Analysis of schistosoma mansoni population structure using total fecal egg sampling

Detalhes bibliográficos
Autor(a) principal: Blank, Walter A.
Data de Publicação: 2009
Outros Autores: Reis, Eliana A. G., Thiong'o, Fred W., Braghiroli, João Freitas Melro, Santos, Jarbas M., Melo, Paulo Roberto Santana de, Guimarães, Isabel C. S., Silva, Luciano K., Carmo, Theomira Mauadie Azevedo, Reis, Mitermayer Galvão dos, Blanton, Ronald Edward
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13354
Resumo: p. 881-889
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spelling Blank, Walter A.Reis, Eliana A. G.Thiong'o, Fred W.Braghiroli, João Freitas MelroSantos, Jarbas M.Melo, Paulo Roberto Santana deGuimarães, Isabel C. S.Silva, Luciano K.Carmo, Theomira Mauadie AzevedoReis, Mitermayer Galvão dosBlanton, Ronald EdwardBlank, Walter A.Reis, Eliana A. G.Thiong'o, Fred W.Braghiroli, João Freitas MelroSantos, Jarbas M.Melo, Paulo Roberto Santana deGuimarães, Isabel C. S.Silva, Luciano K.Carmo, Theomira Mauadie AzevedoReis, Mitermayer Galvão dosBlanton, Ronald Edward2013-10-30T18:02:25Z2013-10-30T18:02:25Z20090022-3395http://repositorio.ufba.br/ri/handle/ri/13354v. 95, n. 4p. 881-889Many parasite populations are difficult to sample because they are not uniformly distributed between several host species and are often not easily collected from the living host, thereby limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of eggs, in aggregate, from the stools of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults. Here, we show that genotyping of parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. Microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Preferential amplification of smaller alleles, and stutter PCR products, had negligible effect on measurement of genetic differentiation. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per infected individual and by reducing bias.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-10-02T13:01:26Z No. of bitstreams: 1 10.1645-GE-1895.1.pdf: 2097152 bytes, checksum: b46e88480fc85a2466e6e980a06407cd (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-10-30T18:02:25Z (GMT) No. of bitstreams: 1 10.1645-GE-1895.1.pdf: 2097152 bytes, checksum: b46e88480fc85a2466e6e980a06407cd (MD5)Made available in DSpace on 2013-10-30T18:02:25Z (GMT). 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dc.title.pt_BR.fl_str_mv Analysis of schistosoma mansoni population structure using total fecal egg sampling
dc.title.alternative.pt_BR.fl_str_mv Journal of Parasitology
title Analysis of schistosoma mansoni population structure using total fecal egg sampling
spellingShingle Analysis of schistosoma mansoni population structure using total fecal egg sampling
Blank, Walter A.
title_short Analysis of schistosoma mansoni population structure using total fecal egg sampling
title_full Analysis of schistosoma mansoni population structure using total fecal egg sampling
title_fullStr Analysis of schistosoma mansoni population structure using total fecal egg sampling
title_full_unstemmed Analysis of schistosoma mansoni population structure using total fecal egg sampling
title_sort Analysis of schistosoma mansoni population structure using total fecal egg sampling
author Blank, Walter A.
author_facet Blank, Walter A.
Reis, Eliana A. G.
Thiong'o, Fred W.
Braghiroli, João Freitas Melro
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano K.
Carmo, Theomira Mauadie Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
author_role author
author2 Reis, Eliana A. G.
Thiong'o, Fred W.
Braghiroli, João Freitas Melro
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano K.
Carmo, Theomira Mauadie Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Blank, Walter A.
Reis, Eliana A. G.
Thiong'o, Fred W.
Braghiroli, João Freitas Melro
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano K.
Carmo, Theomira Mauadie Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
Blank, Walter A.
Reis, Eliana A. G.
Thiong'o, Fred W.
Braghiroli, João Freitas Melro
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano K.
Carmo, Theomira Mauadie Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
description p. 881-889
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2013-10-30T18:02:25Z
dc.date.available.fl_str_mv 2013-10-30T18:02:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13354
dc.identifier.issn.none.fl_str_mv 0022-3395
dc.identifier.number.pt_BR.fl_str_mv v. 95, n. 4
identifier_str_mv 0022-3395
v. 95, n. 4
url http://repositorio.ufba.br/ri/handle/ri/13354
dc.language.iso.fl_str_mv eng
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