Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses

Detalhes bibliográficos
Autor(a) principal: Brodskyn, Claudia Ida
Data de Publicação: 2002
Outros Autores: Patricio, Julie, Oliveira, Rubem, Lobo, Lucas, Arnholdt, Andrea, Previato, Lucia Mendonça, Barral, Aldina Maria Prado, Barral-Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7468
Resumo: Texto completo: acesso restrito. p. 3736-3743
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spelling Brodskyn, Claudia IdaPatricio, JulieOliveira, RubemLobo, LucasArnholdt, AndreaPreviato, Lucia MendonçaBarral, Aldina Maria PradoBarral-Netto, ManoelBrodskyn, Claudia IdaPatricio, JulieOliveira, RubemLobo, LucasArnholdt, AndreaPreviato, Lucia MendonçaBarral, Aldina Maria PradoBarral-Netto, Manoel2012-12-12T17:58:20Z2002-070019-9567http://www.repositorio.ufba.br/ri/handle/ri/7468v. 70, n. 7Texto completo: acesso restrito. p. 3736-3743To investigate the possible effects of glycoinositolphospholipid (GIPL) from Trypanosoma cruzi on human antigen presenting cells, we tested their effects on lipopolysaccharide (LPS)-stimulated human macrophages and dendritic cells (DC). Human macrophages or DC were incubated with GIPL (50 μg/ml) and LPS (500 pg/ml) and tumor necrosis factor alpha (TNF-α), interleukin 8 (IL-8), IL-10, and IL-12p40 levels in supernatants were analyzed by enzyme-linked immunosorbent assay. TNF-α, IL-10, and IL-12 secretion were significantly decreased by GIPL both in macrophages and DC. In contrast, GIPL did not alter IL-8 production. We also analyzed the expression of CD80, CD86, HLA-DR, CD40, and CD57 on the macrophage surface after stimulation with LPS in the presence or absence of T. cruzi GIPL. GIPL led to a down-regulation in the expression of all tested molecules. We additionally examined the influence of T. cruzi GIPL on the response of human DC to LPS. LPS-induced HLA-DR, CD83, and CD86 up-regulation was significantly inhibited by GIPL. A slight down-regulation in CD80 and CD40 expression on DC surfaces in the presence of GIPL was also noticed. Similarly, GIPL led to down-modulation of CD83, CD80, CD86, and HLA-DR surface expression and TNF-α and IL-10 production when DC were stimulated by CD40L. The ceramide portion of GIPL was responsible for most of the activity exhibited by the whole molecule. Considering the important role of the immune response in determining the fate of the host-parasite relationship, the immunoregulatory activities of T. cruzi GIPL are potentially important for parasite evasion and then pathogenesis of infection with protozoan parasites.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2012-12-12T17:58:20Z No. of bitstreams: 1 3736.full.pdf: 641899 bytes, checksum: 8dad74ddd272deb332accce75b12176c (MD5)Made available in DSpace on 2012-12-12T17:58:20Z (GMT). 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dc.title.pt_BR.fl_str_mv Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
dc.title.alternative.pt_BR.fl_str_mv Infection and Immunity
title Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
spellingShingle Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
Brodskyn, Claudia Ida
title_short Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title_full Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title_fullStr Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title_full_unstemmed Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title_sort Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
author Brodskyn, Claudia Ida
author_facet Brodskyn, Claudia Ida
Patricio, Julie
Oliveira, Rubem
Lobo, Lucas
Arnholdt, Andrea
Previato, Lucia Mendonça
Barral, Aldina Maria Prado
Barral-Netto, Manoel
author_role author
author2 Patricio, Julie
Oliveira, Rubem
Lobo, Lucas
Arnholdt, Andrea
Previato, Lucia Mendonça
Barral, Aldina Maria Prado
Barral-Netto, Manoel
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brodskyn, Claudia Ida
Patricio, Julie
Oliveira, Rubem
Lobo, Lucas
Arnholdt, Andrea
Previato, Lucia Mendonça
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Patricio, Julie
Oliveira, Rubem
Lobo, Lucas
Arnholdt, Andrea
Previato, Lucia Mendonça
Barral, Aldina Maria Prado
Barral-Netto, Manoel
description Texto completo: acesso restrito. p. 3736-3743
publishDate 2002
dc.date.issued.fl_str_mv 2002-07
dc.date.accessioned.fl_str_mv 2012-12-12T17:58:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7468
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.number.pt_BR.fl_str_mv v. 70, n. 7
identifier_str_mv 0019-9567
v. 70, n. 7
url http://www.repositorio.ufba.br/ri/handle/ri/7468
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1128/IAI.70.7.3736-3743.2002
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
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