange Andrade, Mariani, Ana Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/17093
Resumo: p. 11-18
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spelling Sansone, GiovanniNascimento, Iracema AndradeLeite, Maria Bernadete Neiva LemosAraujo, Milena Maria Sampaio dePereira, Solange AndradeMariani, Ana Maria2015-03-06T18:14:56Z2015-03-06T18:14:56Z20051679-2343http://repositorio.ufba.br/ri/handle/ri/17093v. 13, n. 1p. 11-18Aquaculture development is dependent on continuous seed production, regardless of the spawning season. Cryopreservation can therefore, be a valuable tool for achieving seed availability. The benefits of cryopreservation have been demonstrated for mammals, fish and some achievements have been reported for invertebrates; however, cryopreservation has not yet contributed significantly to oyster rearing. The literature on this topic demonstrates that an optimal cryopreservation method developed for one species is not always applicable to another. One of the reasons is that there are differences among species in the toxicological responses of gametes and embryos to cryoprotectants. The aim of this study was to determine the toxic effects of cryoprotectants dimethylsulfoxide (Me2SO), glycerol (G), ethylene glycerol (EG), propylene glycerol (PG) and methanol (MET) on Crassostrea rhizophorae gametes and embryos). Gametes (oocytes and spermatozoa) and embryos (trochophores) were exposed to a range of concentrations of each cryoprotectant for 10, 20 and 30 minutes. Based on the EC50-24h values there were no significant differences (p > 0.05) among the exposure times in toxic effects to either gametes or embryos. The trochophores were relatively resistant to cryoprotectant exposure, while oyster gametes became increasingly susceptible to the cryoprotectants as concentration levels were increased. Critical values (EC50-24h) of cryoprotectants were markedly different for gametes and embryos. For gametes, both G and MET were more toxic (EC50-24h respectively of 3.46 and 4.52% for oocytes, and 2.07 and 11.21% for spermatozoa) than EG, PG and Me2SO. However, PG (EC50-24h = 23.56%) and EG (EC50-24h = 45.18%) were more toxic for trochophores than Me2SO (EC50-24h = 54.25) or MET(EC50-24h = 55.63%). These results show the importance of previous toxicological studies for cryoprotectant selection as a preliminary step towards establishing cryopreservation protocols.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2015-03-04T15:25:41Z No. of bitstreams: 1 23-Biociências (Fisiologia)13-01p 11-18, 2005.pdf: 4707239 bytes, checksum: a1af8cf14ac976b4b153454e93621d1b (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2015-03-06T18:14:56Z (GMT) No. of bitstreams: 1 23-Biociências (Fisiologia)13-01p 11-18, 2005.pdf: 4707239 bytes, checksum: a1af8cf14ac976b4b153454e93621d1b (MD5)Made available in DSpace on 2015-03-06T18:14:56Z (GMT). No. of bitstreams: 1 23-Biociências (Fisiologia)13-01p 11-18, 2005.pdf: 4707239 bytes, checksum: a1af8cf14ac976b4b153454e93621d1b (MD5) Previous issue date: 2005CrioprotetoresEfeitos tóxicosProtocolos da criopreservaçãoGametas de ostraTrocóforasToxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocolsRevista Brasileira de Biociênciasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAORIGINAL23-Biociências (Fisiologia)13-01p 11-18, 2005.pdf23-Biociências (Fisiologia)13-01p 11-18, 2005.pdfapplication/pdf4707239https://repositorio.ufba.br/bitstream/ri/17093/1/23-Bioci%c3%aancias%20%28Fisiologia%2913-01p%2011-18%2c%202005.pdfa1af8cf14ac976b4b153454e93621d1bMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/17093/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXT23-Biociências (Fisiologia)13-01p 11-18, 2005.pdf.txt23-Biociências (Fisiologia)13-01p 11-18, 2005.pdf.txtExtracted texttext/plain8https://repositorio.ufba.br/bitstream/ri/17093/3/23-Bioci%c3%aancias%20%28Fisiologia%2913-01p%2011-18%2c%202005.pdf.txt8d1b69dd9bdc9df4a8073c7a8193c7afMD53ri/170932022-03-10 14:02:34.062oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-03-10T17:02:34Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols
dc.title.alternative.pt_BR.fl_str_mv Revista Brasileira de Biociências
title Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols
spellingShingle Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols
Sansone, Giovanni
Crioprotetores
Efeitos tóxicos
Protocolos da criopreservação
Gametas de ostra
Trocóforas
title_short Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols
title_full Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols
title_fullStr Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols
title_full_unstemmed Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols
title_sort Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols
author Sansone, Giovanni
author_facet Sansone, Giovanni
Nascimento, Iracema Andrade
Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Pereira, Solange Andrade
Mariani, Ana Maria
author_role author
author2 Nascimento, Iracema Andrade
Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Pereira, Solange Andrade
Mariani, Ana Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Sansone, Giovanni
Nascimento, Iracema Andrade
Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Pereira, Solange Andrade
Mariani, Ana Maria
dc.subject.por.fl_str_mv Crioprotetores
Efeitos tóxicos
Protocolos da criopreservação
Gametas de ostra
Trocóforas
topic Crioprotetores
Efeitos tóxicos
Protocolos da criopreservação
Gametas de ostra
Trocóforas
description p. 11-18
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2015-03-06T18:14:56Z
dc.date.available.fl_str_mv 2015-03-06T18:14:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/17093
dc.identifier.issn.none.fl_str_mv 1679-2343
dc.identifier.number.pt_BR.fl_str_mv v. 13, n. 1
identifier_str_mv 1679-2343
v. 13, n. 1
url http://repositorio.ufba.br/ri/handle/ri/17093
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.country.fl_str_mv Brasil
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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